Molecular Diagnosis of Bordetella pertussis Infection by Evaluation of Formalin-Fixed Tissue Specimens

ABSTRACT Formalin-fixed lung or trachea tissue specimens from four infants and one adolescent who died of respiratory illness were tested for Bordetella pertussis by conventional and real-time PCR assays. B. pertussis was confirmed in all cases. PCR can be an invaluable retrospective diagnostic tool for evaluating archival tissues from patients with suspected fatal pertussis.

[1]  J. Cherry,et al.  Bordetella pertussis infections and sudden unexpected deaths in children , 1996, European Journal of Pediatrics.

[2]  J. Cherry,et al.  A controlled study of the relationship between Bordetella pertussis infections and sudden unexpected deaths among German infants. , 2004, Pediatrics.

[3]  H. Goossens,et al.  Evaluation of Real-Time PCR for Detection of and Discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for Clinical Diagnosis , 2003, Journal of Clinical Microbiology.

[4]  C. Vitek,et al.  Increase in deaths from pertussis among young infants in the United States in the 1990s , 2003, The Pediatric infectious disease journal.

[5]  M. Loeffelholz,et al.  Real-Time PCR Assay Targeting IS481of Bordetella pertussis and Molecular Basis for Detecting Bordetella holmesii , 2001, Journal of Clinical Microbiology.

[6]  P. A. Hartley,et al.  Bordetella holmesii-like organisms associated with septicemia, endocarditis, and respiratory failure. , 1998, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[7]  H. Lagercrantz,et al.  Sudden infant death and prevalence of whooping cough in the Swedish and Norwegian communities , 1997, European Journal of Pediatrics.

[8]  C. W. Moss,et al.  Bordetella holmesii sp. nov., a new gram-negative species associated with septicemia , 1995, Journal of clinical microbiology.

[9]  F. Mooi,et al.  Polymerase chain reaction assay for pertussis: simultaneous detection and discrimination of Bordetella pertussis and Bordetella parapertussis , 1993, Journal of clinical microbiology.

[10]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[11]  A. Nicoll,et al.  Whooping cough and unrecognised postperinatal mortality. , 1988, Archives of disease in childhood.