Antibacterial Properties of the Antimicrobial Peptide Gallic Acid-Polyphemusin I (GAPI)

A novel antimicrobial peptide, GAPI, has been developed recently by grafting gallic acid (GA) to polyphemusin I (PI). The objective of this study was to investigate the antibacterial effects of GAPI on common oral pathogens. This laboratory study used minimum inhibitory concentrations and minimum bactericidal concentrations to assess the antimicrobial properties of GAPI against common oral pathogens. Transmission electron microscopy was used to examine the bacterial morphology both before and after GAPI treatment. The results showed that the minimum inhibitory concentration ranged from 20 μM (Lactobacillus rhamnosus) to 320 μM (Porphyromonas gingivalis), whereas the minimum bactericidal concentration ranged from 80 μM (Lactobacillus acidophilus) to 640 μM (Actinomyces naeslundii, Enterococcus faecalis, and Porphyromonas gingivalis). Transmission electron microscopy showed abnormal curvature of cell membranes, irregular cell shapes, leakage of cytoplasmic content, and disruption of cytoplasmic membranes and cell walls. In conclusion, the GAPI antimicrobial peptide is antibacterial to common oral pathogens, with the potential to be used to manage oral infections.

[1]  Yuehua Wu,et al.  Burden, Trends, and Inequality of Dental Caries in the U.S., 1990-2019. , 2023, American journal of preventive medicine.

[2]  M. Mei,et al.  Bioactive Materials for Caries Management: A Literature Review , 2023, Dentistry journal.

[3]  M. Mei,et al.  Peptide Designs for Use in Caries Management: A Systematic Review , 2023, International journal of molecular sciences.

[4]  D. Tagliazucchi,et al.  Attenuation of Pseudomonas aeruginosa Virulence by Pomegranate Peel Extract , 2022, Microorganisms.

[5]  I. Korona-Głowniak,et al.  Streptococcus sobrinus as a Predominant Oral Bacteria Related to the Occurrence of Dental Caries in Polish Children at 12 Years Old , 2022, International journal of environmental research and public health.

[6]  M. Mei,et al.  Growing Global Research Interest in Antimicrobial Peptides for Caries Management: A Bibliometric Analysis , 2022, Journal of functional biomaterials.

[7]  Damita Cota,et al.  Antibacterial potential of ellagic acid and gallic acid against IBD bacterial isolates and cytotoxicity against colorectal cancer , 2022, Natural product research.

[8]  C. Chu,et al.  Tunnel Restoration: A Minimally Invasive Dentistry Practice , 2022, Clinical, cosmetic and investigational dentistry.

[9]  E. Blasi,et al.  Novel Options to Counteract Oral Biofilm Formation: In Vitro Evidence , 2022, International journal of environmental research and public health.

[10]  C. Ardila,et al.  Antibiotic resistance in periodontitis patients: a systematic scoping review of randomized clinical trials. , 2022, Oral diseases.

[11]  Yihong Li,et al.  Lactobacilli and human dental caries: more than mechanical retention , 2022, Microbiology.

[12]  C. Tonda-Turo,et al.  Antimicrobial peptide-based materials: opportunities and challenges. , 2022, Journal of materials chemistry. B.

[13]  M. Sweet,et al.  Modified horseshoe crab peptides target and kill bacteria inside host cells , 2021, Cellular and Molecular Life Sciences.

[14]  W. Wu,et al.  Efficacy of the dual-action GA-KR12 peptide for remineralising initial enamel caries: an in vitro study , 2021, Clinical Oral Investigations.

[15]  Z. Kesmen,et al.  Antimicrobial peptides (AMPs): A promising class of antimicrobial compounds , 2021, Journal of applied microbiology.

[16]  W. Y. Lam,et al.  Nonrestorative Management of Dental Caries , 2021, Dentistry journal.

[17]  T. Naas,et al.  Antimicrobial Peptides: A Potent Alternative to Antibiotics , 2021, Antibiotics.

[18]  T. Oh,et al.  Treatment of stage I-III periodontitis-The EFP S3 level clinical practice guideline , 2021, Journal of Evidence Based Dental Practice.

[19]  W. Wu,et al.  Remineralising dentine caries using an artificial antimicrobial peptide: an in vitro study. , 2021, Journal of dentistry.

