Improved expansion of equine cord blood derived mesenchymal stromal cells by using microcarriers in stirred suspension bioreactors

[1]  Youshan Melissa Lin,et al.  Critical attributes of human early mesenchymal stromal cell-laden microcarrier constructs for improved chondrogenic differentiation , 2017, Stem Cell Research & Therapy.

[2]  Marta M. Silva,et al.  Bioprocess integration for human mesenchymal stem cells: From up to downstream processing scale-up to cell proteome characterization. , 2017, Journal of biotechnology.

[3]  T. Lawson,et al.  Process development for expansion of human mesenchymal stromal cells in a 50 L single-use stirred tank bioreactor , 2017 .

[4]  A. Lam,et al.  Biodegradable poly-ε-caprolactone microcarriers for efficient production of human mesenchymal stromal cells and secreted cytokines in batch and fed-batch bioreactors. , 2017, Cytotherapy.

[5]  Mutsumi Takagi,et al.  Effects of agitation rate on aggregation during beads-to-beads subcultivation of microcarrier culture of human mesenchymal stem cells , 2017, Cytotechnology.

[6]  Sarah I. M. Lepage,et al.  Generation of equine osteochondral constructs using mesenchymal stromal cells , 2016 .

[7]  A. Lam,et al.  Biodegradable ECM-coated PCL microcarriers support scalable human early MSC expansion and in vivo bone formation. , 2016, Cytotherapy.

[8]  Youshan Melissa Lin,et al.  Expansion in microcarrier-spinner cultures improves the chondrogenic potential of human early mesenchymal stromal cells. , 2016, Cytotherapy.

[9]  T. Ma,et al.  Biomanufacturing of human mesenchymal stem cells in cell therapy: Influence of microenvironment on scalable expansion in bioreactors , 2016 .

[10]  C. Hewitt,et al.  Characterization of human mesenchymal stem cells from multiple donors and the implications for large scale bioprocess development , 2016 .

[11]  C. Hewitt,et al.  Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors , 2016, Biotechnology journal.

[12]  M. Kraume,et al.  Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes , 2016, Stem cells international.

[13]  J. R. Smith,et al.  Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use , 2016, Stem cells international.

[14]  Lanjuan Li,et al.  Energy Metabolism Plays a Critical Role in Stem Cell Maintenance and Differentiation , 2016, International journal of molecular sciences.

[15]  Azza Mostafa Ibrahim,et al.  Chondrogenic differentiation of human umbilical cord blood‐derived mesenchymal stem cells in vitro , 2015 .

[16]  M. Makhlouf,et al.  Chondrogenic differentiation of human umbilical cord blood‐derived mesenchymal stem cells in vitro , 2015, Microscopy research and technique.

[17]  S. Cartmell,et al.  Low‐density subculture: a technical note on the importance of avoiding cell‐to‐cell contact during mesenchymal stromal cell expansion , 2015, Journal of tissue engineering and regenerative medicine.

[18]  Yulin Li,et al.  Feasibility of human hair follicle-derived mesenchymal stem cells/CultiSpher®-G constructs in regenerative medicine , 2015, Cell and Tissue Research.

[19]  D. Bienzle,et al.  Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells , 2015, PloS one.

[20]  Teng Ma,et al.  Metabolic regulation of mesenchymal stem cell in expansion and therapeutic application , 2015, Biotechnology progress.

[21]  Phuc Van Pham,et al.  Production of good manufacturing practice-grade human umbilical cord blood-derived mesenchymal stem cells for therapeutic use. , 2015, Methods in molecular biology.

[22]  L. Amelse,et al.  In vitro analysis of equine, bone marrow-derived mesenchymal stem cells demonstrates differences within age- and gender-matched horses. , 2014, Equine veterinary journal.

[23]  D. Covas,et al.  Growth and functional harvesting of human mesenchymal stromal cells cultured on a microcarrier‐based system , 2014, Biotechnology progress.

[24]  M. S. Kallos,et al.  Improved expansion of human bone marrow‐derived mesenchymal stem cells in microcarrier‐based suspension culture , 2014, Journal of tissue engineering and regenerative medicine.

[25]  M. Vickaryous,et al.  Membrane culture and reduced oxygen tension enhances cartilage matrix formation from equine cord blood mesenchymal stromal cells in vitro. , 2014, Osteoarthritis and cartilage.

[26]  S. Piepers,et al.  Successful isolation of equine mesenchymal stromal cells from cryopreserved umbilical cord blood-derived mononuclear cell fractions. , 2013, Equine veterinary journal.

