Assessment of genetic purity and earliness in F1 and F2 population of cucumber (Cucumis sativus L.) using SSR markers

Simple Sequence Repeat (SSR) markers were used to assess the genetic purity and earliness in F1 and F2 population of cucumber (Cucumis sativus L.) respectively. Experiment was carried out at the Experimental Research Farm of the Department of Vegetable Science, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (HP) during the years 2015 and 2016. Experimental material used in the study comprised of Khira-75, a commercial variety grown in low and mid hills of the state (Himachal Pradesh) which is late in flowering and genotype PI-618860, an inbred line collected from North Central Regional Plant Introduction Station, USA which is early in flowering and tolerates various biotic stresses. For assessment of genetic purity and earliness the parents Khira-75 and PI-618860 were crossed and F1 and F2 population were raised. Genetic purity studies revealed that one marker CSN 160 was observed to be segregating in the parents as well as the population. Further, scoring of earliness in F2 population revealed promising results using 2 co-dominant SSR00262 and SSR17922 markers, which segregated in the parents and population in correlation with the observed phenotypes.

[1]  Ramesh Kumar,et al.  Expression of heterosis and residual heterosis for traits of earliness and yield in cross combinations of cucumber (Cucumis sativus L.) developed by introgression of indigenous and exotic sources , 2021 .

[2]  P. Dash,et al.  Genetics and molecular mapping of gynoecious (F) locus in cucumber (Cucumis sativus L.) , 2019 .

[3]  X. Gu,et al.  Genetic Analysis and QTL Mapping of Fruit Peduncle Length in Cucumber (Cucumis sativus L.) , 2016, PloS one.

[4]  V. Pande,et al.  Assessment of genetic purity in rice (Oryza sativa L.) hybrids using microsatellite markers , 2016, 3 Biotech.

[5]  A. Ipek,et al.  Development of AFLP markers associated with zucchini yellow mosaicvirus resistance in cucumber (Cucumis sativus L.) , 2015 .

[6]  Zhonghua Zhang,et al.  QTL-seq identifies an early flowering QTL located near Flowering Locus T in cucumber , 2014, Theoretical and Applied Genetics.

[7]  Y. Yoshioka,et al.  Identification of quantitative trait loci for downy mildew resistance in cucumber (Cucumis sativus L.) , 2014, Euphytica.

[8]  X. Sheng,et al.  DNA fingerprinting and genetic purity testing of a new broccoli hybrid using SSR markers , 2013 .

[9]  Zhonghua Zhang,et al.  Genetic Diversity and Population Structure of Cucumber (Cucumis sativus L.) , 2012, PloS one.

[10]  X. Sheng,et al.  Genetic purity testing of loose-curd cauliflower hybrids using SSR markers and grow out test , 2012 .

[11]  Yi Ren,et al.  Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai , 2012, Euphytica.

[12]  Zhonghua Zhang,et al.  A linkage map of cultivated cucumber (Cucumis sativus L.) with 248 microsatellite marker loci and seven genes for horticulturally important traits , 2011, Euphytica.

[13]  P. Rajendrakumar,et al.  EST-SSR marker-based assay for the genetic purity assessment of safflower hybrids , 2009, Euphytica.

[14]  Y. Yoshioka,et al.  Development of 101 novel SSR markers and construction of an SSR-based genetic linkage map in cucumber (Cucumis sativus L.) , 2008 .

[15]  B. C. Viraktamath,et al.  Identification of informative SSR markers capable of distinguishing hybrid rice parental lines and their utilization in seed purity assessment , 2008, Euphytica.

[16]  Liwang Liu,et al.  Assessment of genetic purity of tomato (Lycopersicon esculentum L.) hybrid using molecular markers , 2007 .

[17]  Y. Wang,et al.  Seed genetic purity testing of F1 hybrid cabbage (Brassica oleracea var. capitata) with molecular marker analysis , 2007 .

[18]  Tianzhen Zhang,et al.  A Microsatellite-Based, Gene-Rich Linkage Map Reveals Genome Structure, Function and Evolution in Gossypium , 2007, Genetics.

[19]  D. Bulgarelli,et al.  Marker assisted selection in crop plants , 2005, Plant Cell, Tissue and Organ Culture.

[20]  M. Tanurdžić,et al.  Sex-Determining Mechanisms in Land Plants , 2004, The Plant Cell Online.

[21]  Xiefei Zhu,et al.  Inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in Gossypium hirsutum L. , 2003, Theoretical and Applied Genetics.

[22]  R. Sonti,et al.  Assessment of purity of rice hybrids using microsatellite and STS markers , 2002 .

[23]  Zhongxu Lin,et al.  QTL mapping for economic traits based on a dense genetic map of cotton with PCR-based markers using the interspecific cross of Gossypium hirsutum × Gossypium barbadense , 2006, Euphytica.