Human type III collagen gene expression is coordinately modulated with the type I collagen genes during fibroblast growth.

Type III collagen is one of the major interstitial collagens and, as such, plays an important role in modulating the structure and function of most tissues. To compare the expression of the type III collagen gene to that of the type I collagen alpha 1(I) and alpha 2(I) genes, cDNAs encoding the 3' one-third of the human alpha 1(III) collagen mRNA were obtained by screening a human fetal lung fibroblast cDNA library with a cloned segment of the chicken alpha 1(III) gene. Northern blot analysis of human fetal lung fibroblast RNA demonstrated two alpha 1(III)-specific mRNAs of sizes 6.6 and 5.8 kilobases, sizes clearly different from those of the type I collagen mRNAs. Analyses of populations of dividing and nondividing human lung fibroblasts revealed that, on a per cell basis, the nondividing population contained twice as much alpha 1(III) mRNA than did the dividing population. The same was true for the type I collagen alpha 1(I) and alpha 2(I) mRNA transcripts. Similar results were obtained when alpha 1(III), alpha 1(I), and alpha 2(I) mRNA transcripts were quantified by using dot blot evaluation of total RNA, Northern analysis of total RNA, and dot blot evaluation of cytoplasmic RNA. Thus, despite the fact that the alpha 1(III) collagen gene is located on a chromosome different from the alpha 1(I) and alpha 2(I) genes, the expression of these three collagen chains appears to be coordinately controlled during periods of rapid and slow fibroblast growth.

[1]  R. Crystal,et al.  Control of collagen production by human diploid lung fibroblasts. , 1980, The Journal of biological chemistry.

[2]  M. J. Johnson,et al.  A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322. , 1981, Gene.

[3]  R. Crystal,et al.  Regional localization of the human alpha 2(I) collagen gene on chromosome 7 by molecular hybridization. , 1983, Cytogenetics and cell genetics.

[4]  V. McKusick,et al.  Patients with Ehlers-Danlos syndrome type IV lack type III collagen. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[5]  D. Barlow,et al.  Chromosomal assignments of the genes coding for human types II, III, and IV collagen: a dispersed gene family. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[6]  J. Myers,et al.  Human proα1(I) collagen gene structure reveals evolutionary conservation of a pattern of introns and exons , 1984, Nature.

[7]  M. Di Liberto,et al.  Analysis of the 3' end of the human pro-alpha 2(I) collagen gene. Utilization of multiple polyadenylation sites in cultured fibroblasts. , 1983, The Journal of biological chemistry.

[8]  D. Rowe,et al.  Confirmation that the Type I collagen gene on chromosome 17 is COL1A1 (α1(I)), using a human genomic probe , 1984, Annals of human genetics.

[9]  J. Rosenbloom,et al.  Molecular cloning and carboxyl-propeptide analysis of human type III procollagen. , 1984, Nucleic acids research.

[10]  D. Hanahan,et al.  [24] Plasmid screening at high colony density , 1983 .

[11]  J. Seyer,et al.  Human alpha 1(III) and alpha 2(V) procollagen genes are located on the long arm of chromosome 2. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M. Summers,et al.  The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. , 1980, Analytical biochemistry.

[13]  D. Helinski,et al.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[14]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[15]  D. Ish-Horowicz,et al.  Rapid and efficient cosmid cloning , 1981, Nucleic Acids Res..

[16]  Y. Yamada,et al.  A conserved nucleotide sequence, coding for a segment of the C-propeptide, is found at the same location in different collagen genes. , 1983, Nucleic acids research.

[17]  S. Faro,et al.  Cloning a cDNA for the pro-alpha 2 chain of human type I collagen. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[18]  G. Barsh,et al.  Altered secretion of type III procollagen in a form of type IV Ehlers-Danlos syndrome. Biochemical studies in cultured fibroblasts. , 1981, Laboratory investigation; a journal of technical methods and pathology.

[19]  M. Gross,et al.  Assignment of the human pro alpha 2(I) collagen structural gene (COLIA2) to chromosome 7 by molecular hybridization. , 1982, American journal of human genetics.

[20]  B. Hall,et al.  Sequence of the gene for iso-l-cytochrome c in saccharomyces cerevisiae , 1979, Cell.

[21]  R. Crystal,et al.  Procollagen messenger RNA levels and activity and collagen synthesis during the fetal development of sheep lung, tendon, and skin. , 1981, The Journal of biological chemistry.

[22]  P. Doty,et al.  Procollagen genes: further sequence studies and interspecies comparisons. , 1983, Cold Spring Harbor symposia on quantitative biology.

[23]  R. Timpl,et al.  Type III procollagen is the major collageneous component produced by a continuous rhabdomyosarcoma cell line , 1979, FEBS letters.

[24]  I. Pastan,et al.  Use of recombinant plasmids to characterize collagen RNAs in normal and transformed chick embryo fibroblasts. , 1979, The Journal of biological chemistry.

[25]  J. Vieira,et al.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. , 1982, Gene.

[26]  D. Weil,et al.  Isolation of cDNA and genomic clones encoding human pro-alpha 1 (III) collagen. Partial characterization of the 3' end region of the gene. , 1985, The Journal of biological chemistry.

[27]  R. Crystal,et al.  Rigid control of synthesis of collagen Types I and III by cells in culture , 1977, Nature.

[28]  F. Pope,et al.  Molecular Abnormalities of Collagen: A Review , 1983, Journal of the Royal Society of Medicine.

[29]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[30]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[31]  K. Cheah,et al.  Identification and characterization of the human type II collagen gene (COL2A1). , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[32]  P. Thomas,et al.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[33]  R. Crystal,et al.  Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth. , 1981, The Journal of biological chemistry.

[34]  M. Jaye,et al.  Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX. , 1983, Nucleic acids research.

[35]  K. Cheah,et al.  Isolation and characterization of a human collagen α 1(I)-like gene from a cosmid library , 1982 .

[36]  M. Mudryj,et al.  Isolation and characterization of a genomic clone encoding chick alpha 1 type III collagen. , 1983, The Journal of biological chemistry.

[37]  J. Seyer,et al.  Covalent structure of collagen: amino acid sequence of alpha 1(III)-CB9 from type III collagen of human liver. , 1981, Biochemistry.

[38]  P Berg,et al.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. , 1977, Journal of molecular biology.

[39]  E. J. Miller,et al.  Collagen: an overview. , 1982, Methods in enzymology.

[40]  M. Gross,et al.  Human type I procollagen genes are located on different chromosomes. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[41]  P. Bornstein,et al.  Structurally distinct collagen types. , 1980, Annual review of biochemistry.

[42]  B. White,et al.  Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples. , 1982, The Journal of biological chemistry.

[43]  Douglas L. Brutlag,et al.  SEQ: a nucleotide sequence analysis and recombination system , 1982, Nucleic Acids Res..

[44]  J. Myers,et al.  Cloning and characterization of five overlapping cDNAs specific for the human pro alpha 1(I) collagen chain. , 1982, Nucleic acids research.

[45]  R. Crystal,et al.  Copy number of a human type I alpha 2 collagen gene. , 1982, The Journal of biological chemistry.