E-cadherin expression in colorectal cancer. An immunocytochemical and in situ hybridization study.

Expression of the epithelial-specific adhesion molecule E-cadherin has been assessed in paraffin-embedded tissue from a series of 72 colorectal carcinomas. Using immunocytochemistry and in situ hybridization it was found that E-cadherin expression was related inversely to tumor differentiation. Out of 44 well- and moderately differentiated tumors, 36 expressed good positivity, whereas 24 of 28 poorly differentiated tumors were E-cadherin-negative. Classification by Dukes stage revealed a highly significant difference (P << 0.001) between A and B (32 positive, four negative) and C1 and C2 (seven positive, 29 negative) stages in terms of immunoreactivity. Of the 32 lymph node metastases studied, 20 were negative for E-cadherin expression, as were seven of eight liver metastases. These results indicate that the down-regulation of E-cadherin levels in vivo is associated with the dedifferentiation, progression, and metastasis of colorectal cancer.

[1]  W. Isaacs,et al.  Expression of the cellular adhesion molecule E-cadherin is reduced or absent in high-grade prostate cancer. , 1992, Cancer research.

[2]  B. Vogelstein,et al.  Antisense RNA to the putative tumor-suppressor gene DCC transforms Rat-1 fibroblasts. , 1992, Oncogene.

[3]  J. Thiery,et al.  Epithelial cell plasticity in neoplasia. , 1991, Cancer cells.

[4]  W. Birchmeier,et al.  E-cadherin expression in squamous cell carcinomas of head and neck: inverse correlation with tumor dedifferentiation and lymph node metastasis. , 1991, Cancer research.

[5]  Peter J. Bryant,et al.  The fat tumor suppressor gene in Drosophila encodes a novel member of the cadherin gene superfamily , 1991, Cell.

[6]  E. Gelmann,et al.  Cell adhesion molecule uvomorulin expression in human breast cancer cell lines: relationship to morphology and invasive capacities. , 1991, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.

[7]  W. Fiers,et al.  Genetic manipulation of E-cadherin expression by epithelial tumor cells reveals an invasion suppressor role , 1991, Cell.

[8]  Y. Doki,et al.  Expression of immunoreactive E-cadherin adhesion molecules in human cancers. , 1991, The American journal of pathology.

[9]  S. Hirohashi,et al.  Cadherin intercellular adhesion molecule in hepatocellular carcinomas: loss of E-cadherin expression in an undifferentiated carcinoma. , 1991, Cancer letters.

[10]  W. Fiers,et al.  Down‐regulation of E‐cadherin expression in madin darby canine kidney (MDCK) cells inside tumors of nude mice , 1991, International journal of cancer.

[11]  M. Takeichi,et al.  Cadherin cell adhesion receptors as a morphogenetic regulator. , 1991, Science.

[12]  A. Tanigami,et al.  Allelotype of breast cancer: cumulative allele losses promote tumor progression in primary breast cancer. , 1990, Cancer research.

[13]  S. Hirohashi,et al.  Allele loss on chromosome 16 associated with progression of human hepatocellular carcinoma. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Kathleen R. Cho,et al.  Identification of a chromosome 18q gene that is altered in colorectal cancers. , 1990, Science.

[15]  E. Ruoslahti,et al.  Integrins and tumor cell dissemination. , 1989, Cancer cells.

[16]  C. Damsky,et al.  Expression of the cell-cell adhesion glycoprotein cell-CAM 120/80 in normal human tissues and tumors. , 1989, The American journal of pathology.

[17]  Y. Takada,et al.  The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain , 1989, The Journal of cell biology.

[18]  W. Birchmeier,et al.  Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion , 1989, The Journal of cell biology.

[19]  A. Mansouri,et al.  Regional assignment of the human loci for uvomorulin (UVO) and chymotrypsinogen B (CTRB) with the help of two overlapping deletions on the long arm of chromosome 16. , 1989, Cytogenetics and cell genetics.

[20]  J. Varley,et al.  The localization of laminin mRNA and protein in the postimplantation embryo and placenta of the mouse: an in situ hybridization and immunocytochemical study. , 1988, Development.

[21]  Y. Nakamura,et al.  Genetic alterations during colorectal-tumor development. , 1988, The New England journal of medicine.

[22]  D. Vestweber,et al.  Identification of a putative cell adhesion domain of uvomorulin. , 1985, The EMBO journal.

[23]  W. Birchmeier,et al.  Dissociation of Madin-Darby canine kidney epithelial cells by the monoclonal antibody anti-arc-1: mechanistic aspects and identification of the antigen as a component related to uvomorulin , 1985, The Journal of cell biology.

[24]  D. R. Coman.  Decreased Mutual Adhesiveness, a Property of Cells from Squamous Cell Carcinomas , 1944 .

[25]  Phyllis,et al.  Human Actin Genes Are Single Copy for a-Skeletal and cx-Cardiac Actin but Multicopy for P-and y-Cytoskeletal Genes : 3 ' Untranslated Regions Are Isotype Specific but Are Conserved in Evolution , 2022 .