Possible compensation of structural and viscotropic properties in hepatic microsomes and erythrocyte membranes of rats with essential fatty acid deficiency.

The effect of essential fatty acid deficiency on the structural and dynamic properties of the lipid matrix of rat liver microsomes and erythrocyte membranes was studied. The rate and range of the rotational mobility of 1,6-diphenyl-1,3,5-hexatriene and 2-, 7-, and 12-(9-anthroyloxy)stearate probes in the native membranes and in lipid vesicles prepared with the total lipid extracts of these membranes were evaluated by using differential polarized phase fluorometry. For the anthroyloxystearate probes, two modes of rotation (in and out of the plane of the aromatic anthracene ring) were partially resolved by measuring at different excitation wavelengths. The fat-free diet produces important changes in the fatty acid composition of the different glycerophospholipid classes without affecting the total double-bond number, the relative contents of cholesterol, phospholipid, and protein, and the glycerophospholipid class distribution. The principal changes, more pronounced in liver microsomes than in erythrocytes, are: an increase in nonessential monoene and triene (18:1n-9 and 20:3n-9) and a decrease in essential diene (18:2n-6) and tetraene (20:4n-6). These changes modify the double-bond distribution as a function of the distance from the interphase toward the bilayer interior, with a significant deficit (15% in erythrocytes and 30% in liver microsomes) in the double-bond density in the intermediate region of the membrane leaflet, corresponding to the carbon number 11-12 of an extended saturated acyl chain, and where the 12-anthroyloxystearate probe is located. In spite of the changes in fatty acid composition and double-bond distribution, with the only exception of a slight increase (about 15%) in the "out of the plane" rotation rate of the 7-(9-anthroyloxy)stearate probe in the erythrocyte lipid vesicles, no other significant change is observed. Thus, the changes in fatty acid composition would take place in such a way that at least the average structural and viscotropic properties of the lipid phase of the membrane, sensed by these probes, would be almost exactly compensated.

[1]  E. Gratton,et al.  LIPID PHASES IN RENAL BRUSH BORDER MEMBRANES REVEALED BY LAURDAN FLUORESCENCE * , 1993, Photochemistry and photobiology.

[2]  E. London,et al.  Determination of the location of fluorescent probes attached to fatty acids using parallax analysis of fluorescence quenching: effect of carboxyl ionization state and environment on depth. , 1992, Biochemistry.

[3]  A. Schroit,et al.  Transbilayer movement of phospholipids in red cell and platelet membranes. , 1991, Biochimica et biophysica acta.

[4]  E Gratton,et al.  Orientational distribution of 1,6-diphenyl-1,3,5-hexatriene in phospholipid vesicles as determined by global analysis of frequency domain fluorimetry data. , 1991, Biochemistry.

[5]  C. Léger,et al.  Evidence for a structurally specific role of essential polyunsaturated fatty acids depending on their peculiar double-bond distribution in biomembranes. , 1990, Biochemistry.

[6]  C. Léger,et al.  Modification of fluidity and lipid-protein relationships in pig intestinal brush-border membrane by dietary essential fatty acid deficiency. , 1989, Biochimica et biophysica acta.

[7]  R. R. Brenner,et al.  The influence of fatty acid unsaturation and physical properties of microsomal membrane phospholipids on UDP-glucuronyltransferase activity. , 1989, Biochemical Journal.

[8]  Enrico Gratton,et al.  Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene in phospholipid vesicles. , 1987, Biochemistry.

[9]  P. Yeagle Cholesterol and the cell membrane. , 1985, Biochimica et biophysica acta.

[10]  H. Garda,et al.  In vitro modification of cholesterol content of rat liver microsomes. Effects upon membrane 'fluidity' and activities of glucose-6-phosphatase and fatty acid desaturation systems. , 1985, Biochimica et biophysica acta.

[11]  R. Dale,et al.  Transverse location of the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene in model lipid bilayer membrane systems by resonance excitation energy transfer. , 1985, Biochemistry.

