Compact clinical high-NA multiphoton endoscopy

Multiphoton imaging methods are excellent for non-invasive imaging of living tissue without any need of additional contrast agents. The increasing demand for endoscopic techniques has forced the development of multiphoton endoscopes for imaging of areas with reduced accessibility like chronic wounds. Gradient index (GRIN) lenses can miniaturize the bulky distal focusing optics of conventional tomographs to a diameter of less than 1.4 mm and a numerical aperture (NA) of 0.8. We combined a high NA clinical multiphoton endoscope with existing multiphoton tomographs like the DermaInspect® and the MPTflex® to enable the examination of wound healing processes.

[1]  K. König,et al.  Two-photon microscopes and in vivo multiphoton tomographs--powerful diagnostic tools for tissue engineering and drug delivery. , 2006, Advanced drug delivery reviews.

[2]  Iris Riemann,et al.  High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. , 2003, Journal of biomedical optics.

[3]  K. König,et al.  Multiphoton tissue imaging using high‐NA microendoscopes and flexible scan heads for clinical studies and small animal research , 2008, Journal of biophotonics.

[4]  Hans Georg Breunig,et al.  Multiphoton excitation characteristics of cellular fluorophores of human skin in vivo. , 2010, Optics express.

[5]  Karsten König,et al.  Multiphoton microscopy for the investigation of dermal penetration of nanoparticle-borne drugs. , 2006, The Journal of investigative dermatology.

[6]  Karsten König,et al.  Clinical multiphoton tomography and clinical two-photon microendoscopy , 2009, BiOS.

[7]  I. Riemann,et al.  In vivo Drug Screening in Human Skin Using Femtosecond Laser Multiphoton Tomography , 2006, Skin Pharmacology and Physiology.

[8]  K. Fujita.  [Two-photon laser scanning fluorescence microscopy]. , 2007, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.

[9]  Karsten König,et al.  Sensitivity and specificity of multiphoton laser tomography for in vivo and ex vivo diagnosis of malignant melanoma. , 2009, The Journal of investigative dermatology.

[10]  Watt W Webb,et al.  Interpreting second-harmonic generation images of collagen I fibrils. , 2005, Biophysical journal.

[11]  Karsten König,et al.  In vivo assessment of human skin aging by multiphoton laser scanning tomography. , 2006, Optics letters.