Characterization and biotechnological potential of petroleum-degrading bacteria isolated from oil-contaminated soils.

A collection of 38 bacteria was obtained by enrichment cultivation from oil-contaminated soils of an oil field in Daqing, China. Twenty-two strains could utilize diesel oil as the sole source of carbon and energy, and 11 strains could degrade the total petroleum hydrocarbons (TPHs) of diesel oil by more than 70% in 7d. Phylogenetically, 19 of the bacteria related to Bacillus species. About 87.5% TPHs of crude oil were degraded by a consortium of seven strains. Denaturing gradient gel electrophoresis analysis suggested that five of the strains persisted throughout the degradation process. The collection of isolated bacteria might be a useful resource for bioremediation of oil-contaminated soils and biotreatment of oil wastewater.

[1]  J. Tiedje,et al.  Multi-locus real-time PCR for quantitation of bacteria in the environment reveals Exiguobacterium to be prevalent in permafrost. , 2007, FEMS microbiology ecology.

[2]  D. de Mendoza,et al.  The Bacillus subtilis desaturase: a model to understand phospholipid modification and temperature sensing , 2005, Archives of Microbiology.

[3]  P. Xu,et al.  Deep desulfurization of hydrodesulfurization-treated diesel oil by a facultative thermophilic bacterium Mycobacterium sp. X7B. , 2003, FEMS microbiology letters.

[4]  J. Cooney,et al.  Response of spores and vegetative cells of Bacillus spp. in a hydrocarbon-water system. , 1971, The Journal of applied bacteriology.

[5]  Ramaraj Boopathy,et al.  Factors limiting bioremediation technologies , 2000, Bioresource Technology.

[6]  R. Kuhad,et al.  In Situ Bioremediation Potential of an Oily Sludge-Degrading Bacterial Consortium , 2001, Current Microbiology.

[7]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[8]  L. F. Bautista,et al.  Isolation and genetic identification of PAH degrading bacteria from a microbial consortium , 2009, Biodegradation.

[9]  D. Lane.  16S/23S rRNA sequencing , 1991 .

[10]  A. Uitterlinden,et al.  Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA , 1993, Applied and environmental microbiology.

[11]  V. Pruthi,et al.  Oily sludge degradation by bacteria from Ankleshwar, India , 2006 .

[12]  P. Grimont,et al.  Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms. , 2004, Research in microbiology.

[13]  Owen P. Ward,et al.  Recent Advances in Petroleum Microbiology , 2003, Microbiology and Molecular Biology Reviews.

[14]  J. Retief,et al.  Phylogenetic analysis using PHYLIP. , 2000, Methods in molecular biology.

[15]  B. Okeke,et al.  Microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil. , 2008, Bioresource technology.

[16]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[17]  E. Stackebrandt,et al.  Nucleic acid techniques in bacterial systematics , 1991 .