Deoxyribonucleic Acid Polymerase Activities in Normal and Leukovirus-Infected Chicken Embryo Cells

Chicken embryo cells normally contain, in addition to deoxyribonucleic acid (DNA)-dependent DNA (D-DNA) polymerases, a novel “R-DNA-polymerase” which specifically copies polyriboadenylic acid strands. This R-DNA polymerase cannot copy natural ribonucleic acid or polyribocytidylic acid strands to a significant extent. Infection of cells with the leukovirus RAV-2 leads to the intracellular formation of large amounts of the viral RNA-dependent DNA polymerase whose properties differ from the cell R-DNA polymerase. Chicken cells transformed by a Rous sarcoma virus mutant which produce noninfectious alpha-type Rous sarcoma virus (f), a leukovirus known to be deficient in the viral RNA-dependent DNA polymerase, do not contain detectable viral RNA-dependent DNA polymerase, whereas the cellular R-DNA polymerase is found in normal amounts. There seems to be no relationship between the cellular R-DNA polymerase and the RNA-dependent DNA polymerase of the avian leukoviruses.

[1]  H. Hanafusa,et al.  Existence and expression of tumor virus genes in chick embryo cells. , 1972, Virology.

[2]  A. Weissbach,et al.  A new synthetic RNA-dependent DNA polymerase from human tissue culture cells (HeLa-fibroblast-synthetic oligonucleotides-template-purified enzymes). , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[3]  M. Baluda Widespread Presence, inChickens, ofDNA Complementary totheRNA GenomeofAvianLeukosis Viruses , 1972 .

[4]  H. Varmus,et al.  Detection of avian tumor virus-specific nucleotide sequences in avian cell DNAs (reassociation kinetics-RNA tumor viruses-gas antigen-Rous sarcoma virus, chick cells). , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[5]  R. Weiss,et al.  Induction of avian tumor viruses in normal cells by physical and chemical carcinogens. , 1971, Virology.

[6]  H. Hanafusa,et al.  DNA in uninfected and virus-infected cells complementary to avian tumor virus RNA. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[7]  A. Burny,et al.  Purification of the DNA polymerase of avian myeloblastosis virus. , 1971, Biochimica et biophysica acta.

[8]  S. Spiegelman,et al.  Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[9]  A. Weissbach,et al.  DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization. , 1971, Biochemical and biophysical research communications.

[10]  D. Baltimore,et al.  Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[11]  A. Weissbach,et al.  DNA polymerases from human cells. , 1971, Nature: New biology.

[12]  W. Robinson,et al.  DNA polymerase in defective Rous sarcoma virus. , 1971, Virology.

[13]  H. Hanafusa,et al.  Noninfectious RSV deficient in DNA polymerase. , 1971, Virology.

[14]  H. Hanafusa,et al.  Recovery of a new virus from apparently normal chick cells by infection with avian tumor viruses. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[15]  H. Hanafusa,et al.  A cell-associated factor essential for formation of an infectious form of Rous sarcoma virus. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[16]  S. Mizutani,et al.  RNA-dependent DNA polymerase in virions of Rous sarcoma virus. , 1970, Nature.

[17]  R. Weiss The Host Range of bryan Strain Rous Sarcoma Virus Synthesized in the Absence of Helper Virus , 1969 .

[18]  M. Sporn,et al.  A SIMPLE AND RAPID METHOD FOR THE ISOLATION OF ENZYMATICALLY ACTIVE HELA CELL NUCLEI , 1969, The Journal of cell biology.

[19]  L. N. Payne,et al.  Studies on the nature and genetic control of an antigen in normal chick embryos which reacts in the COFAL test. , 1968, Journal of General Virology.