论文信息 - The effect of freezing, thawing, and short- and long-term storage on serum thyrotropin, thyroid hormones, and thyroid autoantibodies: implications for analyzing samples stored in serum banks.

The effect of freezing, thawing, and short- and long-term storage on serum thyrotropin, thyroid hormones, and thyroid autoantibodies: implications for analyzing samples stored in serum banks.

Data concerning the effects of freezing, thawing, and storage on serum thyroid-stimulating hormone (TSH) and thyroid hormones [free thyroxine (fT4) and free triiodothyronine (fT3)] are scarce (1)(2)(3), and we can find no data on their effects on thyroid autoantibodies [thyroid-peroxidase antibody (TPO-Ab) and thyroglobulin antibody (TG-Ab)]. We evaluated the effect of freezing, thawing, and storage of samples for up to 23 years on TSH, fT4, fT3, TPO-Ab, and TG-Ab concentrations in human serum (Architect i2000, Abbott Diagnostics). Whole blood samples for the Finnish Maternity Cohort (FMC) were collected without preservative from pregnant women, most of whom were in the 1st trimester of pregnancy. Each sample was stored in a single serum aliquot in a polypropylene cryovial (Nunc GmbH & Co.) at −25 °C. The ethics boards of the Finnish National Public Health Institute and Oulu University Hospital approved this study. The effect of freezing and thawing was evaluated by comparing frozen …

[1] G. Koliakos,et al. Stability of serum TSH concentration after non refrigerated storage. , 1999, Minerva endocrinologica.

[2] K. Ichihara,et al. The stability of immunological and biological activity of human thyrotropin in buffer: its temperature-dependent dissociation into subunits during freezing. , 1991, Scandinavian journal of clinical and laboratory investigation.

[3] D. Fisher,et al. Variation in values for iodothyronine hormones, thyrotropin, and thyroxine-binding globulin in normal umbilical-cord serum with season and duration of storage. , 1979, Clinical chemistry.