Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes.

The polymerase chain reaction was used to obtain randomly-amplified polymorphic DNA (RAPD) profiles from Listeria spp. and enterobacteria. Eleven different oligonucleotides were evaluated. Only one, HR4 (19mer), generated reproducible and specific profiles for Listeria spp., while results for enterobacteria were controversial. A total of 57 different Listeria strains were subjected to the RAPD analysis and 27 different profiles were recognized. RAPD typing allowed strains of the same serotype to be distinguished but the same profile was obtained from different serotypes of L. monocytogenes in three cases and in one case two different serotypes of L. innocua yielded the same profile. RAPD-typing with HR4 allowed L. monocytogenes contamination in several food outlets to be traced back to a food processing plant. In additional experiments, the general utility of this RAPD system in typing Yersinia enterocolitica, verotoxigenic Escherichia coli and Salmonella enteritidis was evaluated.

[1]  F. Baquero,et al.  Plasmids in Listeria. , 1982, Plasmid.

[2]  J. Rocourt,et al.  Différenciation biochimiquedes groupes génomiques de Listeria monocytogenes (sensu lato) , 1983 .

[3]  J. McLauchlin,et al.  The evaluation of a phage-typing system for Listeria monocytogenes for use in epidemiological studies. , 1986, Journal of medical microbiology.

[4]  B. Schwartz,et al.  Analysis of Listeria monocytogenes by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations. , 1989, International journal of food microbiology.

[5]  K. Livak,et al.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. , 1990, Nucleic acids research.

[6]  J. Welsh,et al.  Fingerprinting genomes using PCR with arbitrary primers. , 1990, Nucleic acids research.

[7]  A. Jenkins,et al.  DNA fingerprinting of isolates of Staphylococcus aureus from newborns and their contacts , 1991, Journal of clinical microbiology.

[8]  N. Pace,et al.  Detection of DNA contamination in Taq polymerase. , 1991, BioTechniques.

[9]  P. Boerlin,et al.  Typing of human, animal, food, and environmental isolates of Listeria monocytogenes by multilocus enzyme electrophoresis , 1991, Applied and environmental microbiology.

[10]  S. Harlander,et al.  Comparison of methods for discrimination between strains of Listeria monocytogenes from epidemiological surveys , 1991, Applied and environmental microbiology.

[11]  U. Candrian,et al.  Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragments. , 1991, The Journal of applied bacteriology.

[12]  K. Wernars,et al.  RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA , 1992, Letters in applied microbiology.

[13]  J. Rocourt,et al.  Use of rRNA gene restriction patterns for the identification of Listeria species , 1992 .

[14]  A. E. Lew,et al.  Restriction fragment length polymorphism analysis of Listeria monocytogenes and its application to epidemiological investigations. , 1992, International journal of food microbiology.

[15]  Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis , 1992, Applied and environmental microbiology.

[16]  A. Audurier,et al.  A comparative study of randomly amplified polymorphic DNA analysis and conventional phage typing for epidemiological studies of Listeria monocytogenes isolates. , 1992, Research in microbiology.

[17]  K. Wernars,et al.  Typing of Listeria strains by random amplification of polymorphic DNA. , 1992, Research in microbiology.

[18]  E. Wakeland,et al.  Concentration of primer and template qualitatively affects products in random-amplified polymorphic DNA PCR. , 1993, BioTechniques.

[19]  P. Desjardins,et al.  Molecular analysis of multiply recurrent meningitis due to Escherichia coli K1 in an infant. , 1993, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[20]  A. Gilmour,et al.  Development of a random amplification of polymorphic DNA typing method for Listeria monocytogenes , 1993, Applied and environmental microbiology.

[21]  G. Scoles,et al.  Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories. , 1993, PCR methods and applications.

[22]  G. McFeters,et al.  Distribution of bacteria within operating laboratory water purification systems , 1993, Applied and environmental microbiology.

[23]  C. Batt,et al.  Differentiation of Listeria monocytogenes and Listeria innocua by 16S rRNA genes and intraspecies discrimination of Listeria monocytogenes strains by random amplified polymorphic DNA polymorphisms , 1993, Applied and environmental microbiology.

[24]  J. Lüthy,et al.  Polymerase chain reaction (PCR): a possible alternative to immunochemical methods assuring safety and quality of food Detection of wheat contamination in non-wheat food products , 1993, Zeitschrift fur Lebensmittel-Untersuchung und -Forschung.