Comparative Spectrophotometric and Chromatographic Assessment of Antioxidant Capacity in Different Marine Algae

ABSTRACT The objective of the current study was to analyze the vital phenolic compounds available in the marine macroalgae (mostly edible) collected from the southwest coast of Turkey, to compute their total antioxidant capacity (TAC) with the cupric ion reducing antioxidant capacity (CUPRAC) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) assays and to correspond their antioxidant power with high performance liquid chromatography (HPLC) findings. The predominant constitutive phloroglucinol unit of the phlorotannins was the main phenolic component in brown algae species. Among the tested species, those with the greatest phloroglucinol content found by HPLC were Cladostephus spongiosum f.verticillatum (31.62 ± 0.66 μmolg−1), Cystoseira barbata (13.34 ± 0.60 μmolg−1), and Dictyota dichotoma (11.23 ± 0.19 μmolg−1), respectively.

[1]  Hulya Civelek Yoruklu,et al.  Optimization of liquid fertilizer production from waste seaweed: A design of experiment based statistical approach. , 2021, Chemosphere.

[2]  S. Passamonti,et al.  Four selected commercial seaweeds: biologically active compounds, antioxidant and cytotoxic properties , 2021, International journal of food sciences and nutrition.

[3]  S. Yalcin,et al.  HPLC Detection and Antioxidant Capacity Determination of Brown, Red and Green Algal Pigments in Seaweed Extracts. , 2020, Journal of chromatographic science.

[4]  S. Yalcin,et al.  Determination of Total Antioxidant Capacities of Algal Pigments in Seaweed by the Combination of High-Performance Liquid Chromatography (HPLC) with A Cupric Reducing Antioxidant Capacity (CUPRAC) Assay , 2020, Analytical Letters.

[5]  S. Khan.  Environmental sustainability: a clean energy aspect versus poverty , 2020, Environmental Science and Pollution Research.

[6]  J. Simal-Gándara,et al.  Main bioactive phenolic compounds in marine algae and their mechanisms of action supporting potential health benefits. , 2020, Food chemistry.

[7]  A. Critchley,et al.  The COVID 19 novel coronavirus pandemic 2020: seaweeds to the rescue? Why does substantial, supporting research about the antiviral properties of seaweed polysaccharides seem to go unrecognized by the pharmaceutical community in these desperate times? , 2020, Journal of Applied Phycology.

[8]  A. Critchley,et al.  The COVID 19 novel coronavirus pandemic 2020: seaweeds to the rescue? Why does substantial, supporting research about the antiviral properties of seaweed polysaccharides seem to go unrecognized by the pharmaceutical community in these desperate times? , 2020, Journal of Applied Phycology.

[9]  M. Antunes-Ricardo,et al.  Ultrasound-assisted extraction of phlorotannins and polysaccharides from Silvetia compressa (Phaeophyceae) , 2020, Journal of Applied Phycology.

[10]  Pınar Terzioğlu,et al.  In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical fingerprints of five Moroccan seaweeds , 2020 .

[11]  İ. Ak,et al.  Antioxidant Activities of Eucheuma sp. (Rhodophyceae) and Laminaria sp. (Phaeophyceae) , 2019 .

[12]  J. Camp,et al.  Antioxidant activity, total phenolics and flavonoids contents: Should we ban in vitro screening methods? , 2018, Food chemistry.

[13]  O. Radjasa,et al.  Total phenolic content and biological activities of enzymatic extracts from Sargassum muticum (Yendo) Fensholt , 2017, Journal of Applied Phycology.

[14]  Xiaoyong Liu,et al.  Extraction and Identification of Phlorotannins from the Brown Alga, Sargassum fusiforme (Harvey) Setchell , 2017, Marine drugs.

[15]  B. Prieto,et al.  Extraction of polyphenols in Himanthalia elongata and determination by high performance liquid chromatography with diode array detector prior to its potential use against oxidative stress. , 2016, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[16]  B. Foley,et al.  Identification and characterization of phenolic antioxidant compounds from brown Irish seaweed Himanthalia elongata using LC-DAD–ESI-MS/MS , 2016 .

