Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization

We present analysis tools which are formulated using wide-field interferometric phase microscopy measurements, and show their ability to uniquely quantify the life cycle of live cancer cells. These parameters are based directly on the optical path delay profile of the sample and do not necessitate decoupling the refractive index and the thickness in the cell interferometric phase profile, and thus can be calculated using a single-frame acquisition. To demonstrate the use of these parameters, we have constructed a wide-field interferometric phase microscopy setup and closely traced the full lifecycle of HeLa cancer cells. These initial results show the potential of the parameters to distinguish between the different phases of the cell lifecycle, as well others biological phenomena.

[1]  Bahram Javidi,et al.  Cell Identification Computational 3-D Holographic Microscopy , 2011 .

[2]  Zhuo Wang,et al.  Optical measurement of cycle-dependent cell growth , 2011, Proceedings of the National Academy of Sciences.

[3]  P. Marquet,et al.  Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer , 2008, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[4]  Daniel Carl,et al.  Investigation of living pancreas tumor cells by digital holographic microscopy. , 2006, Journal of biomedical optics.

[5]  R. Porter,et al.  Allometry of mammalian cellular oxygen consumption , 2001, Cellular and Molecular Life Sciences CMLS.

[6]  Geoffrey M. Cooper.  The Events of M Phase , 2000 .

[7]  A. C. Burton,et al.  Distribution of Size and Shape in Populations of Normal Human Red Cells , 1968, Circulation research.

[8]  R J Kaufman.  Overview of vector design for mammalian gene expression. , 2000, Molecular biotechnology.

[9]  Gabriel Popescu,et al.  Erythrocyte structure and dynamics quantified by Hilbert phase microscopy. , 2005, Journal of biomedical optics.

[10]  Christian Depeursinge,et al.  Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy. , 2009, Journal of biomedical optics.

[11]  C. Fang-Yen,et al.  Tomographic phase microscopy , 2008, Nature Methods.

[12]  R. Brand,et al.  Quantification of nanoscale nuclear refractive index changes during the cell cycle. , 2011, Journal of biomedical optics.

[13]  H. Wadell,et al.  Volume, Shape, and Roundness of Quartz Particles , 1935, The Journal of Geology.

[14]  Zhuo Wang,et al.  Blood screening using diffraction phase cytometry. , 2010, Journal of biomedical optics.

[15]  Dennis C. Ghiglia,et al.  Two-Dimensional Phase Unwrapping: Theory, Algorithms, and Software , 1998 .

[16]  G. Popescu.  Quantitative phase imaging of nanoscale cell structure and dynamics. , 2008, Methods in cell biology.

[17]  J. Mitchison,et al.  The biology of the cell cycle , 1971 .

[18]  T. Kirchhausen,et al.  Mammalian Cells Change Volume during Mitosis , 2008, PloS one.

[19]  Natan T. Shaked,et al.  Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy , 2010, Biomedical optics express.

[20]  Farshid Guilak,et al.  Quantitative phase microscopy of articular chondrocyte dynamics by wide-field digital interferometry. , 2010, Journal of biomedical optics.

[21]  R. Barer.  Interference Microscopy and Mass Determination , 1952, Nature.

[22]  P N RAO,et al.  HeLa Cells: Effects of Temperature on the Life Cycle , 1965, Science.

[23]  E. Cuche,et al.  Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy. , 2005, Optics express.

[24]  K. Badizadegan,et al.  Live cell refractometry using microfluidic devices. , 2006, Optics letters.

[25]  T. W. Cooper,et al.  ELECTRON MICROSCOPE STUDIES OF THE MICROVILLI OF HELA CELLS , 1967, The Journal of cell biology.

[26]  Natan T. Shaked,et al.  Quantitative Analysis of Biological Cells Using Digital Holographic Microscopy , 2011 .

[27]  J. Weitzman,et al.  Growing without a size checkpoint , 2003, Journal of biology.

[28]  Patrik Langehanenberg,et al.  Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy. , 2010, Journal of biomedical optics.

[29]  E. Cuche,et al.  Cell refractive index tomography by digital holographic microscopy. , 2006, Optics letters.

[30]  J. Rogers,et al.  Spatial light interference microscopy (SLIM) , 2010, IEEE Photonic Society 24th Annual Meeting.

[31]  Gabriel Popescu,et al.  Optical imaging of cell mass and growth dynamics. , 2008, American journal of physiology. Cell physiology.