[12]  J. Mead The non-eicosanoid functions of the essential fatty acids. , 1984, Journal of lipid research.

[13]  A. D. Smith,et al.  The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. , 1984, Biochimica et biophysica acta.

[14]  A. Kleinfeld,et al.  Interaction of fluorescence quenchers with the n-(9-anthroyloxy) fatty acid membrane probes , 1983 .

[15]  K. Kinosita,et al.  Effect of double bonds on the dynamic properties of the hydrocarbon region of lecithin bilayers. , 1981, Biochemistry.

[16]  J. Lakowicz,et al.  Differential polarized phase fluorometric investigations of diphenylhexatriene in lipid bilayers. Quantitation of hindered depolarizing rotations. , 1979, Biochemistry.

[17]  R. W. Evans,et al.  Monolayers of sterols and phosphatidylcholines containing a 20-carbon chain. , 1978, Chemistry and physics of lipids.

[18]  K. Kinosita,et al.  A theory of fluorescence polarization decay in membranes. , 1977, Biophysical journal.

[19]  G. Weber Theory of differential phase fluorometry: Detection of anisotropic molecular rotations , 1977 .

[20]  R. R. Brenner,et al.  Linoleic acid desaturation activity of liver microsomes of essential fatty acid deficient and sufficient rats. , 1976, Biochimica et biophysica acta.

[21]  J. Seelig,et al.  Bilayers of dipalmitoyl-3-sn-phosphatidylcholine. Conformational differences between the fatty acyl chains. , 1975, Biochimica et biophysica acta.

[22]  M. Schramm,et al.  Fatty acids as modulators of membrane functions: catecholamine-activated adenylate cyclase of the turkey erythrocyte. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[23]  F. Gunstone,et al.  Hydrocarbon chain packing and molecular motion in phospholipid bilayers formed from unsaturated lecithins. Synthesis and properties of sixteen positional isomers of 1,2-dioctadecenoyl-sn-glycero-3-phosphorylcholine. , 1975, The Journal of biological chemistry.

[24]  P. Hitchcock,et al.  Structural chemistry of 1,2 dilauroyl-DL-phosphatidylethanolamine: molecular conformation and intermolecular packing of phospholipids. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[25]  D. Ghosh,et al.  The influence of lecithin structure on their monolayer behavior and interactions with cholesterol. , 1973, Biochimica et biophysica acta.

[26]  L. Stryer,et al.  Fluorescent probes of biological membranes. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[27]  R. D. Spencer,et al.  Influence of Brownian Rotations and Energy Transfer upon the Measurements of Fluorescence Lifetime , 1970 .

[28]  W. Christie,et al.  Synthesis and characterization of the complete series of methylene-interrupted cis,cis-octadecadienoic acids , 1967 .

[29]  F. Gunstone,et al.  Fatty acids. Part 13. The synthesis of all the cis n-octadecenoic acids , 1967 .

[30]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[31]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[32]  C. Léger,et al.  Nutrition and biomembranes: additional information concerning the incidence of dietary polyunsaturated fatty acids on membrane organization and biological activity. , 1989, Biochimie.

[33]  J. East,et al.  Are essential fatty acids essential for membrane function? , 1986, Progress in lipid research.

[34]  J. Lakowicz Principles of fluorescence spectroscopy , 1983 .

[35]  H. Hansen Dietary essential fatty acids and in vivo prostaglandin production in mammals. , 1983, World review of nutrition and dietetics.

[36]  R. R. Brenner,et al.  Effect of temperature on the structure of rat liver microsomes studied by electron spin resonance, fluorescence and activity of enzymes involved in fatty acid biosynthesis. , 1980, Acta physiologica latino americana.

[37]  Mahendra K. Jain Introduction to biological membranes , 1980 .

[38]  R. R. Brenner,et al.  Metabolism of endogenous substrates by microsomes. , 1977, Drug metabolism reviews.