[20]  W. Wu,et al.  Remineralising dentine caries using an artificial antimicrobial peptide: an in vitro study. , 2021, Journal of dentistry.

[21]  Xuedong Zhou,et al.  Inhibition of Streptococcus mutans biofilm formation by strategies targeting the metabolism of exopolysaccharides , 2021, Critical reviews in microbiology.

[22]  P. Jensen,et al.  A Novel Competence Pathway in the Oral Pathogen Streptococcus sobrinus , 2021, Journal of dental research.

[23]  T. Wolf,et al.  Antibacterial effect of silver diamine fluoride and potassium iodide against E. faecalis, A. naeslundii and P. micra , 2021, BMC oral health.

[24]  A. Uetanabaro,et al.  Anticariogenic activities of Libidibia ferrea, gallic acid and ethyl gallate against Streptococcus mutans in biofilm model. , 2021, Journal of ethnopharmacology.

[25]  W. Wu,et al.  The multifaceted roles of antimicrobial peptides in oral diseases. , 2021, Molecular oral microbiology.

[26]  W. Wu,et al.  Antimicrobial peptides for the prevention and treatment of dental caries: A concise review. , 2020, Archives of oral biology.

[27]  M. Mei,et al.  Effect of silver diamine fluoride on plaque microbiome in children. , 2020, Journal of dentistry.

[28]  J. Dickinson,et al.  Update on the adverse effects of antimicrobial therapies in community practice. , 2020, Canadian family physician Medecin de famille canadien.

[29]  M. Mahlapuu,et al.  Antimicrobial peptides as therapeutic agents: opportunities and challenges , 2020, Critical reviews in biotechnology.

[30]  M. Mei,et al.  Antibacterial effect of a new bioactive glass on cariogenic bacteria. , 2020, Archives of oral biology.

[31]  Andrew L. Ferguson,et al.  The value of antimicrobial peptides in the age of resistance. , 2020, The Lancet. Infectious diseases.

[32]  E. Daliri,et al.  Microbial Etiology and Prevention of Dental Caries: Exploiting Natural Products to Inhibit Cariogenic Biofilms , 2020, Pathogens.

[33]  I. Chapple,et al.  Treatment of stage I–III periodontitis—The EFP S3 level clinical practice guideline , 2020, Journal of clinical periodontology.

[34]  C. Ardila,et al.  Antimicrobial resistance of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tanerella forsythia in periodontitis patients. , 2020, Journal of global antimicrobial resistance.

[35]  F. Alghamdi,et al.  The Influence of Enterococcus faecalis as a Dental Root Canal Pathogen on Endodontic Treatment: A Systematic Review , 2020, Cureus.

[36]  Marilyn A. Anderson,et al.  Antimicrobial host defence peptides: functions and clinical potential , 2020, Nature Reviews Drug Discovery.

[37]  W. Qiu,et al.  Application of Antibiotics/Antimicrobial Agents on Dental Caries , 2020, BioMed research international.

[38]  D. Liang,et al.  Rational design of peptides with enhanced antimicrobial and anti‐biofilm activities against cariogenic bacterium Streptococcus mutans , 2019, Chemical biology & drug design.

[39]  Zhenhua Luo,et al.  Pathogenesis of Important Virulence Factors of Porphyromonas gingivalis via Toll-Like Receptors , 2019, Front. Cell. Infect. Microbiol..

[40]  Quan-yong He,et al.  The antimicrobial peptides and their potential clinical applications. , 2019, American journal of translational research.

[41]  Stefan Listl,et al.  Oral diseases: a global public health challenge , 2019, The Lancet.

[42]  Quanli Li,et al.  A tooth-binding antimicrobial peptide to prevent the formation of dental biofilm , 2019, Journal of Materials Science: Materials in Medicine.

[43]  A. Al-Ahmad,et al.  Resistance Toward Chlorhexidine in Oral Bacteria – Is There Cause for Concern? , 2019, Front. Microbiol..

[44]  S. Palmer,et al.  The Biology of Streptococcus mutans , 2019, Microbiology spectrum.

[45]  L. Moja,et al.  Analysis of the clinical antibacterial and antituberculosis pipeline. , 2019, The Lancet. Infectious diseases.

