Silica Particles with Human Protein Corona Shows Sensitization Potential in the Human Cell Line Activation Test

potential in acquired immunity is The human cell line activation test (h-CLAT) is an in vitro skin sensitization test in Organization Co-operation and Development In the h-CLAT, the sensitization potential in the development of acquired immunity of a chemical substance is evaluated by exposing human monocytic leukemia THP-1 cells to the test material and examining the changes in expression of the cell-surface markers CD86 and CD54, which is increased when the cells are activated. 9) Here, we attempted to evaluate sensitization of silica particles and to explore the relationship among nanoparticles’ size, their status, and sensitizing potential by using the h-CLAT. Nanoparticles are concerned to show adverse biological effects despite their unique functions. Their physico chemical properties and status are widely diverse; this makes safety analysis of nanoparticles complicated. Some reports showed that nanoparticles could disturb acquired immunity, while it is still unclear what is the inducer of that effects. Here, we tried to explore the relationship among nanoparticles’ physicochemical properties and sensitizing potential by using the human cell line activation test based in vitro method; that uses in expression of CD86 and CD54 as an index of cellular activation. As a model of nanoparticles, we examined sensitization poten tial of silica particles with or without a human protein corona. Of the cells treated with silica particles (diam -eter: 50 or 300 nm) only, none of them showed activation. On the other hand, silica particles with human pro tein corona showed activation. Moreover, protein corona that forms around 50 nm silica nanoparticles have a higher sensitization potential than that of protein corona that forms around 300 nm silica particles. Our findings indicated that silica particles with human protein corona showed sensitization potential, and that sensitization potential could depend on the amount or kind of proteins within the corona.

[1]  Yugang Wang,et al.  Exploration of Antigen Induced CaCO3 Nanoparticles for Therapeutic Vaccine. , 2018, Small.

[2]  M. Nakayama,et al.  Macrophage Recognition of Crystals and Nanoparticles , 2018, Front. Immunol..

[3]  R. Grandori,et al.  Formation of the Protein Corona: The Interface between Nanoparticles and the Immune System. , 2017, Seminars in immunology.

[4]  K. Ishii,et al.  Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific , 2017, Front. Immunol..

[5]  M. Sayan,et al.  The NLRP3 inflammasome in pathogenic particle and fibre-associated lung inflammation and diseases , 2015, Particle and Fibre Toxicology.

[6]  S. Khotimchenko,et al.  Nanomaterials in consumer's goods: the problems of risk assessment , 2015 .

[7]  C. Riggio,et al.  The effect of surface charge of functionalized Fe3O4 nanoparticles on protein adsorption and cell uptake. , 2014, Biomaterials.

[8]  Stefan Tenzer,et al.  Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology. , 2013, Nature nanotechnology.

[9]  Changyou Gao,et al.  Molecular interactions of different size AuNP-COOH nanoparticles with human fibrinogen. , 2013, Nanoscale.

[10]  Shyh-Dar Li,et al.  Immune responses of therapeutic lipid nanoparticles , 2013 .

[11]  Rodney F. Minchin,et al.  Molecular interaction of poly(acrylic acid) gold nanoparticles with human fibrinogen. , 2012, ACS nano.

[12]  Stefan Tenzer,et al.  Nanoparticle size is a critical physicochemical determinant of the human blood plasma corona: a comprehensive quantitative proteomic analysis. , 2011, ACS nano.

[13]  D. Simberg,et al.  Interactions of nanoparticles with plasma proteins: implication on clearance and toxicity of drug delivery systems , 2011, Expert opinion on drug delivery.

[14]  Naomi J Halas,et al.  Gold nanoparticles can induce the formation of protein-based aggregates at physiological pH. , 2009, Nano letters.

[15]  Richard A. Flavell,et al.  The Nalp3 inflammasome is essential for the development of silicosis , 2008, Proceedings of the National Academy of Sciences.

[16]  Sara Linse,et al.  Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles , 2007, Proceedings of the National Academy of Sciences.

[17]  H. Sakaguchi,et al.  Development of an in vitro skin sensitization test using human cell lines: the human Cell Line Activation Test (h-CLAT). I. Optimization of the h-CLAT protocol. , 2006, Toxicology in vitro : an international journal published in association with BIBRA.

[18]  David Farrar,et al.  Interpretation of protein adsorption: surface-induced conformational changes. , 2005, Journal of the American Chemical Society.

[19]  OECD GUIDELINES FOR THE TESTING OF CHEMICALS , 2014 .