Microspectroscopy of single proliferating HeLa cells

Abstract Spectral differences between normal and abnormal tissue observed to date appear to be due to different averaging processes of spectral patterns that differ according to the cell's biochemistry, due to its state of maturation, differentiation, and development. Thus, disease perturbs the distribution of cells in the different stages of maturation, differentiation, and development. Previous FTIR microspectroscopic studies of normal versus neoplastic cells and tissues have demonstrated differences in the absorption intensities and band-shapes, particularly in the low frequency (1200–1000 cm −1 ) spectral region. In this study, we further investigated the spectral changes due to the drastic biochemical and morphological changes occurring as a consequence of cell proliferation.

[1]  E. Robbins,et al.  Cell synchronization. , 1974, Methods in enzymology.

[2]  C. Allis,et al.  Histone H3 phosphorylation is required for the initiation, but not maintenance, of mammalian chromosome condensation. , 1998, Journal of cell science.

[3]  Max Diem,et al.  Infrared Spectroscopy of Human Cells and Tissue: Detection of Disease , 2002, Technology in cancer research & treatment.

[4]  G. Merrill.  Chapter 13 Cell Synchronization , 1998 .

[5]  M. Diem,et al.  Infrared spectroscopy of human tissue. V. Infrared spectroscopic studies of myeloid leukemia (ML-1) cells at different phases of the cell cycle. , 1999, Biospectroscopy.

[6]  Mueller Gc.  Biochemical events in the animal cell cycle. , 1969 .

[7]  M. Diem,et al.  Infrared spectroscopy of human cells and tissue. VIII. Strategies for analysis of infrared tissue mapping data and applications to liver tissue. , 2000, Biopolymers.

[8]  Renato Baserga,et al.  The biology of cell reproduction , 1985 .

[9]  Max Diem,et al.  Raman and Infrared Microspectral Imaging of Mitotic Cells , 2006, Applied spectroscopy.

[10]  J. Aten,et al.  DNA double labelling with IdUrd and CldUrd for spatial and temporal analysis of cell proliferation and DNA replication , 1992, The Histochemical Journal.

[11]  T. A. Brown,et al.  Molecular biology labfax , 1991 .

[12]  Max Diem,et al.  IR spectroscopic studies of major cellular components. III. Hydration of protein, nucleic acid, and phospholipid films. , 2003, Biopolymers.

[13]  H. Mantsch,et al.  The use and misuse of FTIR spectroscopy in the determination of protein structure. , 1995, Critical reviews in biochemistry and molecular biology.

[14]  S. Vowler,et al.  A novel immunohistochemical method to estimate cell‐cycle phase distribution in archival tissue: implications for the prediction of outcome in colorectal cancer , 2003, The Journal of pathology.

[15]  L. Tolmach,et al.  Changes in X-ray Sensitivity of HeLa Cells during the Division Cycle , 1961, Nature.

[16]  H. Gratzner,et al.  Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. , 1982, Science.

[17]  James M. Roberts,et al.  Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family , 1991, Cell.

[18]  D. Wheatley,et al.  Cell growth and division , 1982 .

[19]  Max Diem,et al.  Infrared spectroscopy of cultured cells: II. Spectra of exponentially growing, serum-deprived and confluent cells , 2003 .

[20]  J. Decaprio,et al.  [7] Cell synchronization , 1995 .