A novel human DnaJ protein, hTid-1, a homolog of the Drosophila tumor suppressor protein Tid56, can interact with the human papillomavirus type 16 E7 oncoprotein.

We have cloned hTid-1, a human homolog of the Drosophila tumor suppressor protein Tid56, by virtue of its ability to form complexes with the human papillomavirus E7 oncoprotein. The carboxyl terminal cysteine-rich metal binding domain of E7 is the major determinant for interaction with hTid-1. The carboxyl terminus of E7 is essential for the functional and structural integrity of E7 and has previously been shown to function as a multimerization domain. The hTid-1 protein is a member of the DnaJ-family of chaperones. Its mRNA is widely expressed in human tissues, including the HPV-18-positive cervical carcinoma cell line HeLa and human genital keratinocytes, the normal host cells of the HPVs. The hTid-1 gene has been mapped to the short arm of chromosome 16. The large tumor antigens of polyomaviruses encode functional J-domains that are important for viral replication as well as cellular transformation. The ability of HPV E7 to interact with a cellular DnaJ protein suggests that these two viral oncoproteins may target common regulatory pathways through J-domains.

[1]  J. Pipas,et al.  Polyomavirus T Antigens: Molecular Chaperones for Multiprotein Complexes , 1998, Journal of Virology.

[2]  M. Imperiale,et al.  Novel Mechanisms of E2F Induction by BK Virus Large-T Antigen: Requirement of Both the pRb-Binding and the J Domains , 1998, Molecular and Cellular Biology.

[3]  J. Decaprio,et al.  The J Domain of Simian Virus 40 Large T Antigen Is Required To Functionally Inactivate RB Family Proteins , 1998, Molecular and Cellular Biology.

[4]  K. Münger,et al.  Destabilization of the RB tumor suppressor protein and stabilization of p53 contribute to HPV type 16 E7-induced apoptosis. , 1997, Virology.

[5]  J. Decaprio,et al.  The DnaJ domain of polyomavirus large T antigen is required to regulate Rb family tumor suppressor function , 1997, Journal of virology.

[6]  S. Bagchi,et al.  The Human Papillomavirus E7 Oncoprotein Functionally Interacts with the S4 Subunit of the 26 S Proteasome* , 1997, The Journal of Biological Chemistry.

[7]  M Fujita,et al.  Binding of high-risk human papillomavirus E6 oncoproteins to the human homologue of the Drosophila discs large tumor suppressor protein. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[8]  J. Decaprio,et al.  Inactivation of pRB-related proteins p130 and p107 mediated by the J domain of simian virus 40 large T antigen , 1997, Molecular and cellular biology.

[9]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[10]  K. Münger,et al.  The human papillomavirus E7 oncoprotein can uncouple cellular differentiation and proliferation in human keratinocytes by abrogating p21Cip1-mediated inhibition of cdk2. , 1997, Genes & development.

[11]  B. Stillman,et al.  Inhibition of CDK activity and PCNA-dependent DNA replication by p21 is blocked by interaction with the HPV-16 E7 oncoprotein. , 1997, Genes & development.

[12]  I. M. Marks,et al.  The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain , 1997, Molecular and cellular biology.

[13]  S. S. Lee,et al.  Binding of human virus oncoproteins to hDlg/SAP97, a mammalian homolog of the Drosophila discs large tumor suppressor protein. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[14]  P. Polakis.  The adenomatous polyposis coli (APC) tumor suppressor. , 1997, Biochimica et biophysica acta.

[15]  P. Silver,et al.  DnaJ/hsp40 chaperone domain of SV40 large T antigen promotes efficient viral DNA replication. , 1997, Genes & development.

[16]  C. Georgopoulos,et al.  The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[17]  K. Münger,et al.  Analysis of the p53-mediated G1 growth arrest pathway in cells expressing the human papillomavirus type 16 E7 oncoprotein , 1997, Journal of virology.

[18]  K. Vousden,et al.  Analysis of the interaction between human papillomavirus type 16 E7 and the TATA-binding protein, TBP. , 1997, The Journal of general virology.