[46]  W. Lowman.  Minimum inhibitory concentration-guided antimicrobial therapy - the Achilles heel in the antimicrobial stewardship agenda. , 2018, South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde.

[47]  Jayachandran N. Kizhakkedathu,et al.  Antimicrobial Peptides: Diversity, Mechanism of Action and Strategies to Improve the Activity and Biocompatibility In Vivo , 2018, Biomolecules.

[48]  V. Ivanov,et al.  A Therapeutic Potential of Animal β-hairpin Antimicrobial Peptides. , 2017, Current medicinal chemistry.

[49]  C. Verma,et al.  Membrane Active Antimicrobial Peptides: Translating Mechanistic Insights to Design , 2017, Front. Neurosci..

[50]  T. Vos,et al.  Global, Regional, and National Prevalence, Incidence, and Disability-Adjusted Life Years for Oral Conditions for 195 Countries, 1990–2015: A Systematic Analysis for the Global Burden of Diseases, Injuries, and Risk Factors , 2017, Journal of dental research.

[51]  F. Hughes,et al.  Periodontitis‐associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14+ monocytes leading to enhanced Th17/IL‐17 responses , 2016, European journal of immunology.

[52]  Erin E. Gill,et al.  The immunology of host defence peptides: beyond antimicrobial activity , 2016, Nature Reviews Immunology.

[53]  D. Devine,et al.  Actinomyces spp. gene expression in root caries lesions , 2016, Journal of oral microbiology.

[54]  W. Marcenes,et al.  Global Economic Impact of Dental Diseases , 2015, Journal of dental research.

[55]  T. Karpiński,et al.  Chlorhexidine--pharmaco-biological activity and application. , 2015, European review for medical and pharmacological sciences.

[56]  H. Müller,et al.  Microbiology of aggressive periodontitis. , 2014, Periodontology 2000.

[57]  John J. Taylor.  Protein Biomarkers of Periodontitis in Saliva , 2014, ISRN inflammation.

[58]  M. V. van Hoek,et al.  The Human Cathelicidin Antimicrobial Peptide LL-37 as a Potential Treatment for Polymicrobial Infected Wounds , 2013, Front. Immunol..

[59]  J. Horbańczuk,et al.  Cathelicidins: family of antimicrobial peptides. A review , 2012, Molecular Biology Reports.

[60]  Christopher M Yip,et al.  Roles of Hydrophobicity and Charge Distribution of Cationic Antimicrobial Peptides in Peptide-Membrane Interactions* , 2012, The Journal of Biological Chemistry.

[61]  T. Silhavy,et al.  The bacterial cell envelope. , 2010, Cold Spring Harbor perspectives in biology.

[62]  H. Mayanagi,et al.  Transient acid-impairment of growth ability of oral Streptococcus, Actinomyces, and Lactobacillus: a possible ecological determinant in dental plaque. , 2009, Oral microbiology and immunology.

[63]  C. Vágvölgyi,et al.  The History of Alamethicin: A Review of the Most Extensively Studied Peptaibol , 2007, Chemistry & biodiversity.

[64]  M. Zehnder,et al.  Soft tissue dissolution capacity of currently used and potential endodontic irrigants. , 2004, Journal of endodontics.

[65]  M. Nascimento,et al.  Adaptive Acid Tolerance Response of Streptococcus sobrinus , 2004, Journal of bacteriology.

[66]  M. Fujimura,et al.  Purification, Characterization, and Sequencing of a Novel Type of Antimicrobial Peptides, Fa-AMP1 and Fa-AMP2, from Seeds of Buckwheat (Fagopyrum esculentum Moench.) , 2003, Bioscience, biotechnology, and biochemistry.

[67]  D. Wareham,et al.  99mTc-labeled antimicrobial peptides for detection of bacterial and Candida albicans infections. , 2002, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[68]  E. Pauwels,et al.  99mTc-labeled antimicrobial peptides for detection of bacterial and Candida albicans infections. , 2001, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[69]  H. Cang,et al.  Progress of Antimicrobial Discovery Against the Major Cariogenic Pathogen Streptococcus mutans. , 2019, Current issues in molecular biology.

[70]  G. Hajishengallis.  Immunomicrobial pathogenesis of periodontitis: keystones, pathobionts, and host response. , 2014, Trends in immunology.