Fluorescent fusion protein knockout mediated by anti-GFP nanobody
暂无分享,去创建一个
[1] Akihiro Urasaki,et al. zTrap: zebrafish gene trap and enhancer trap database , 2010, BMC Developmental Biology.
[2] Tatsuo Fukagawa,et al. An auxin-based degron system for the rapid depletion of proteins in nonplant cells , 2009, Nature Methods.
[3] K. Kawakami,et al. The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish. , 2009, Methods.
[4] S. Schornack,et al. Protein mislocalization in plant cells using a GFP-binding chromobody. , 2009, The Plant journal : for cell and molecular biology.
[5] Wei Dong,et al. Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering , 2009, Proceedings of the National Academy of Sciences.
[6] Michael Knop,et al. Efficient protein depletion by genetically controlled deprotection of a dormant N-degron , 2009, Molecular systems biology.
[7] M. Affolter,et al. Tip-Cell Migration Controls Stalk-Cell Intercalation during Drosophila Tracheal Tube Elongation , 2008, Current Biology.
[8] U. K. Laemmli,et al. The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes. , 2008, Molecular cell.
[9] R. Schuh,et al. TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organisms. , 2008, BioTechniques.
[10] C. Lehner,et al. Cell-Type-Specific TEV Protease Cleavage Reveals Cohesin Functions in Drosophila Neurons , 2008, Developmental cell.
[11] M. Cristina Cardoso,et al. A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins*S , 2008, Molecular & Cellular Proteomics.
[12] Daryl M. Gohl,et al. Enhancer Blocking and Transvection at the Drosophila apterous Locus , 2008, Genetics.
[13] Roger A Hoskins,et al. The Carnegie Protein Trap Library: A Versatile Tool for Drosophila Developmental Studies , 2007, Genetics.
[14] R. Hoskins,et al. Exploring Strategies for Protein Trapping in Drosophila , 2007, Genetics.
[15] R. Maeda,et al. An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases , 2007, Proceedings of the National Academy of Sciences.
[16] Heinrich Leonhardt,et al. Targeting and tracing antigens in live cells with fluorescent nanobodies , 2006, Nature Methods.
[17] L. Banaszynski,et al. A Rapid, Reversible, and Tunable Method to Regulate Protein Function in Living Cells Using Synthetic Small Molecules , 2006, Cell.
[18] E. Nigg,et al. HURP Is a Ran-Importin β-Regulated Protein that Stabilizes Kinetochore Microtubules in the Vicinity of Chromosomes , 2006, Current Biology.
[19] Nathan C Shaner,et al. A guide to choosing fluorescent proteins , 2005, Nature Methods.
[20] L. Wyns,et al. Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies. , 2005, Journal of molecular biology.
[21] Tobias Meyer,et al. An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways , 2005, Nature Methods.
[22] Nancy A. Jenkins,et al. Simple and highly efficient BAC recombineering using galK selection , 2005, Nucleic acids research.
[23] A. Ciechanover,et al. N-terminal ubiquitination: more protein substrates join in. , 2004, Trends in cell biology.
[24] R. Karess,et al. Reassessing the role and dynamics of nonmuscle myosin II during furrow formation in early Drosophila embryos. , 2003, Molecular biology of the cell.
[25] N. Zheng,et al. Exploring the functional complexity of cellular proteins by protein knockout , 2003, Proceedings of the National Academy of Sciences of the United States of America.
[26] X. Morin,et al. A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila , 2001, Proceedings of the National Academy of Sciences of the United States of America.
[27] R. Deshaies,et al. Protacs: Chimeric molecules that target proteins to the Skp1–Cullin–F box complex for ubiquitination and degradation , 2001, Proceedings of the National Academy of Sciences of the United States of America.
[28] S. Tsukita,et al. Real-time imaging of cell-cell adherens junctions reveals that Drosophila mesoderm invagination begins with two phases of apical constriction of cells. , 2001, Journal of cell science.
[29] P. Howley,et al. Harnessing the ubiquitination machinery to target the degradation of specific cellular proteins. , 2000, Molecular cell.
[30] Wayne L. Rickoll,et al. Multiple Forces Contribute to Cell Sheet Morphogenesis for Dorsal Closure in Drosophila , 2000, The Journal of cell biology.
[31] Aaron Ciechanover,et al. The ubiquitin–proteasome pathway: on protein death and cell life , 1998, The EMBO journal.
[32] G. Struhl,et al. Regulation of the Hedgehog and Wingless signalling pathways by the F-box/WD40-repeat protein Slimb , 1998, Nature.
[33] A. Varshavsky,et al. Heat-inducible degron: a method for constructing temperature-sensitive mutants. , 1994, Science.
[34] N. Perrimon,et al. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. , 1993, Development.
[35] T. Tabata,et al. The Drosophila hedgehog gene is expressed specifically in posterior compartment cells and is a target of engrailed regulation. , 1992, Genes & development.
[36] K. Edwards,et al. The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila. , 1991, Cell.
[37] A. Wodarz. Extraction and immunoblotting of proteins from embryos. , 2008, Methods in molecular biology.
[38] Lynn Cooley,et al. Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster , 2004, Nucleic Acids Res..
[39] D. Kiehart,et al. Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function. , 1993, Genes & development.