Microfiltration cell‐recycle pilot system for continuous thermoanaerobic production of exo‐β‐amylase

A microfiltration cell‐recycle pilot‐scale system was developed comprised of a conventional continuous‐flow fermentor connected to an in situ steam‐sterilizable cross‐flow ceramic filter with a backflushing device. A microcomputer was used to control filtration pressure, tangential flow velocity, and backflushing. Performance of the system was tested with the anaerobic production of thermostable extracellular β‐amylase at 60°C by Clostridium thermosulfurogenes on maltose or malto‐dextrin media. Filtration rates during continuous cultivation were between 20 and 60 L/m2/h. The maltodextrin and cell debris occurring at high retentate flow rates or filtration pressures impaired the performance of the filter. Backflushing initially improved the permeate flux to 42% in a maltose medium and to 10% in a maltodextrin medium, but the effect diminished with time. The productivity of β‐amylase (as much as 48 U/mL/h) and concentration of biomass (as much as 14 g/L) were increased 11‐ and 12‐fold, respectively, if compared to values obtained in a chemostat. The concentration of β‐amylase rose to 220 U/mL in the reactor, which was 5.5‐fold more than under comparable conditions in a chemostat.

[1]  J. Zeikus,et al.  Continuous Production of Thermostable β-Amylase with Clostridium thermosulfurogenes: Effect of Culture Conditions and Metabolite Levels on Enzyme Synthesis and Activity , 1989, Applied and environmental microbiology.

[2]  K. Kroner,et al.  Improved Dynamic Filtration of Microbial Suspensions , 1987, Bio/Technology.

[3]  H. Chang,et al.  Kinetics of ethanol fermentations in membrane cell recycle fermentors , 1987, Biotechnology and bioengineering.

[4]  A. Fiechter,et al.  Performance of a filter loop reactor using Zymomonas mobilis and validation of the ethanol production model — II , 1986 .

[5]  M. Cheryan,et al.  Lactic acid from acid whey permeate in a membrane recycle bioreactor , 1986 .

[6]  G. Goma,et al.  Acetonobutylic fermentation: Improvement of performances by coupling continuous fermentation and ultrafiltration , 1986, Biotechnology and bioengineering.

[7]  J. Zeikus,et al.  Regulation and genetic enhancement of beta-amylase production in Clostridium thermosulfurogenes , 1985, Journal of bacteriology.

[8]  A. Fane,et al.  Flux decline in protein ultrafiltration , 1984 .

[9]  P. Ward,et al.  The separation of aryl acylamidase by cross flow microfiltration and the significance of enzyme/cell debris interaction☆ , 1984 .

[10]  P. Gerhardt,et al.  Microfiltration Culture Process for Enhanced Production of rDNA Receptor Cells of Escherichia Coli , 1984, Bio/Technology.

[11]  P. Gerhardt,et al.  Dialysis continuous process for ammonium lactate fermentation: Simulated prefermentor and cell‐recycling systems , 1981 .

[12]  M. D. Young,et al.  Enhanced product formation in continuous fermentations with microbial cell recycle * , 1981 .

[13]  Kanji Matsumoto,et al.  Separation of yeast by cross-flow filtration with backwashing , 1987 .

[14]  T. Scheper,et al.  Use of membranes to improve bioreactor performance , 1987 .

[15]  Horst Chmiel,et al.  Membranen in der Verfahrenstechnik , 1985 .

[16]  J. Hanemaaijer.  Microfiltration in whey processing , 1985 .

[17]  S. Ripperger,et al.  Crossflow microfiltration in the process industry , 1985 .

[18]  H. Hustedt,et al.  Cross-flow filtration in the downstream processing of enzymes , 1984 .

[19]  B. Breslau,et al.  Advances in Hollow Fiber Ultrafiltration Technology , 1980 .

[20]  P. Gerhardt,et al.  Dialysis culture of microorganisms: design, theory, and results. , 1969, Bacteriological reviews.

[21]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[22]  P. Bernfeld,et al.  [17] Amylases, α and β , 1955 .