Models for low resolution slit scan measurements based on high resolution laser scanning image analysis: DNA and nuclear dimensions

Abstract A laser scanning microscope has been developed and employed to make high precision measurements of cells from clinical cervical samples. These specimens have been stained with the DNA specific fluorochrome mithramycin and the fluorescent images analyzed to give information on nuclear size, DNA content and nuclear shape. This information has been used to develop low resolution parameters as measured with flow cytometers, thus permitting some comparisons between high resolution and low resolution features as measured from the same cells. The results indicate that both low resolution flow slit scan and high resolution pattern recognition techniques yield the same average cellular information. However, low resolution methods are insensitive to shape and the influence of shape on the broadening of low resolution features is now under investigation.