[19]  W. Zwerschke,et al.  Inactivation of the cdk inhibitor p27KIP1 by the human papillomavirus type 16 E7 oncoprotein. , 1996, Oncogene.

[20]  K. Münger,et al.  Interactions of the human papillomavirus E7 protein with cell cycle regulators. , 1996, Seminars in cancer biology.

[21]  D. Wazer,et al.  E7 protein of human papilloma virus-16 induces degradation of retinoblastoma protein through the ubiquitin-proteasome pathway. , 1996, Cancer research.

[22]  Harald zur Hausen,et al.  Papillomavirus infections — a major cause of human cancers , 1996 .

[23]  M. Vidal,et al.  Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[24]  A. Goldberg,et al.  Involvement of the molecular chaperone Ydj1 in the ubiquitin-dependent degradation of short-lived and abnormal proteins in Saccharomyces cerevisiae , 1996, Molecular and cellular biology.

[25]  D. Pim,et al.  HPV-16 E7 and adenovirus E1a complex formation with TATA box binding protein is enhanced by casein kinase II phosphorylation. , 1996, Oncogene.

[26]  T. Akiyama,et al.  Binding of APC to the Human Homolog of the Drosophila Discs Large Tumor Suppressor Protein , 1996, Science.

[27]  J. Decaprio,et al.  Simian virus 40 large T antigen alters the phosphorylation state of the RB-related proteins p130 and p107 , 1996, Journal of Virology.

[28]  M. Douglas,et al.  A Conserved HPD Sequence of the J-domain Is Necessary for YDJ1 Stimulation of Hsp70 ATPase Activity at a Site Distinct from Substrate Binding (*) , 1996, The Journal of Biological Chemistry.

[29]  R. Prince.  Photosynthesis: the Z-scheme revised. , 1996, Trends in biochemical sciences.

[30]  J. Brodsky,et al.  Post-translational protein translocation: not all hsc70s are created equal. , 1996, Trends in biochemical sciences.

[31]  M. Birrer,et al.  The human papillomavirus type 16 E7 gene product interacts with and trans‐activates the AP1 family of transcription factors. , 1996, The EMBO journal.

[32]  W. Zwerschke,et al.  Identification of domains required for transcriptional activation and protein dimerization in the human papillomavirus type-16 E7 protein. , 1996, Oncogene.

[33]  L. Laimins,et al.  Human papillomavirus E7 oncoproteins bind a single form of cyclin E in a complex with cdk2 and p107. , 1996, Virology.

[34]  R. Brent,et al.  Dimerization of the human papillomavirus E7 oncoprotein in vivo. , 1995, Virology.

[35]  J. Mazzarelli,et al.  The viral oncoproteins Ad5 E1A, HPV16 E7 and SV40 TAg bind a common region of the TBP-associated factor-110. , 1995, Oncogene.

[36]  T. R. Broker,et al.  Differentiation-dependent up-regulation of the human papillomavirus E7 gene reactivates cellular DNA replication in suprabasal differentiated keratinocytes. , 1995, Genes & development.

[37]  Shuji Takahashi,et al.  70-kDa Heat Shock Cognate Protein Interacts Directly with the N-terminal Region of the Retinoblastoma Gene Product pRb , 1995, The Journal of Biological Chemistry.

[38]  R. Weinberg,et al.  E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Y. Shaul,et al.  The X protein of hepatitis B virus coactivates potent activation domains , 1995, Molecular and cellular biology.

[40]  D. Gundacker,et al.  Tumor suppression in Drosophila is causally related to the function of the lethal(2) tumorous imaginal discs gene, a dnaJ homolog. , 1995, Developmental genetics.

[41]  E. Gateff,et al.  Tumor suppressor and overgrowth suppressor genes of Drosophila melanogaster: developmental aspects. , 1994, The International journal of developmental biology.

[42]  S. Landry,et al.  Chaperone power in a virus? , 1994, Trends in biochemical sciences.

