DNA typing of cellular material on perforating bullets

DNA typing of cellular debris from perforating bullets was investigated following shooting experiments. A total of 14 perforating gunshots were fired into 9 calves. PCR typing of tissue fragments was done using bovinespecific primers flanking a 247 by segment within the bovine lactoglobulin gene. Positive amplification results were obtained for all 9 hollow point (HP) and all 5 full metal jacket (FMJ) bullets. In contrast to HP bullets the smooth surfaces of the FMJ bullets did not have visible biological material, which resulted in weaker bands in the DNA analysis compared to HP bullets. Tissue seemed to accumulate at the base of the projectiles. Due to the lack of a suitable marker in bovines, only a species identification was carried out on the DNA from tissue on the bullets. The small amount of DNA extract (up to 5%) required for specification is promising for the successful application of a set of short tandem repeat (STR) systems for individualization in humans. By individualizing tissue on perforating bullets, the bullet and the victim it passed through can be linked. This can assist the investigation of gunshot deaths, especially when several persons are involved in a gun fight.