Chromosome Dynamics in the Yeast Interphase Nucleus

Little is known about the dynamics of chromosomes in interphase nuclei. By tagging four chromosomal regions with a green fluorescent protein fusion to lac repressor, we monitored the movement and subnuclear position of specific sites in the yeast genome, sampling at short time intervals. We found that early and late origins of replication are highly mobile in G1 phase, frequently moving at or faster than 0.5 micrometers/10 seconds, in an energy-dependent fashion. The rapid diffusive movement of chromatin detected in G1 becomes constrained in S phase through a mechanism dependent on active DNA replication. In contrast, telomeres and centromeres provide replication-independent constraint on chromatin movement in both G1 and S phases.

[1]  John W. Sedat,et al.  Multiple regimes of constrained chromosome motion are regulated in the interphase Drosophila nucleus , 2001, Current Biology.

[2]  P. Silver,et al.  Localization of yeast telomeres to the nuclear periphery is separable from transcriptional repression and telomere stability functions. , 2001, Molecular cell.

[3]  T Misteli,et al.  Functional architecture in the cell nucleus. , 2001, The Biochemical journal.

[4]  J. Nichols,et al.  Energy-dependent Flip of Fluorescence-labeled Phospholipids Is Regulated by Nutrient Starvation and Transcription Factors, PDR1 and PDR3 * , 2001, The Journal of Biological Chemistry.

[5]  Andrew S. Belmont,et al.  Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator , 2001, Nature Cell Biology.

[6]  S. Gasser,et al.  The Positioning and Dynamics of Origins of Replication in the Budding Yeast Nucleus , 2001, The Journal of cell biology.

[7]  D. Spector,et al.  Visualization of gene activity in living cells , 2000, Nature Cell Biology.

[8]  W. Bickmore,et al.  Re-modelling of nuclear architecture in quiescent and senescent human fibroblasts , 2000, Current Biology.

[9]  Hiroshi Kimura,et al.  Direct Imaging of DNA in Living Cells Reveals the Dynamics of Chromosome Formation , 1999, The Journal of cell biology.

[10]  J. Diffley,et al.  A Mec1- and Rad53-dependent checkpoint controls late-firing origins of DNA replication , 1998, Nature.

[11]  S. Henikoff,et al.  Large-scale Chromosomal Movements During Interphase Progression in Drosophila , 1998, The Journal of cell biology.

[12]  Ernst H. K. Stelzer,et al.  Structure and dynamics of human interphase chromosome territories in vivo , 1998, Human Genetics.

[13]  L. Rubbi,et al.  Chromatin structure of the Saccharomyces cerevisiae DNA topoisomerase I promoter in different growth phases. , 1997, The Biochemical journal.

[14]  A. Murray,et al.  Interphase chromosomes undergo constrained diffusional motion in living cells , 1997, Current Biology.

[15]  P. Brown,et al.  Exploring the metabolic and genetic control of gene expression on a genomic scale. , 1997, Science.

[16]  T. Weinert,et al.  Characterization of the Checkpoint Gene RAD53/MEC2 in Saccharomyces cerevisiae , 1997, Yeast.

[17]  S. Gasser,et al.  ORC-dependent and origin-specific initiation of DNA replication at defined foci in isolated yeast nuclei. , 1997, Genes & development.

[18]  D. Arndt-Jovin,et al.  The Dynamic Nuclear Redistribution of an hnRNP K-homologous Protein during Drosophila Embryo Development and Heat Shock. Flexibility of Transcription Sites In Vivo , 1997, The Journal of cell biology.

[19]  V. Doye,et al.  Dynamics of Nuclear Pore Distribution in Nucleoporin Mutant Yeast Cells , 1997, The Journal of cell biology.

[20]  A S Belmont,et al.  In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition , 1996, The Journal of cell biology.

[21]  Andrew W. Murray,et al.  GFP tagging of budding yeast chromosomes reveals that protein–protein interactions can mediate sister chromatid cohesion , 1996, Current Biology.

[22]  K M Hahn,et al.  Dynamic elastic behavior of alpha-satellite DNA domains visualized in situ in living human cells , 1996, The Journal of cell biology.

[23]  H. Scherthan,et al.  The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae , 1996, The Journal of cell biology.

[24]  J. Kilmartin,et al.  Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication , 1996, The Journal of cell biology.

[25]  Bruce Stillman,et al.  ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome , 1995, Cell.

[26]  R A Laskey,et al.  Regulation of eukaryotic DNA replication. , 1994, Annual review of biochemistry.

[27]  S. Bell,et al.  Yeast origin recognition complex functions in transcription silencing and DNA replication. , 1993, Science.

[28]  J. Rine,et al.  Origin recognition complex (ORC) in transcriptional silencing and DNA replication in S. cerevisiae. , 1993, Science.

[29]  I. Herskowitz,et al.  Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system. , 1993, Science.

[30]  J. Hamlin,et al.  A general protocol for evaluating the specific effects of DNA replication inhibitors. , 1993, Nucleic acids research.

[31]  C Cremer,et al.  Role of chromosome territories in the functional compartmentalization of the cell nucleus. , 1993, Cold Spring Harbor symposia on quantitative biology.

[32]  G. Edelman,et al.  Evidence for participation of a multiprotein complex in yeast DNA replication in vitro. , 1984, The Journal of biological chemistry.