Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy

A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.

[1]  B. Krauskopf,et al.  Proc of SPIE , 2003 .

[2]  Demetri Psaltis,et al.  Holographic Data Storage , 1998, Computer.

[3]  Raymond K. Kostuk,et al.  Simulations and experiments of aperiodic and multiplexed gratings in volume holographic imaging systems , 2010, Optics express.

[4]  Bane Vasic,et al.  Cascaded holographic polymer reflection grating filters for optical-code-division multiple-access applications. , 2005, Applied optics.

[5]  Se Baek Oh,et al.  Wavelength-coded multifocal microscopy. , 2010, Optics letters.

[6]  Brian H. Marcus,et al.  Holographic data storage technology , 2000, IBM J. Res. Dev..

[7]  R. Kamm,et al.  Cell migration into scaffolds under co-culture conditions in a microfluidic platform. , 2009, Lab on a chip.

[8]  Demetri Psaltis,et al.  Volume holographic spectral imaging , 2005, SPIE BiOS.

[9]  Demetri Psaltis,et al.  Real-time spectral imaging in three spatial dimensions. , 2002, Optics letters.

[10]  George Barbastathis,et al.  Silicon oxide nanoparticles doped PQ-PMMA for volume holographic imaging filters. , 2010, Optics Letters.

[11]  A. Rouse,et al.  Monte Carlo characterization of parallelized fluorescence confocal systems imaging in turbid media. , 2009, Journal of biomedical optics.

[12]  B. Bouma,et al.  Handbook of Optical Coherence Tomography , 2001 .

[13]  Yuan Luo,et al.  Optimization of multiplexed holographic gratings in PQ-PMMA for spectral-spatial imaging filters. , 2008, Optics letters.

[14]  N Iftimia,et al.  Rapid wavelength-swept spectrally encoded confocal microscopy. , 2005, Optics express.

[15]  Atsushi Sato,et al.  Holographic edge-illuminated polymer Bragg gratings for dense wavelength division optical filters at 1550 nm. , 2003, Applied optics.