Carcinomas from rat liver cells transformed spontaneously in culture.

been on the formation of sarcomas from cells cultured in vitro by a variety of methods, but the formation of carcinomas as a result of the inoculation of untreated cells which have been in is seldom that long-term culture of mouse liver epithelium spontaneous transformation found spontaneous malignant transformation of several rat liver cell strains in culture; histological examination of tumors obtained from injection of 5 different strains derived from revealed sarcomas and carcinomas, except for 1 cell strain which produced "typical liver cell carcinoma." We report the production in rats of carcinomas derived from adult, Wistar liver cells in culture. An ampul containing cells 10% glycerol-90% plus