[17]  E. Çapanoğlu,et al.  Antioxidant Activity/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays. , 2016, Journal of agricultural and food chemistry.

[18]  L. Pereira.  Edible Seaweeds of the World , 2016 .

[19]  D. Pádua,et al.  Bioactive compounds from brown seaweeds: Phloroglucinol, fucoxanthin and fucoidan as promising therapeutic agents against breast cancer , 2015 .

[20]  Per Møller,et al.  Carotenoids, Phenolic Compounds and Tocopherols Contribute to the Antioxidative Properties of Some Microalgae Species Grown on Industrial Wastewater , 2015, Marine drugs.

[21]  F. Shahidi,et al.  Measurement of antioxidant activity , 2015 .

[22]  P. Varalakshmi,et al.  Phlorotannins from Brown Algae: inhibition of advanced glycation end products formation in high glucose induced Caenorhabditis elegans. , 2015, Indian journal of experimental biology.

[23]  J. Mlček,et al.  Phenolic Content and Antioxidant Capacity in Algal Food Products , 2015, Molecules.

[24]  M. Lila,et al.  Phlorotannins from Alaskan Seaweed Inhibit Carbolytic Enzyme Activity , 2014, Marine drugs.

[25]  F. Karadeniz,et al.  Anti-HIV-1 activity of phlorotannin derivative 8,4‴-dieckol from Korean brown alga Ecklonia cava , 2014, Bioscience, biotechnology, and biochemistry.

[26]  S. Nabavi,et al.  Antioxidant Activity, Total Phenolics and Flavonoid Contents of some Edible Green Seaweeds from Northern Coasts of the Persian Gulf , 2014, Iranian journal of pharmaceutical research : IJPR.

[27]  N. Brunton,et al.  Enrichment of polyphenol contents and antioxidant activities of Irish brown macroalgae using food-friendly techniques based on polarity and molecular size. , 2013, Food chemistry.

[28]  C. Jacobsen,et al.  Phenolic compounds and antioxidant activities of selected species of seaweeds from Danish coast. , 2013, Food chemistry.

[29]  N. Hamid,et al.  Fucoxanthin content and antioxidant properties of Undaria pinnatifida. , 2013, Food chemistry.

[30]  R. Jónsdóttir,et al.  Antioxidant capacities of phlorotannins extracted from the brown algae Fucus vesiculosus. , 2012, Journal of agricultural and food chemistry.

[31]  K. Miyashita,et al.  Comparative antioxidant activity of edible Japanese brown seaweeds. , 2011, Journal of food science.

[32]  D. Hauchard,et al.  Phenolic compounds in the brown seaweed Ascophyllum nodosum: distribution and radical-scavenging activities. , 2010, Phytochemical analysis : PCA.

[33]  K. Başkan,et al.  Combined HPLC-CUPRAC (cupric ion reducing antioxidant capacity) assay of parsley, celery leaves, and nettle. , 2008, Talanta.

[34]  K. Pihlaja,et al.  High-performance liquid chromatographic analysis of phlorotannins from the brown alga Fucus vesiculosus. , 2007, Phytochemical analysis : PCA.

[35]  R. Pedreschi,et al.  Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruíz & Pavón) tubers , 2007 .

[36]  R. Prior,et al.  Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. , 2005, Journal of agricultural and food chemistry.

[37]  R. Apak,et al.  Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. , 2004, Journal of agricultural and food chemistry.

[38]  A. Coleman,et al.  Phylogenetic relationships within the genus Sargassum (Fucales, Phaeophyceae), inferred from ITS‐2 nrDNA, with an emphasis on the taxonomic subdivision of the genus , 2003 .

[39]  D. Petillo,et al.  Distribution of catechins in Japanese seaweeds , 2000 .

[40]  C. Rice-Evans,et al.  Antioxidant activity applying an improved ABTS radical cation decolorization assay. , 1999, Free radical biology & medicine.

[41]  R. Lamuela-Raventós,et al.  Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent , 1999 .

[42]  C. Berset,et al.  Use of a Free Radical Method to Evaluate Antioxidant Activity , 1995 .