论文信息 - The Construction of an Amperometric Immunosensor for the Thyroid Hormone (+)-3,3′,5-Triiodo-L-Thyronine (L-T3)

The Construction of an Amperometric Immunosensor for the Thyroid Hormone (+)-3,3′,5-Triiodo-L-Thyronine (L-T3)

ABSTRACT A novel amperometric immunosensor based on graphite paste (graphite powder and paraffin oil) has been constructed. The graphite paste is impregnated with mouse monoclonal anti-(+)-3,3′,5-triiodo-L-thyronine (anti-T3). The immunosensor can be reliably used for L-T3 assay in thyroid and in drugs, using chronoamperometric technique, at ppb-ppt concentration levels. The potential used for L-T3 assay was 650 mV vs Ag/AgCl. The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new assay.

G. Radu | H. Aboul‐Enein | G. Baiulescu | R. Stefan

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