Characterization of the Molt Stages in Penaeus vannamei: Setogenesis and Hemolymph Levels of Total Protein, Ecdysteroids, and Glucose

The molting cycle of Penaeus vannamei ju veniles was characterized by distinct and predictable changes in the setae of pleopods. The molt pattern was diecdysic with a relatively short intermolt period (40%) and a long proecdysial period (>53%). The levels of both total protein and ecdysteroids increased in the hemo lymph during proecdysis, whereas the level of hemo lymph glucose was low at metecdysis and proecdysis and maximal during anecdysis. As revealed by SDS-PAGE, the relative concentrations of two polypeptides (32 kD; 175 kD) changed during the molting cycle.

[1]  A. Lawrence,et al.  Practical Molt Staging of Penaeus setiferus and Penaeus stylirostris , 1987 .

[2]  J. Delbecque,et al.  Ecdysteroid titers during the molt cycle of Orchestia cavimana (Crustacea, Amphipoda). , 1987, General and comparative endocrinology.

[3]  M. Moriyasu,et al.  Molt Stages of the Snow Crab Chionoecetes Opilio by Observation of Morphogenesis of Setae on the Maxilla , 1986 .

[4]  D. M. Skinner.  2 – Molting and Regeneration , 1985 .

[5]  P. Cassier,et al.  Ecdysteroids in the shrimp Palaemon serratus: relations with molt cycle. , 1984, General and comparative endocrinology.

[6]  E. Longmuir.  Setal development, moult-staging and ecdysis in the banana prawn Penaeus merguiensis , 1983 .

[7]  P. Hopkins.  Patterns of serum ecdysteroids during induced and uninduced proecdysis in the fiddler crab, Uca pugilator. , 1983, General and comparative endocrinology.

[8]  Cynthia Soumoff,et al.  Ecdysteroid titers during the molt cycle of the blue crab resemble those of other Crustacea , 1983 .

[9]  C. Macdonald,et al.  Molt Stage Determination in the Hawaiian Spiny Lobster Panulirus Marginatus , 1983 .

[10]  J. Wriston,et al.  Catabolism of hemocyanin in the American lobster, Homarus americanus , 1981 .

[11]  E. S. Chang,et al.  Ecdysteroid titers of juvenile lobsters following molt induction , 1980 .

[12]  J. McCarthy,et al.  Proecdysial changes in serum ecdysone titers, gastrolith formation, and limb regeneration following molt induction by limb autotomy and/or eyestalk removal in the land crab, Gecarcinus lateralis. , 1977, General and comparative endocrinology.

[13]  E. S. Chang,et al.  The qualitative and quantitative determinations of ecdysones in tissues of the crab, Pachygrapsus crassipes, following molt induction. , 1976, General and comparative endocrinology.

[14]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[15]  W. Dall.  Indices of nutritional state in the western rock lobster, Panulirus longipes (Milne Edwards). I. Blood and tissue constituents and water content , 1974 .

[16]  D. Aiken.  Proecdysis, Setal Development, and Molt Prediction in the American Lobster (Homarus americanus) , 1973 .

[17]  J. R. Stevenson,et al.  Role of acetylglucosamine in chitin synthesis in crayfish. I. Correlation of 14C-acetylglucosamine incorporation with stages of the molting cycle. , 1973, Comparative biochemistry and physiology. B, Comparative biochemistry.

[18]  M. Fingerman,et al.  A female-limited lipoprotein and the diversity of hemocyanin components in the dimorphic variants of the fiddler crab, Uca pugilator, as revealed by disc electrophoresis , 1971 .

[19]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[20]  M. Kerr.  The hemolymph proteins of the blue crab, Callinectes sapidus. II. A lipoprotein serologically identical to oocyte lipovitellin. , 1969, Developmental biology.

[21]  M. Telford.  THE IDENTIFICATION AND MEASUREMENT OF SUGARS IN THE BLOOD OF THREE SPECIES OF ATLANTIC CRABS , 1968 .

[22]  H. Schafer.  The determination of some stages of the molting cycle of Penaeus duorarum, by microscopic examination of the setae of the endopodites of pleopods , 1968 .

[23]  N. Kurup.  THE INTERMOLT CYCLE OF AN ANOMURAN, PETROLISTHES CINCTIPES RANDALL (CRUSTACEA-DECAPODA) , 1964 .

[24]  L. Passano.  CHAPTER 15 – MOLTING AND ITS CONTROL , 1960 .

[25]  B. T. Scheer.  Aspects of the intermoult cycle in natantians , 1960 .

[26]  V. Marks.  An improved glucose-oxidase method for determining blood, C.S.F. and urine glucose levels. , 1959, Clinica chimica acta; international journal of clinical chemistry.

[27]  D. B. Carlisle,et al.  ENDOCRINE CONTROL IN THE CRUSTACEA , 1956 .

[28]  D. Travis.  THE MOLTING CYCLE OF THE SPINY LOBSTER, PANULIRUS ARGUS LATREILLE. II. PRE-ECDYSIAL HISTOLOGICAL AND HISTOCHEMICAL CHANGES IN THE HEPATOPANCREAS AND INTEGUMENTAL TISSUES , 1955 .