Expression of γδTCR on myeloid cells of Plasmodium yoelii nigeriensis -infected C57BL/6 mice

Background: The role of CD3 + γδTCR + (γδT) cells during the Plasmodium yoelii nigeriensis infection has been reported in our previous studies. However, there is a paucity of studies on the characteristics of myeloid cells which expressed γδTCR. Therefore, the aim of this study was to observe the properties of γδTCR-expressing myeloid cells in the spleen of C57BL/6 mice infected by P. yoelii NSM. Methods: Haematoxylin-eosin (HE) staining was used to observe the pathological changes in the spleens. The samples from the experimental group before and after infection underwent RNA sequencing (RNA-seq), and the differentially expressed genes (DEGs) were screened. Flow cytometry (FCM) was used to evaluate the frequency of γδTCR + cells and the characteristics of γδTCR + cells in Plasmodium yoelii-infected mice. Results: We observed obvious in�ltration of in�ammatory in infected C57BL/6 mouse spleens. The proportions of γδTCR + cells and CD11b + γδTCR + cells from infected group were higher than that from normal group. γδTCR + CD11b + cells expressed high levels of activated-mediated genes and in�ammatory-mediated genes. We characterized the heterogeneous pathway activities among γδTCR + CD11b + cells from normal and infected group. The oxidative phosphorylation, respiratory electron transport chain and leukocyte activation involved in immune response pathways were up-regulated, while the alpha-beta T cell activation and myeloid leukocyte migration pathways were down-regulated. Importantly, we found that Ly6c2 was higher expressed in γδTCR + CD11b + cells than Ly6g. Consistent with it, �ow cytometry results revealed that a subset of Ly6C + Ly6G - CD11b + γδTCR + cells was higher than Ly6G + Ly6C - CD11b + γδTCR + cells in the spleen. Conclusions: Taken together, our data suggest the existence of a population of γδTCR-expressing myeloid cells and played potential role in the context of Plasmodium infection. The molecular markers and pathways screened warrant further study.

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