[27]  M. Choolani,et al.  Microcarrier Culture for Efficient Expansion and Osteogenic Differentiation of Human Fetal Mesenchymal Stem Cells , 2013, BioResearch open access.

[28]  Christopher J. Hewitt,et al.  Scale-up of human mesenchymal stem cell culture: current technologies and future challenges , 2013 .

[29]  K. Braeckmans,et al.  In search for cross‐reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry , 2012, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[30]  J. Dudhia,et al.  Implantation of bone marrow-derived mesenchymal stem cells demonstrates improved outcome in horses with overstrain injury of the superficial digital flexor tendon. , 2012, Equine veterinary journal.

[31]  I. Martín-Burriel,et al.  Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue. , 2011, Veterinary immunology and immunopathology.

[32]  Joost D de Bruijn,et al.  The metabolism of human mesenchymal stem cells during proliferation and differentiation , 2011, Journal of cellular physiology.

[33]  J. Gimble,et al.  Toward a clinical-grade expansion of mesenchymal stem cells from human sources: a microcarrier-based culture system under xeno-free conditions. , 2011, Tissue engineering. Part C, Methods.

[34]  Robert J. Thomas,et al.  Expansion of human mesenchymal stem cells on microcarriers , 2011, Biotechnology Letters.

[35]  J. R. Sharpe,et al.  Microcarriers and their potential in tissue regeneration. , 2011, Tissue engineering. Part B, Reviews.

[36]  A. Aguzzi,et al.  Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture , 2010, PloS one.

[37]  L.Y. Sun,et al.  Cell proliferation of human bone marrow mesenchymal stem cells on biodegradable microcarriers enhances in vitro differentiation potential , 2010, Cell proliferation.

[38]  Jeremy J Mao,et al.  Shear stress induces osteogenic differentiation of human mesenchymal stem cells. , 2010, Regenerative medicine.

[39]  Byung-Soo Kim,et al.  Suspension Culture of Mammalian Cells Using Thermosensitive Microcarrier that Allows Cell Detachment without Proteolytic Enzyme Treatment , 2010, Cell transplantation.

[40]  Y. Okada,et al.  Basic fibroblast growth factor in the bone microenvironment enhances cell motility and invasion of Ewing's sarcoma family of tumours by activating the FGFR1–PI3K–Rac1 pathway , 2010, British Journal of Cancer.

[41]  José Eduardo Krieger,et al.  Shear stress induces nitric oxide-mediated vascular endothelial growth factor production in human adipose tissue mesenchymal stem cells. , 2010, Stem cells and development.

[42]  H. Prins,et al.  Expansion of human mesenchymal stromal cells on microcarriers: growth and metabolism , 2010, Journal of tissue engineering and regenerative medicine.

[43]  P. Thomsen,et al.  Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells. , 2009, Cytotherapy.

[44]  M. Korc,et al.  The role of fibroblast growth factors in tumor growth. , 2009, Current cancer drug targets.

[45]  Joost D de Bruijn,et al.  Growth, metabolism, and growth inhibitors of mesenchymal stem cells. , 2009, Tissue engineering. Part A.

[46]  K. McCloskey,et al.  Can shear stress direct stem cell fate? , 2009, Biotechnology progress.

[47]  Roger K. Smith.  Mesenchymal stem cell therapy for equine tendinopathy , 2008, Disability and rehabilitation.

[48]  P. Czermak,et al.  Expansion and Harvesting of hMSC-TERT , 2007, The open biomedical engineering journal.

[49]  J. Frangos,et al.  The shear stress of it all: the cell membrane and mechanochemical transduction , 2007, Philosophical Transactions of the Royal Society B: Biological Sciences.

[50]  P. Thomsen,et al.  Isolation of mesenchymal stem cells from equine umbilical cord blood , 2007, BMC biotechnology.

[51]  Ardeshir Ghavamzadeh,et al.  BMC Cell Biology BioMed Central Research article Aging of mesenchymal stem cell in vitro , 2005 .

[52]  Peter W Zandstra,et al.  Shear‐Controlled Single‐Step Mouse Embryonic Stem Cell Expansion and Embryoid Body–Based Differentiation , 2005, Stem cells.

[53]  S. Dyson,et al.  Medical management of superficial digital flexor tendonitis: a comparative study in 219 horses (1992-2000). , 2010, Equine veterinary journal.

[54]  Y. Kato,et al.  Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. , 2001, Biochemical and biophysical research communications.

[55]  D J Prockop,et al.  Donor variation in the growth properties and osteogenic potential of human marrow stromal cells , 1999, Journal of cellular biochemistry.