Cobitid Loach Following Ovulatory Stimulation

The time course change of 13,14-dihydro-l5-keto-prostaglandin F2ƒ¿ levels in holding waters of the cobitid loath, Misgurnus anguillicaudatus was studied during the ovulatory process initiated by an intraperitoneal injection of human chorionic gonadotropin (hCG) . The control fish were injected with Ringer's solution. The 13,14-dihydro-metabolite contents in the holding water were analyzed by high pressure liquid chromatography at 4 hour-intervals for 16 hours after the injections. In the non-ovulated status, levels of the 13,14-dihydro-metabolite were only barely detectable in the holding water even in the hCG-injected females. When the hCG-treated females ovulated, the release of the 13,14-dihydro metabolite reached maximum levels ranging from 6.71 to 14.38 ƒÊg/fish, tending to decrease thereafter. Levels of the 13,14-dihydro-metabolite were barely detectable in the holding water of the control fish through the experiment. Levels of prostaglandin F2ƒ¿ were also monitored and although individual release rates varied, a similar trend in release pattern was observed. Thus, the present results clearly show that in synchrony with ovulation, 13,14-dihydro-15-keto-prostaglandin F2ƒ¿ which would be one of the

[1]  J. Teeter,et al.  Sex pheromones of the sea lamprey (Petromyzon marinus): Steroid studies , 1987, Journal of Chemical Ecology.

[2]  R. Hurk,et al.  Gonadal steroidogenesis and the possible role of steroid glucuronides as sex pheromones in two species of teleosts , 1986, Fish Physiology and Biochemistry.

[3]  H. Ogata,et al.  Activities of F-type prostaglandins as releaser sex pheromones in cobitide loach, Misgurnus anguillicaudatus , 1994 .

[4]  H. Ogata,et al.  Olfactory responses of several species of teleost to F-prostaglandins. , 1994, Comparative biochemistry and physiology. Comparative physiology.

[5]  H. Ogata,et al.  F-Prostaglandins in the holding water of female loach , 1993 .

[6]  K. Kawabata,et al.  Sexual Behavior Induced by Amino Acids in the Rose Bitterling Rhodeus ocellatus ocellatus. , 1992 .

[7]  A. Moore,et al.  Testosterone is a potent odorant in precocious male Atlantic salmon (Salmo salar L.) parr , 1991 .

[8]  F. Goetz.  Compartmentalization of prostaglandin synthesis within the fish ovary. , 1991, The American journal of physiology.

[9]  A. Scott,et al.  Levels of steroids, including cortisol and 17α,20β-dihydroxy-4-pregnen-3-one, in plasma, seminal fluid, and urine of Pacific herring (Clupea hareng us pallasi) and North Sea plaice (Pleuronectes platessa L.) , 1991 .

[10]  F. Yamazaki.  The role of urine in sex discrimination in the goldfish Carassius auratus , 1990 .

[11]  R. Hurk,et al.  The chemical nature of sex attracting pheromones from the seminal vesicle of the African catfish, Clarias gariepinus , 1989 .

[12]  R. Peters,et al.  Steroid glucuronides of the seminal vesicle as olfactory stimuli in African catfish, Clarias gariepinus , 1989 .

[13]  F. Goetz,et al.  In vitro ovulation, prostaglandin synthesis, and proteolysis in isolated ovarian components of yellow perch (Perca flavescens): effects of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one and phorbol ester. , 1989, General and comparative endocrinology.

[14]  P. Sorensen,et al.  Direct evidence that 17α,20β-dihydroxy-4-pregnen-3-one functions as a goldfish primer pheromone: Preovulatory release is closely associated with male endocrine responses , 1989 .

[15]  F. Goetz,et al.  Prostaglandin F and E synthesis by specific tissue components of the brook trout (Salvelinus fontinalis) ovary , 1989 .

[16]  P. Sorensen,et al.  Periovulatory female goldfish release three potential pheromones: 17α,20β-Dihydroxyprogesterone, 17α,20β-dihydroxyprogesterone glucuronide, and 17α-hydroxyprogesterone , 1989 .

[17]  T. Murai,et al.  Changes in ammonia and amino acid levels in the erythrocytes and plasma of carp, Cyprinus carpio, during passage through the gills , 1988 .

[18]  W. Schoonen,et al.  Gas chromatographic-mass spectrometric analysis of steroids and steroid glucuronides in the seminal vesicle fluid of the African catfish, Clarias gariepinus. , 1987, General and comparative endocrinology.

[19]  R. van den Hurk,et al.  The biosynthesis of steroid glucuronides in the testis of the zebrafish, Brachydanio rerio, and their pheromonal function as ovulation inducers. , 1987, General and comparative endocrinology.

[20]  P. Sorensen,et al.  17α,20β-Dihydroxy-4-pregnen-3-one: a steroidal primer pheromone increasing milt volume in the goldfish, Carassius auratus , 1986 .

[21]  T. Kinoshita,et al.  Phorbol myristate acetate-stimulated release of cyclooxygenase products in rat pleural cells: derivatization of prostaglandins with 9-anthryldiazomethane for fluorometric determination by high performance liquid chromatography. , 1986, Prostaglandins.

[22]  P. Johansen.  Female pheromone and the behaviour of male guppies (Poecilia reticulata) in a temperature gradient , 1985 .

[23]  F. Goetz,et al.  Ovarian and plasma PGE and PGF levels in naturally ovulating brook trout (Salvelinus fontinalis) and the effects of indomethacin on prostaglandin levels. , 1983, Prostaglandins.

[24]  Susan Budavari,et al.  The Merck index : an encyclopedia of chemicals, drugs, and biologicals , 1983 .

[25]  E. Granström,et al.  Species differences in circulating prostaglandin metabolites. Relevance for the assay of prostaglandin release. , 1982, Biochimica et biophysica acta.

[26]  L. Colombo,et al.  Endocrinology of teleost reproduction: A testicular steroid pheromone in the black goby, Gobius jozo L. , 1980 .

[27]  N. Stacey,et al.  Regulation of Spawning Behaviour in the Female Goldfish , 1974, Nature.

[28]  J. Vane,et al.  Inactivation of Prostaglandins by the Lungs , 1970, Nature.