Modeling of quantitative microinjection and adaptive control

Aiming at the requirements of the controllability and accuracy of microinjection volume in the cell microinjection technique with the micromanipulator, the lumped model of microinjection pipette is proposed, and based on this model, the adaptive control of microinjection mass flow is designed. Starting from the experimental results, the lumped model from electric circuit was borrowed, which describe the relationship between microinjection mass flow and pressure. Through the analysis of variation of each parameter of Lumped model, the multiple forms of the lumped model is obtained and verified. Because of the combined effect of many factors, it is difficult to obtain the specific values of parameters before the experiment. The adaptive control is used to parameter estimation and tracking the microinjection mass flow. At the end of this paper, the controller is used by matlab simulink to simulation and exactly control of injection volume.