Anthocyanin pigments in the skin of lychee fruit

The anthocyanins in the skin of lychee fruit were isolated and purified by Sephadex G-25 and LH-20 gel column chromatography, and Sep-Pak C-18 cartridge. Further separation by HPLC on a C-18 column, on-line spectra by photodiode array detection, and acid and alkali hydrolyses were used for identification. Cyanidin-3-rutinoside was the major anthocyanin found. Cyanidin-3-gIucoside and malvidin-3-ace-tylglucoside were also identified. Polymerized anthocyanin pigment was also present, appearing to contribute to the brownish-red color.

[1]  B. Barritt,et al.  Cellulose thin-layer chromatographi separation of Rubus fruit anthocyanins , 1973 .

[2]  Marguerite M. Wilkinson,et al.  High-pressure liquid chromatography of anthocyanidins , 1977 .

[3]  F. J. Francis,et al.  Anthocyanins in the Lowbush Blueberry, Vaccinium angustifolium , 1966 .

[4]  F. J. Francis,et al.  ANTHOCYANINS OF ORNAMENTAL CHERRIES , 1975 .

[5]  C. F. Timberlake,et al.  The distribution of anthocyanins in grape skin extracts of port wine cultivars as determined by high performance liquid chromatography , 1985 .

[6]  C. Nagel,et al.  High-Pressure Liquid Chromatographic Separation of Anthocyanins of Vitis Vinifera , 1978, American Journal of Enology and Viticulture.

[7]  J. H. Elbe,et al.  Hydrochloric Acid in Isolating Anthocyanin Pigments from Montmorency Cherries , 1968 .

[8]  T. Somers.  Wine Tannins—Isolation of Condensed Flavonoid Pigments by Gel-Filtration , 1966, Nature.

[9]  T. Somers.  Resolution and analysis of total phenolic constituents of grape pigment , 1967 .

[10]  R. Wrolstad,et al.  Cranberry Juice Composition , 1986 .

[11]  C. F. Timberlake,et al.  Cyanidin 3-malonylglucoside in Cichorium intybus , 1984 .

[12]  J. Harborne.  Plant polyphenols—XI. , 1964 .

[13]  R. Wilson,et al.  The separation of condensed tannins on Sephadex G-25 eluted with 50% aqueous acetone , 1972 .

[14]  G. Avigad.  Dansyl hydrazine as a fluorimetric reagent for thin-layer chromatographic analysis of reducing sugars. , 1977, Journal of chromatography.

[15]  G. Hunter,et al.  Volatile constituents of litchi (Litchi chinesis Sonn.) , 1980 .