Functional correlation between cell adhesive properties and some cell surface proteins

The adhesive properties of Chinese hamster V79 cells were analyzed and characterized by various cell dissociation treatments. The comparisons of aggregability among cells dissociated with EDTA, trypsin + Ca2+, and trypsin + EDTA, revealed that these cells have two adhesion mechanisms, a Ca2+-independent and a Ca2+-dependent one. The former did not depend on temperature, whereas the latter occurred only at physiological temperatures. Both mechanisms were trypsin sensitive, but the Ca2+- dependent one was protected by Ca2+ against trypsinization. In morphological studies, the Ca2+-independent adhesion appeared to be a simple agglutination or flocculation of cells, whereas the Ca2+- dependent adhesion seemed to be more physiological, being accompanied by cell deformation resulting in the increase of contact area between adjacent cells. Lactoperoxidase-catalyzed iodination of cell surface proteins revealed that several proteins are more intensely labeled in cells with Ca2+-independent adhesiveness than in cells without that property. It was also found that a cell surface protein with a molecular weight of approximately 150,000 is present only in cells with Ca2+-dependent adhesiveness. The iodination and trypsinization of this protein were protected by Ca2+, suggesting its reactivity to Ca2+. Possible mechanisms for each adhesion property are discussed, taking into account the correlation of these proteins with cell adhesiveness.

[1]  S. V. Perry,et al.  [94] Myosin adenosinetriphosphatase: ATP H2O→ADP+H3PO4 , 1955 .

[2]  G. Yerganian,et al.  Observations on the chromosomes of Chinese hamster cells in tissue culture. , 1958, Journal of the National Cancer Institute.

[3]  T. Puck,et al.  GENETICS OF SOMATIC MAMMALIAN CELLS III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS , 1958 .

[4]  G. Yerganian,et al.  Further observations on the chromosomes of Chinese hamster cells in tissue culture. , 1959, Journal of the National Cancer Institute.

[5]  M. Greaves,et al.  The inhibition of cell aggregation by a pure serum protein. , 1965, Journal of embryology and experimental morphology.

[6]  M. Collins.  Electrokinetic properties of dissociated chick embryo cells. Calcium ion binding by neural retinal cells. , 1966, The Journal of experimental zoology.

[7]  A. Moscona,et al.  Aggregation of embryonic cells in a serum-free medium and its inhibition at suboptimal temperatures. , 1966, Experimental cell research.

[8]  P. Armstrong,et al.  On the role of metal cations in ceullular adhesion: cation specificity. , 1968, The Journal of experimental zoology.

[9]  D. Phillips,et al.  The arrangement of proteins in the human erythrocyte membrane. , 1970, Biochemical and biophysical research communications.

[10]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[11]  J. Campbell,et al.  The aggregation of trypsinized BHK21 cells. , 1971, Journal of cell science.

[12]  A. Hubbard,et al.  THE ENZYMATIC IODINATION OF THE RED CELL MEMBRANE , 1972, The Journal of cell biology.

[13]  D. Phillips.  Effect of trypsin on the exposed polypeptides and glycoproteins in the human platelet membrane. , 1972, Biochemistry.

[14]  M. G. Vicker,et al.  The effect of neuraminidase on the aggregation of BHK21 cells and BHK21 cells transformed by polyoma virus. , 1972, Journal of cell science.

[15]  M. Takeichi,et al.  Roles of magnesium and calcium ions in cell-to-substrate adhesion. , 1972, Experimental cell research.

[16]  P. Stambrook,et al.  INDUCED CHANGES IN THE RATES OF URIDINE-3H UPTAKE AND INCORPORATION DURING THE G1 AND S PERIODS OF SYNCHRONIZED CHINESE HAMSTER CELLS , 1972, The Journal of cell biology.

[17]  J. E. Hornby.  Measurements of cell adhesion. II. Quantitative study of the effect of divalent ions on cell adhesion. , 1973, Journal of embryology and experimental morphology.

[18]  M Edidin,et al.  Rotational and translational diffusion in membranes. , 1974, Annual review of biophysics and bioengineering.

[19]  J. Deman.  INTERCELLULAR ADHESION Effects of Neuraminidase, Calcium, and Trypsin on the Aggregation of Suspended HeLa Cells , 1974 .

[20]  M. Mareel,et al.  A STUDY ON THE MECHANISM OF INTERCELLULAR ADHESION , 1974, The Journal of cell biology.

[21]  M. G. Vicker,et al.  Trypsinized BHK21 cells aggregate in the presence of metabolic inhibitors and in the absence of divalent cations. , 1975, Journal of cell science.

[22]  R. Juliano,et al.  An evaluation of techniques for labelling the surface proteins of cultured mammalian cells. , 1975, Biochimica et biophysica acta.

[23]  G. Edelman,et al.  Mechanisms of adhesion among cells from neural tissues of the chick embryo. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[24]  K. Vosbeck,et al.  Assay of intercellular adhesiveness using cell-coated Sephadex beads as collecting particles. , 1976, Journal of cell science.

[25]  C. Lloyd,et al.  Mechanisms of cell adhesion: early-forming junctions between aggregating fibroblasts. , 1976, Journal of cell science.

[26]  G. Nicolson.  Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over surface components. , 1976, Biochimica et biophysica acta.

[27]  A. Hakura,et al.  Different cation requirements for aggregation of BHK cells and their transformed derivatives. , 1976, Journal of cell science.

[28]  M. Takeichi,et al.  Two Mechanisms in Cell Adhesion Revealed by Effects of Divalent Cations , 1976 .

[29]  E. McGuire,et al.  Intercellular adhesive selectivity. II. Properties of embryonic chick liver cell-cell adhesion , 1976, The Journal of cell biology.

[30]  J. Meldolesi,et al.  Extracellular calcium and the organization of tight junctions in pancreatic acinar cells. , 1976, Experimental cell research.

[31]  M. Takeichi,et al.  Adhesion of phospholipid vesicles to Chinese hamster fibroblasts: Role of cell surface proteins , 1977, The Journal of cell biology.