BIO AND IMMUNO‐ACTIVITY OF FSH IN SERUM AFTER INTRAMUSCULAR INJECTION OF HIGHLY PURIFIED URINARY HUMAN FSH IN NORMAL MEN

The pharmacokinetics of a human FSH preparation widely used in clinical practice was evaluated in healthy male volunteers whose endogenous FSH was suppressed by 19‐nortestosterone injections. 9‐7 h following intramuscular injection of 150 IU FSH the mean immunoreactive peak concentration over baseline was 3–8 IU/1. Injection of 450 IU FSH led to a mean immunoreactive peak level of 10‐4 IU/1 at 9‐8 h. Mean half‐life was 24‐6 h (150 IU FSH) and 36‐2 h (450 IU FSH). Serum bioFSH concentrations were suppressed below the detection limit after injection of the 19‐nortestosterone and rose to preinjection levels after administration of FSH. Mean bioactive peak level for the 450 IU FSH was 7‐3 IU/1 FSH at 7‐4 h. The half‐life of bioFSH was calculated to be 13‐4 h and is considerably shorter than the half‐life of immunoFSH. Serum inhibin levels were suppressed by 19‐nortestosterone and did not change significantly after injection of the FSH preparation, suggesting that the FSH dose was insufficient to elicit this biological response. It is concluded that the currently used dose regimen for the treatment of male hypogonadism is inadequate for maintenace of serum bioFSH levels in the normal range.

[1]  E. Nieschlag,et al.  Follicle-stimulating hormone stimulates inhibin in the serum of male monkeys (Macaca mulatta). , 1990, Acta endocrinologica.

[2]  E. Nieschlag,et al.  Diagnostic value of bioactive FSH in male infertility. , 1989, Acta endocrinologica.

[3]  E. Nieschlag,et al.  Combination of 19-nortestosterone-hexyloxyphenylpropionate (Anadur) and depot-medroxyprogesterone-acetate (Clinovir) for male contraception. , 1989 .

[4]  H. Burger,et al.  Serum inhibin levels in normal men and men with testicular disorders. , 1989, The Journal of endocrinology.

[5]  V. P. Johnson,et al.  Gonadotropin-induced fertility in a man with congenital hypogonadism after prolonged prior testosterone therapy. , 1988, South Dakota journal of medicine.

[6]  S. Banks,et al.  Two-year comparison of testicular responses to pulsatile gonadotropin-releasing hormone and exogenous gonadotropins from the inception of therapy in men with isolated hypogonadotropic hypogonadism. , 1988, The Journal of clinical endocrinology and metabolism.

[7]  H. Burger,et al.  Relative roles of follicle-stimulating hormone and luteinizing hormone in the control of inhibin secretion in normal men. , 1988, The Journal of clinical investigation.

[8]  David Handelsman,et al.  Comparison of inhibin immunological and in vitro biological activities in human serum. , 1988, The Journal of clinical endocrinology and metabolism.

[9]  C. Wang.  Bioassays of follicle stimulating hormone. , 1988, Endocrine reviews.

[10]  E. Diczfalusy,et al.  Clinical-pharmacological studies on human menopausal gonadotrophin. , 1988, Human reproduction.

[11]  E. Nieschlag,et al.  Treatment of severe oligospermia with human chorionic gonadotropin/human menopausal gonadotropin: a placebo-controlled, double blind trial. , 1987, The Journal of clinical endocrinology and metabolism.

[12]  H. Burger,et al.  Circulating immunoreactive inhibin levels during the normal human menstrual cycle. , 1987, The Journal of clinical endocrinology and metabolism.

[13]  A. Hsueh,et al.  Serum bioactive follicle-stimulating hormone in men with idiopathic azoospermia and oligospermia. , 1987, The Journal of clinical endocrinology and metabolism.

[14]  V. Padmanabhan,et al.  An improved in vitro bioassay for follicle-stimulating hormone (FSH): suitable for measurement of FSH in unextracted human serum. , 1987, Endocrinology.

[15]  W. Collins,et al.  Studies on the measurement and pharmacodynamics of human follicle-stimulating hormone. , 1987, Fertility and sterility.

[16]  E. Diczfalusy,et al.  Molecular composition of luteinizing hormone and follicle-stimulating hormone in commercial gonadotropin preparations. , 1986, Fertility and sterility.

[17]  E. Nieschlag,et al.  Clinical trial of 19-nortestosterone-hexoxyphenylpropionate (Anadur) for male fertility regulation. , 1985, Fertility and Sterility.

[18]  E. Nieschlag,et al.  Pharmacokinetics of 19-nortestosterone esters in normal men. , 1985, Journal of steroid biochemistry.

[19]  E. Nieschlag,et al.  REVERSIBLE AZOOSPERMIA INDUCED BY THE ANABOLIC STEROID 19-NORTESTOSTERONE , 1984, The Lancet.

[20]  C. Coutifaris,et al.  Biosynthesis and secretion of follicle-stimulating hormone. , 1983, Endocrine reviews.

[21]  P. Franchimont,et al.  Regulation of inhibin secretion by Sertoli cell-enriched cultures. , 1983, Acta endocrinologica.

[22]  D. Kretser,et al.  Inhibin production by sertoli cell cultures , 1982, Molecular and Cellular Endocrinology.

[23]  W. Schill,et al.  Combined hMG/hCG treatment in subfertile men with idiopathic normogonadotrophic oligozoospermia. , 1982, International journal of andrology.

[24]  F. Le Gac,et al.  Inhibin production by Sertoli cell cultures. , 1982, Molecular and cellular endocrinology.

[25]  E. Diczfalusy,et al.  Studies on the biological and immunological properties of human follitropin: profiles of two international reference preparations and of an aqueous extract of pituitary glands after electrofocusing. , 1981, Acta endocrinologica.

[26]  E. Diczfalusy,et al.  A sensitive and specific in vitro bioassay method for the measurement of follicle-stimulating hormone activity. , 1979, Acta endocrinologica.

[27]  J. Marshall,et al.  Infusions of hFSH and hLH in normal men. I. Kinetics of human follicle stimulating hormone. , 1976, Acta endocrinologica.

[28]  H. K. Amin,et al.  Human pituitary follicle-stimulating hormone: distribution, plasma clearance and urinary excretion as determined by radioimmunoassay. , 1970, Journal of Endocrinology.

[29]  A. Littell,et al.  Disappearance rates of endogenous follicle-stimulating hormone in serum following surgical hypophysectomy in man. , 1970, The Journal of clinical endocrinology and metabolism.

[30]  T. Fukushima.  [Bioassay of the follicle-stimulating hormone]. , 1969, Nihon Naibunpi Gakkai zasshi.