Inheritance of characters involved in fruit quality in a citrus interspecific allotetraploid somatic hybrid.

The main components of citrus fruit quality (organic acids, sugars, and aromatic compounds) were studied in fruits of a somatic hybrid allotetraploid between Willow leaf mandarin (Citrus deliciosa Ten.) + Eureka lemon [Citrus limon (L.) Burm.] and the two diploid parents. The somatic hybrid (WLM + EUR) combined both nuclear genomes of the parents, with chloroplasts and mitochondria of mandarin. Variations in sugar and acid content were studied in fruit pulp during the maturing period, and the chemical composition of peel oils was investigated by capillary gas chromatography (GC), GC/mass spectrometry (MS), and (13)C NMR. The somatic hybrid was close to the lemon parent in the synthesis of organic acids and close to the mandarin parent in fructose content, while sucrose and glucose contents were between the two parents. The aromatic compounds of WLM + EUR were close to mandarin with a non-negligible effect of lemon, which inhibits the methyl N-methylanthranilate, a mandarin-specific compound. Our results lead us to conclude that biosynthesis of compounds involved in citrus fruit quality is not inherited in an additive way in the allotetraploid hybrid. We observed mandarin dominance for fructose and most of the aromatic compounds, lemon dominance for organic acid and methyl N-methylanthranilate, and codominance for sucrose and glucose.

[1]  P. Ollitrault,et al.  New universal mitochondrial PCR markers reveal new information on maternal citrus phylogeny , 2011, Tree Genetics & Genomes.

[2]  吕一旭 Yixu Lu.  引言 (Introduction) , 2009, Provincial China.

[3]  P. Ollitrault,et al.  Influence of mitochondria origin on fruit quality in a citrus cybrid. , 2008, Journal of agricultural and food chemistry.

[4]  J. Forment,et al.  A genome-wide 20 K citrus microarray for gene expression analysis , 2008, BMC Genomics.

[5]  P. Brottier,et al.  Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco) , 2008, Molecular ecology resources.

[6]  S. Lucretti,et al.  Production and characterization of new triploid seedless progenies for mandarin improvement , 2007 .

[7]  L. Abbate,et al.  Polyphenols distribution in juices from Citrus allotetraploid somatic hybrids and their sexual hybrids. , 2007, Journal of agricultural and food chemistry.

[8]  J. Grosser,et al.  Production of mandarin + pummelo somatic hybrid citrus rootstocks with potential for improved tolerance/resistance to sting nematode , 2007 .

[9]  Z. Chen,et al.  Genetic and epigenetic mechanisms for gene expression and phenotypic variation in plant polyploids. , 2007, Annual review of plant biology.

[10]  L. Berti,et al.  Changes in organic acids and sugars during early stages of development of acidic and acidless citrus fruit. , 2006, Journal of agricultural and food chemistry.

[11]  J. Forment,et al.  Global Analysis of Gene Expression During Development and Ripening of Citrus Fruit Flesh. A Proposed Mechanism for Citric Acid Utilization , 2006, Plant Molecular Biology.

[12]  P. Ollitrault,et al.  Citrus somatic allotetraploid hybrids exhibit a differential reduction of leaf sesquiterpenoid biosynthesis compared with their parents , 2005 .

[13]  M. C. de Vicente,et al.  A set of primers for analyzing chloroplast DNA diversity in Citrus and related genera. , 2005, Tree physiology.

[14]  A. Verzera,et al.  Essential oil composition of Citrus meyerii Y. Tan. and Citrus medica L. cv. Diamante and their lemon hybrids. , 2005, Journal of agricultural and food chemistry.

[15]  P. Ollitrault,et al.  Leaf volatile compounds of six citrus somatic allotetraploid hybrids originating from various combinations of lime, lemon, citron, sweet orange, and grapefruit. , 2005, Journal of agricultural and food chemistry.

[16]  J. Glaszmann,et al.  Characterisation of the double genome structure of modern sugarcane cultivars (Saccharum spp.) by molecular cytogenetics , 1996, Molecular and General Genetics MGG.

[17]  A. Ferguson,et al.  Allotetraploid hybrids produced by protoplast fusion for seedless triploid Citrus breeding , 2005, Euphytica.

[18]  H. Yi,et al.  Somatic hybrids between navel orange (Citrus sinensis) and grapefruit (C. paradisi) for seedless triploid breeding , 2000, Euphytica.

[19]  P. Ollitrault,et al.  Leaf volatile compounds of seven citrus somatic tetraploid hybrids sharing willow leaf mandarin (Citrus deliciosa Ten.) as their common parent. , 2003, Journal of agricultural and food chemistry.

[20]  Manolis Vamvakias,et al.  Composition and seasonal variation of the essential oil from leaves and peel of a Cretan lemon variety. , 2002, Journal of agricultural and food chemistry.

[21]  Tomi,et al.  Chemical variability of peel and leaf essential oils of 15 species of mandarins. , 2001, Biochemical Systematics and Ecology.

[22]  P. Ollitrault,et al.  Somatic hybridization in citrus: An effective tool to facilitate variety improvement , 2000, In Vitro Cellular & Developmental Biology - Plant.

[23]  P. Ollitrault,et al.  Somatic hybridisation potential for citrus germplasm utilization , 2000 .

[24]  G. Alonzo,et al.  Citrus somatic hybrid leaf essential oil. , 2000 .

[25]  L. Cohen,et al.  Aconitase activity and expression during the development of lemon fruit , 2000 .

[26]  F. Tomi,et al.  Chemical variability of peel and leaf essential oils of mandarins from Citrus reticulata Blanco , 2000 .

[27]  M. Germanà,et al.  COMPARISON BETWEEN ESSENTIAL OIL YIELD AND CONSTITUENTS OF AN ALLOTETRAPLOID SOMATIC HYBRID OF CITRUS AND ITS PARENTS , 1998 .

[28]  F. Tomi,et al.  Computer-aided identification of individual components of essential oils using Carbon-13 NMR spectroscopy , 1995 .

[29]  P. Ollitrault,et al.  Nuclear genome size variations in Citrus , 1994 .

[30]  R. Adams,et al.  Identification of Essential Oils by Ion trap Mass Spectroscopy , 1989 .

[31]  E. Vangdal,et al.  Quality Criteria for Fruit for Fresh Consumption , 1985 .

[32]  J. Bain.  Morphological, anatomical, and physiological changes in the developing fruit of the Valencia orange, Citrus sinensis (L) Osbeck , 1958 .