Monitoring the quality consistency of Fufang Danshen Pills using micellar electrokinetic chromatography fingerprint coupled with prediction of antioxidant activity and chemometrics.

A fast micellar electrokinetic chromatography fingerprint method combined with quantification was developed and validated to evaluate the quality of Fufang Danshen Pills, a traditional Chinese Medicine, which has been used in the treatment of cardiovascular system diseases, in which the tetrahedron optimization method was first used to optimize the background electrolyte solution. Subsequently, the index of the fingerprint information amount of I was performed as an excellent objective indictor to investigate the experimental conditions. In addition, a systematical quantified fingerprint method was constructed for evaluating the quality consistency of 20 batches of test samples obtained from the same drug manufacturer. The fingerprint analysis combined with quantitative determination of two components showed that the quality consistency of the test samples was quite good within the same commercial brand. Furthermore, the partial least squares model analysis was used to explore the fingerprint-efficacy relationship between active components and antioxidant activity in vitro, which can be applied for the assessment of anti-oxidant activity of Fufang Danshen pills and provide valuable medicinal information for quality control. The result illustrated that the present study provided a reliable and reasonable method for monitoring the quality consistency of Fufang Danshen pills.

[1]  Fei-Fei Gan,et al.  Antioxidant and Nrf2 inducing activities of luteolin, a flavonoid constituent in Ixeris sonchifolia Hance, provide neuroprotective effects against ischemia-induced cellular injury. , 2013, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[2]  X. Qin,et al.  Metabolic fingerprinting investigation of Tussilago farfara L. by GC–MS and multivariate data analysis , 2012 .

[3]  Ping Li,et al.  Quality assessment of traditional Chinese medicine herb couple by high-performance liquid chromatography and mass spectrometry combined with chemometrics. , 2016, Journal of separation science.

[4]  J. Coello,et al.  Enhanced chromatographic fingerprinting of herb materials by multi-wavelength selection and chemometrics. , 2012, Analytica chimica acta.

[5]  Zilin Chen,et al.  Tyrosinase inhibitor screening in traditional Chinese medicines by electrophoretically mediated microanalysis. , 2015, Journal of separation science.

[6]  A. Wojdyło,et al.  Antioxidant activity and phenolic compounds in 32 selected herbs , 2007 .

[7]  Simultaneous determination of galanthamine and lycorine in Lycoris radiata by a capillary electrophoresis with an electrochemiluminescence method. , 2014, Journal of separation science.

[8]  Guonan Chen,et al.  Capillary electrophoresis coupled with electrochemiluminescence detection for the separation and determination of thyreostatic drugs in animal feed. , 2014, Journal of separation science.

[9]  E. Wang,et al.  Simultaneous determination of two active ingredients in Flos daturae by capillary electrophoresis with electrochemiluminescence detection , 2005 .

[10]  R. Tauler,et al.  FTIR-ATR determination of solid non fat (SNF) in raw milk using PLS and SVM chemometric methods. , 2014, Food chemistry.

[11]  Wen Xu,et al.  A simple and sensitive HPLC method for the simultaneous determination of eight bioactive components and fingerprint analysis of Schisandra sphenanthera. , 2010, Analytica chimica acta.

[12]  Z. Su,et al.  Fingerprinting of Salvia miltiorrhiza Bunge by non-aqueous capillary electrophoresis compared with high-speed counter-current chromatography. , 2004, Journal of chromatography. A.

[13]  Patricia Sinnecker,et al.  Antioxidant activity of chlorophylls and their derivatives , 2005 .

[14]  Longshan Zhao,et al.  Evaluation of antioxidant activity of ten compounds in different tea samples by means of an on-line HPLC–DPPH assay , 2013 .

[15]  Yan Wang,et al.  Monitoring and Evaluating the Quality Consistency of Compound Bismuth Aluminate Tablets by a Simple Quantified Ratio Fingerprint Method Combined with Simultaneous Determination of Five Compounds and Correlated with Antioxidant Activities , 2015, PloS one.

[16]  F. Shahidi,et al.  Antioxidant and free radical scavenging activities of whole wheat and milling fractions , 2007 .

[17]  Merike Vaher,et al.  Fluorescence, electrophoretic and chromatographic fingerprints of herbal medicines and their comparative chemometric analysis. , 2015, Talanta.

[18]  Wen Zhao,et al.  Study of the determination and pharmacokinetics of Compound Danshen Dripping Pills in human serum by column switching liquid chromatography electrospray ion trap mass spectrometry. , 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[19]  Y. Heyden,et al.  Development, optimization and validation of a fingerprint of Ginkgo biloba extracts by high-performance liquid chromatography. , 2005, Journal of chromatography. A.

[20]  Yingjin Yuan,et al.  Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B. , 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[21]  A. Weston,et al.  Capillary electrophoresis methods development and sensitivity enhancement strategies for the separation of industrial and environmental chemicals , 1996 .

[22]  Kaixuan Xu,et al.  Separation and determination of flavonoids in three traditional chinese medicines by capillary electrophoresis with amperometric detection. , 2016, Journal of separation science.

[23]  Yan Wang,et al.  Capillary electrophoresis fingerprinting coupled with chemometrics to evaluate the quality consistency and predict the antioxidant activity of Sanhuang tablet as part of its quality control. , 2014, Journal of separation science.

[24]  Carmen Cruces-Blanco,et al.  Applications of capillary electrophoresis in forensic analytical chemistry , 2007 .

[25]  R. Pi,et al.  Tanshinone IIA protects cardiac myocytes against oxidative stress-triggered damage and apoptosis. , 2007, European journal of pharmacology.

[26]  C. Barbas,et al.  Capillary electrophoresis as a metabolomics tool for non-targeted fingerprinting of biological samples. , 2011, Journal of pharmaceutical and biomedical analysis.

[27]  Dao-quan Tang,et al.  Development of a novel method combining HPLC fingerprint and multi-ingredients quantitative analysis for quality evaluation of traditional Chinese medicine preparation. , 2011, Talanta.

[28]  G. Surányi,et al.  Quantification of main bioactive metabolites from saffron (Crocus sativus) stigmas by a micellar electrokinetic chromatographic (MEKC) method. , 2012, Journal of pharmaceutical and biomedical analysis.

[29]  Yi-Zeng Liang,et al.  Information theory applied to chromatographic fingerprint of herbal medicine for quality control. , 2003, Journal of chromatography. A.

[30]  Yu Wu,et al.  Multiple methods were combined to monitor and evaluate the quality of TCM, and make the results more reliable , 2014 .

[31]  R. Gotti.  Capillary electrophoresis of phytochemical substances in herbal drugs and medicinal plants. , 2011, Journal of pharmaceutical and biomedical analysis.

[32]  Yang Chu,et al.  The effect of Compound Danshen Dripping Pills, a Chinese herb medicine, on the pharmacokinetics and pharmacodynamics of warfarin in rats. , 2011, Journal of ethnopharmacology.

[33]  Guoxiang Sun,et al.  The overall quality control of Radix scutellariae by capillary electrophoresis fingerprint. , 2008, Journal of chromatographic science.

[34]  M. F. Silva,et al.  Comparative study between capillary electrophoresis and high performance liquid chromatography in 'guarana' based phytopharmaceuticals. , 2005, Journal of pharmaceutical and biomedical analysis.