Disappearance of human [125I]iodochorionic gonadotropin from the circulation in the rat: tissue uptake and degradation.

125I-labeled hCG (human [125I]iodoCG) was injected iv into male and female rats, and the disappearance of the hormone from the circulation was studied. Also, the uptake of the hormone by different tissues, as well as its catabolic fate after the initial uptake were analyzed. The disappearance curve of human [125I]iodoCG consisted of two exponential components with apparent half-lives of 30 min and 4.8 h in both sexes. Two hours after the hormone injection, the uptake of radioactivity was 10.5% of the injected radioactivity in the kidneys, 2.2% in the liver, and 1.9% in the ovaries. A considerable amount of radioactivity, (1.5% of the total injected radioactivity) was also excreted in urine. Autoradiographs prepared from the kidney cortex and ovaries showed that renal uptake of the hormone took place mainly in the proximal tubule cells, and the ovarian uptake took place in the corpora lutea and theca cells. Gel filtration analyses of the radioactivity extracted with 0.5% Triton X-100 from the kidneys and l...

[1]  W. Neuman,et al.  The metabolism of labeled parathyroid hormone , 1975, Calcified Tissue Research.

[2]  H. Rajaniemi,et al.  Effect of radioiodination on the structural integrity, receptor and antibody-binding activity and circulatory behavior of human choriogonadotropin. , 1980, Hormone research.

[3]  M. Ascoli,et al.  Renal and hepatic lysosomal catabolism of luteinizing hormone , 1976, Molecular and Cellular Endocrinology.

[4]  M. Ascoli,et al.  The metabolism of luteinizing hormone. plasma clearance, urinary excretion, and tissue uptake , 1975, Molecular and Cellular Endocrinology.

[5]  H. Rajaniemi,et al.  Autoradiographic techniques for localizing protein hormones in target tissue. , 1975, Methods in enzymology.

[6]  H. Rajaniemi,et al.  Gonadotropin receptors in rat ovarian tissue. I. Localization of LH binding sites by fractionation of subcellular organelles. , 1974, Endocrinology.

[7]  L. Reichert,et al.  Progesterone production by dissociated rat ovarian cells: a sensitive method for quantitation of hormones with luteinizing activity. , 1974, The Journal of clinical endocrinology and metabolism.

[8]  T. Vanha-Perttula,et al.  Distribution of 125I-prolactin in mice and rats. Studies with whole-body and microautoradiography. , 1974, Hormone research.

[9]  R. Atkins,et al.  Role of the kidney in the metabolism of luteinizing hormone. , 1973, The Journal of endocrinology.

[10]  G. Mizejewski.  Tissue Distribution of Radioiodinated Human Growth Hormone in the Mouse 1 , 1973, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[11]  H. Papkoff.  The Chemistry of the Interstitial Cell-Stimulating Hormone of Ovine Pituitary Origin , 1973 .

[12]  T. Vanha-Perttula,et al.  Distribution of HCG and asialo-HCG in the mouse: quantitative and radioautographic studies. , 1973, Hormone research.

[13]  P. T. Varandani.  Insulin degradation. I. Purification and properties of glutathione-insulin transhydrogenase of rat liver. , 1972, Biochimica et biophysica acta.

[14]  A. Kitabchi,et al.  Characterization of a rat liver protease with specificity for insulin. , 1972, Endocrinology.

[15]  L. Reichert,et al.  Studies on the uptake of human chorionic gonadotropin and its subunits by rat testicular homogenates and interstitial tissue. , 1972, Endocrinology.

[16]  P. T. Varandani,et al.  Sequential degradation of insulin by rat liver homogenates. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[17]  A. Tashjian,et al.  Studies on the role of the liver in the metabolism of parathyroid hormone. I. Effects of partial hepatectomy and incubation of the hormone with tissue homogenates. , 1972, Endocrinology.

[18]  T. Vanha-Perttula,et al.  Specific receptor for LH in the ovary: evidence by autoradiography and tissue fractionation. , 1972, Endocrinology.

[19]  G. Ross,et al.  A method for the preparation of radioactive FSH with preservation of biologic activity. , 1971, Endocrinology.

[20]  G. Ross,et al.  Effects of progressive desialylation on the rate of disappearance of immunoreactive HCG from plasma in rats. , 1971, Endocrinology.

[21]  E. M. Bogdanove,et al.  Studies on the disappearance of LH and FSH in the rat; a quantitative approach to adenohypophysial secretory kinetics. , 1969, Endocrinology.

[22]  H. Burger,et al.  Radioautographic studies on the localization of 125I-labelled human luteinizing and growth hormone in immature male rats. , 1969, The Journal of endocrinology.

[23]  A. B. Maunsbach,et al.  The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. I. Comparison of different perfusion fixation methods and of glutaraldehyde, formaldehyde and osmium tetroxide fixatives. , 1966, Journal of ultrastructure research.

[24]  S. Fazakerley,et al.  SEPARATION OF AMINO ACIDS, AS COPPER CHELATES, FROM AMINO ACID, PROTEIN AND PEPTIDE MIXTURES. , 1965, Analytical biochemistry.

[25]  C. Janeway,et al.  Some isotopic studies on the distribution and metabolism of plasma proteins. , 1960, Advances in biological and medical physics.

[26]  C. Matthews,et al.  The theory of tracer experiments with 131I-labelled plasma proteins. , 1957, Physics in medicine and biology.

[27]  M. Goldner,et al.  Inactivation of insulin by the isolated liver of the bullfrog. , 1956, The American journal of physiology.

[28]  Mirsky Ia.  Insulinase, insulinase-inhibitors, and diabetes mellitus. , 1957 .

[29]  R. Wilson,et al.  Renal and extrarenal disposal of chorionic gonadotropin in the immediate postpartum period. , 1950, The Journal of clinical endocrinology and metabolism.

[30]  Mirsky Ia,et al.  The inactivation of insulin by tissue extracts; the effect of fasting on the insulinase content of rat liver. , 1949 .