[Effects of matrine on the relative molecules expression of proliferation and apoptosis in K562 cells].

OBJECTIVE To study the effects of matrine on proliferation and apoptosis of K562 cells. METHODS Cell culture and cell count, cell cycle phases and number of apoptotic cells were analyzed by flow cytometry. Morphology of apoptotic cells was studied by electron microscopy, BCL-6 and proliferating Cell nuclear antigen (PCNA) were checked by Western blot. RESULTS Matrine could inhibit the proliferation of K562 cells with inhibition rate of 69% after 72 hours matrine induction; number of K562 cells increased significantly in G1 phase, and number of apoptotic cell was 68% at 5th day of matrine treated cells; the typical morphology of apoptotic cells of K562 cells was observed by electron microscopy; BCL-6 was increased and PCNA was decreased in K562 cells induced by matrine. CONCLUSION Matrine inhibited the proliferation and induced the apoptosis of K562 cells significantly, the mechanism of which might be related to cell cycle arrest and the change of PCNA and BCL-6.