Design of elution strategy for simultaneous detection of chloramphenicol and gentamicin in complex samples using surface plasmon resonance.

For the analysis of massive samples containing multiple analytes, the enhancement of detection efficiency is crucial. In this study, a facile method was developed for sequential detection of chloramphenicol (CAP) and gentamicin (GEN) in complex samples, e.g. milk, using a surface plasmon resonance (SPR)-based biosensor. Based on the immune inhibition format, two conjugates of antigen and bovine serum albumin (BSA)-denoted as CAP-BSA and GEN-BSA-were grafted on the same channel of the SPR sensor chip. Two standard curves for CAP and GEN were separately obtained by first mixing a single antibody with different concentrations of the relevant antigen. Moreover, different regeneration solutions were screened for sequential analysis. An alkaline solution was found to completely remove the antibody against GEN (AbGEN) from the chip, but it exhibited limited ability to dissociate the antibody against CAP (AbCAP). Therefore, alkaline solution and Gly-HCl solutions are successively applied to elute AbGEN and AbCAP, respectively. By gradual elutions, CAP and GEN concentrations were simultaneously calculated with limit of detection values of 5.28ng/mL and 2.26ng/mL, respectively. Furthermore, the spiking milk samples with CAP and GEN validated the assay with recoveries of 77.6-101.1%. Therefore, this method is expected to improve the detection efficiency of SPR biosensors.

[1]  Laura M Lechuga,et al.  Highly sensitive dendrimer-based nanoplasmonic biosensor for drug allergy diagnosis. , 2015, Biosensors & bioelectronics.

[2]  You-Peng Chen,et al.  Surface plasmon resonance for water pollutant detection and water process analysis , 2016 .

[3]  M. Stobiecka,et al.  Modulation of Plasmon-Enhanced Resonance Energy Transfer to Gold Nanoparticles by Protein Survivin Channeled-Shell Gating. , 2015, The journal of physical chemistry. B.

[4]  R. Karlsson,et al.  Experimental design for kinetic analysis of protein-protein interactions with surface plasmon resonance biosensors. , 1997, Journal of immunological methods.

[5]  C. Bala,et al.  Surface Plasmon Resonance (SPR) Biosensors in Pharmaceutical Analysis , 2015, Critical reviews in analytical chemistry.

[6]  J. Mrázek,et al.  Low-fouling surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples. , 2016, Biosensors & bioelectronics.

[7]  M. Hämäläinen,et al.  Identification and optimization of regeneration conditions for affinity-based biosensor assays. A multivariate cocktail approach. , 1999, Analytical chemistry.

[8]  D. Myszka,et al.  Improving biosensor analysis , 1999, Journal of molecular recognition : JMR.

[9]  M. Stobiecka,et al.  Piezometric biosensors for anti-apoptotic protein survivin based on buried positive-potential barrier and immobilized monoclonal antibodies. , 2016, Biosensors & bioelectronics.

[10]  Qing Liu,et al.  Dual FITC lateral flow immunoassay for sensitive detection of Escherichia coli O157:H7 in food samples. , 2016, Biosensors & bioelectronics.

[11]  Magnus Malmqvist,et al.  Biospecific interaction analysis using biosensor technology , 1993, Nature.

[12]  J. Homola.  Present and future of surface plasmon resonance biosensors , 2003, Analytical and bioanalytical chemistry.

[13]  Cherie R. Kagan,et al.  At the Nexus of Food Security and Safety: Opportunities for Nanoscience and Nanotechnology. , 2016, ACS nano.

[14]  M. Stobiecka.  Novel plasmonic field-enhanced nanoassay for trace detection of proteins. , 2014, Biosensors & bioelectronics.

[15]  C. Min,et al.  Investigation of phase SPR biosensor for efficient targeted drug screening with high sensitivity and stability , 2015 .

[16]  Renliang Huang,et al.  Dopamine-assisted deposition and zwitteration of hyaluronic acid for the nanoscale fabrication of low-fouling surfaces. , 2016, Journal of materials chemistry. B.

[17]  Sehyun Shin,et al.  Ultrasensitive Detection of Single-Walled Carbon Nanotubes Using Surface Plasmon Resonance. , 2016, Analytical chemistry.

[18]  April Z Gu,et al.  SPRi-based adenovirus detection using a surrogate antibody method. , 2015, Biosensors & bioelectronics.

[19]  Pranveer Singh.  SPR Biosensors: Historical Perspectives and Current Challenges , 2016 .

[20]  Marek Piliarik,et al.  A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples. , 2010, Biosensors & bioelectronics.

[21]  Jing He,et al.  Development of a double-antibody sandwich ELISA for rapid detection of Bacillus Cereus in food , 2016, Scientific Reports.

[22]  A. Drake,et al.  A strategic and systematic approach for the determination of biosensor regeneration conditions. , 2011, Journal of immunological methods.

[23]  Yang Lu,et al.  Development of a surface plasmon resonance immunosensor for detecting melamine in milk products and pet foods. , 2014, Journal of agricultural and food chemistry.

[24]  W. Wong,et al.  Rapid testing methods for food contaminants and toxicants , 2015 .

[25]  J. Gooding,et al.  Development of sensitive direct and indirect enzyme-linked immunosorbent assays (ELISAs) for monitoring bisphenol-A in canned foods and beverages , 2012, Analytical and Bioanalytical Chemistry.

[26]  Zhimin He,et al.  Copper nanocluster-based fluorescent sensors for sensitive and selective detection of kojic acid in food stuff , 2014 .

[27]  Van Oss Cj.  Nature of specific ligand-receptor bonds, in particular the antigen-antibody bond. , 2000 .

[28]  Mihaela Puiu,et al.  SPR and SPR Imaging: Recent Trends in Developing Nanodevices for Detection and Real-Time Monitoring of Biomolecular Events , 2016, Sensors.

[29]  H. Mol,et al.  Determination of Glyphosate Levels in Breast Milk Samples from Germany by LC-MS/MS and GC-MS/MS. , 2016, Journal of agricultural and food chemistry.

[30]  W. Qi,et al.  Grafting hyaluronic acid onto gold surface to achieve low protein fouling in surface plasmon resonance biosensors. , 2014, ACS applied materials & interfaces.

[31]  H. Ho,et al.  Nanomaterials enhanced surface plasmon resonance for biological and chemical sensing applications. , 2014, Chemical Society reviews.

[32]  Yi Chen,et al.  Chemical Strategy to Stepwise Amplification of Signals in Surface Plasmon Resonance Imaging Detection of Saccharides and Glycoconjugates. , 2016, Analytical chemistry.

[33]  J. Homola.  Surface plasmon resonance sensors for detection of chemical and biological species. , 2008, Chemical reviews.

[34]  D. Myszka,et al.  Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors. , 1997, Current opinion in biotechnology.

[35]  Hongxia Chen,et al.  CB[7]-mediated signal amplification approach for sensitive surface plasmon resonance spectroscopy. , 2016, Biosensors & bioelectronics.

[36]  Günter Gauglitz,et al.  Surface plasmon resonance sensors: review , 1999 .

[37]  Zhimin He,et al.  A polydopamine-modified optical fiber SPR biosensor using electroless-plated gold films for immunoassays. , 2015, Biosensors & bioelectronics.