[43]  T. Langer,et al.  DnaJ-like proteins: molecular chaperones and specific regulators of Hsp70. , 1994, Trends in biochemical sciences.

[44]  D. Heimbrook,et al.  Identification of a novel retinoblastoma gene product binding site on human papillomavirus type 16 E7 protein. , 1994, The Journal of biological chemistry.

[45]  S. Thomas,et al.  Identification and characterization of the tuberous sclerosis gene on chromosome 16 , 1993, Cell.

[46]  K. Ohtsuka,et al.  Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ. , 1993, Biochemical and biophysical research communications.

[47]  William J. Welch,et al.  ATP-induced protein Hsp70 complex dissociation requires K+ but not ATP hydrolysis , 1993, Nature.

[48]  P. Raychaudhuri,et al.  Association of the human papillomavirus type 16 E7 protein with the S-phase-specific E2F-cyclin A complex , 1993, Molecular and cellular biology.

[49]  T. Mohanakumar,et al.  Cloning of a unique human homologue of the Escherichia coli DNAJ heat shock protein. , 1993, Biochimica et biophysica acta.

[50]  A. Iwahori,et al.  Human cDNA encoding DnaJ protein homologue. , 1993, Biochimica et biophysica acta.

[51]  P. Silver,et al.  Eukaryotic DnaJ homologs and the specificity of Hsp70 activity , 1993, Cell.

[52]  J. Kvedar,et al.  Epithelial cornified envelope precursors are in the hair follicle and nail. , 1993, The Journal of investigative dermatology.

[53]  S. Grossman,et al.  Human papillomavirus type 18 E7 protein requires intact Cys-X-X-Cys motifs for zinc binding, dimerization, and transformation but not for Rb binding , 1993, Journal of virology.

[54]  K. Münger,et al.  The human papillomavirus E7 oncoprotein and the cellular transcription factor E2F bind to separate sites on the retinoblastoma tumor suppressor protein , 1993, Journal of virology.

[55]  M. Ivey-Hoyle,et al.  Papillomavirus E7 protein binding to the retinoblastoma protein is not required for viral induction of warts , 1993, Journal of virology.

[56]  T. Hunt,et al.  HPV16 E7 protein associates with the protein kinase p33CDK2 and cyclin A. , 1993, Oncogene.

[57]  C. Georgopoulos,et al.  Role of the major heat shock proteins as molecular chaperones. , 1993, Annual review of cell biology.

[58]  K. Münger,et al.  Homologous sequences in adenovirus E1A and human papillomavirus E7 proteins mediate interaction with the same set of cellular proteins , 1992, Journal of virology.

[59]  A. Gown,et al.  Expression of the HPV16 E7 gene generates proliferation in stratified squamous cell cultures which is independent of endogenous p53 levels. , 1992, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.

[60]  D. Gundacker,et al.  Genetic, cytogenetic and developmental analysis of the Drosophila melanogaster tumor suppressor gene lethal(2)tumorous imaginal discs (1(2)tid). , 1992, Differentiation; research in biological diversity.

[61]  E. Fearon,et al.  Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[62]  M. Cheetham,et al.  Human homologues of the bacterial heat-shock protein DnaJ are preferentially expressed in neurons. , 1992, The Biochemical journal.

[63]  K. Münger,et al.  Structure-function analysis of the human papillomavirus type 16 E7 oncoprotein , 1992, Journal of virology.

[64]  D. McCance,et al.  Regions of human papillomavirus type 16 E7 oncoprotein required for immortalization of human keratinocytes , 1992, Journal of virology.

[65]  J. Sambrook,et al.  Protein folding in the cell , 1992, Nature.

[66]  J. Manley,et al.  A human homologue of the Escherichia coli DnaJ heat-shock protein. , 1991, Nucleic acids research.

[67]  C. Georgopoulos,et al.  Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[68]  David M. Livingston,et al.  The product of the retinoblastoma susceptibility gene has properties of a cell cycle regulatory element , 1989, Cell.

[69]  Jun Ma,et al.  GAL4-VP16 is an unusually potent transcriptional activator , 1988, Nature.

[70]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.