Direct Reverse Transcription Real-Time PCR of Viral RNA from Saliva Samples Using Hydrogel Microparticles

[1]  S. Sim,et al.  Highly sensitive and multiplexed one-step RT-qPCR for profiling genes involved in the circadian rhythm using microparticles , 2021, Scientific Reports.

[2]  J. Zimmerman,et al.  Guidelines for oral fluid-based surveillance of viral pathogens in swine , 2020, Porcine Health Management.

[3]  Petr Kvapil,et al.  Direct-RT-qPCR Detection of SARS-CoV-2 without RNA Extraction as Part of a COVID-19 Testing Strategy: From Sample to Result in One Hour , 2020, Diagnostics.

[4]  A. Fomsgaard,et al.  An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020 , 2020, medRxiv.

[5]  L. Lovat,et al.  An optimised saliva collection method to produce high-yield, high-quality RNA for translational research , 2020, PloS one.

[6]  Yuanming Li,et al.  A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor. , 2020, The Analyst.

[7]  Antonino Bella,et al.  Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing , 2020, Emerging microbes & infections.

[8]  S. Kong,et al.  Development of a Direct-Reverse Transcription-Quantitative PCR (dirRT-qPCR) assay for Clinical Zika Diagnosis. , 2019, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[9]  A. Acevedo,et al.  Impact of RNA Degradation on Viral Diagnosis: An Understated but Essential Step for the Successful Establishment of a Diagnosis Network , 2018, Veterinary sciences.

[10]  Tania Nolan,et al.  Talking the talk, but not walking the walk: RT‐qPCR as a paradigm for the lack of reproducibility in molecular research , 2017, European journal of clinical investigation.

[11]  J. Macdonald,et al.  DNA Heat Treatment for Improving qPCR Analysis of Human Adenovirus in Wastewater , 2017, Food and Environmental Virology.

[12]  E. H. Oh,et al.  Microparticle-based RT-qPCR for highly selective rare mutation detection. , 2017, Biosensors & bioelectronics.

[13]  Nakwon Choi,et al.  Extensible Multiplex Real-time PCR of MicroRNA Using Microparticles , 2016, Scientific Reports.

[14]  Yinglin Xia,et al.  TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR , 2015, PloS one.

[15]  Zhi-Luo Deng,et al.  Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation , 2015, BMC Genomics.

[16]  Keli Yang,et al.  A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification , 2014, Journal of Animal Science and Biotechnology.

[17]  Yong-xiang Tian,et al.  A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification , 2014, Journal of Animal Science and Biotechnology.

[18]  P. Celec,et al.  On the origin and diagnostic use of salivary RNA. , 2014, Oral diseases.

[19]  I. Decorte,et al.  Effect of saliva stabilisers on detection of porcine reproductive and respiratory syndrome virus in oral fluid by quantitative reverse transcriptase real-time PCR. , 2013, Veterinary journal.

[20]  S. K. Li,et al.  Characterization of human sclera barrier properties for transscleral delivery of bevacizumab and ranibizumab. , 2013, Journal of pharmaceutical sciences.

[21]  C. Schrader,et al.  PCR inhibitors – occurrence, properties and removal , 2012, Journal of applied microbiology.

[22]  M. Navazesh Saliva in Health and Disease: The Present and Future of a Unique Sample for Diagnosis , 2011, Journal of the California Dental Association.

[23]  D. Patnayak,et al.  Detection of Influenza a Virus in Porcine Oral Fluid Samples , 2011, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[24]  K. Harmon,et al.  Comparison of RNA Extraction and Real-Time Reverse Transcription Polymerase Chain Reaction Methods for the Detection of Porcine Reproductive and Respiratory Syndrome Virus in Porcine Oral Fluid Specimens , 2011, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[25]  M. Robitaille,et al.  Ambient temperature storage of RNA in GenTegra™ for use in RT-qPCR , 2010 .

[26]  S. Tan,et al.  DNA, RNA, and Protein Extraction: The Past and The Present , 2009, Journal of biomedicine & biotechnology.

[27]  H. Poinar,et al.  A quantitative approach to detect and overcome PCR inhibition in ancient DNA extracts. , 2009, BioTechniques.

[28]  P. Doyle,et al.  Optimization of encoded hydrogel particles for nucleic acid quantification. , 2009, Analytical chemistry.

[29]  S. Blanc,et al.  A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses , 2008, Virology Journal.

[30]  N. Park,et al.  RNAprotect saliva: An optimal room- temperature stabilization reagent for the salivary transcriptome. , 2006, Clinical chemistry.

[31]  N. Park,et al.  Characterization of RNA in Saliva , 2006, Clinical chemistry.

[32]  V. Chechetkin,et al.  Discrimination Between Perfect and Mismatched Duplexes with Oligonucleotide Gel Microchips: Role of Thermodynamic and Kinetic Effects During Hybridization , 2005, Journal of biomolecular structure & dynamics.

[33]  Tania Nolan,et al.  Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. , 2004, Journal of biomolecular techniques : JBT.

[34]  N. Johnson,et al.  Inhibitory effect of salivary fluids on PCR: potency and removal. , 1994, PCR methods and applications.

[35]  D. Holmes,et al.  A rapid boiling method for the preparation of bacterial plasmids. , 1981, Analytical biochemistry.

[36]  Harry Jerome Production , 1929, American Journal of Sociology.

[37]  Bruce Budowle,et al.  Direct PCR amplification of DNA from human bloodstains, saliva, and touch samples collected with microFLOQ® swabs. , 2018, Forensic science international. Genetics.

[38]  M. Gadanho,et al.  DNA extraction: finding the most suitable method , 2016 .

[39]  D. Wong Salivary Diagnostics: Amazing as it might seem, doctors can detect and monitor diseases using molecules found in a sample of spit. , 2008, American scientist.

[40]  Ning Jiang,et al.  Extremely Rapid Extraction of DNA from Bacteria and Yeasts , 2005, Biotechnology Letters.

[41]  J. Zimmerman,et al.  Journal of Swine Health and Production Blockin— Oral-fluid Blockinsamples Blockinfor Blockinsurveillance Blockinof Blockincommercial Blockingrowing Pigs Blockinfor Blockinporcine Blockinreproductive Blockinand Blockinrespiratory Blockinsyndrome Blockinvirus and Blockinporcine Blockincircovirus Block , 2022 .