Determination of Predominant Organic Acid Components in Malus Species: Correlation with Apple Domestication

Significant variation in organic acid components was detected in mature fruits of 101 apple accessions using high-performance liquid chromatography. The Malus species predominantly accumulated malic acid and citric acid, whereas wild fruits exhibited significantly higher levels of organic acid content than that in cultivated fruits. Differential accumulation patterns during fruit developmental stages was detected between malic acid and citric acid, thus suggesting a complex genetic regulation mechanism of organic acid metabolism in apple fruit. A highly positive correlation was detected between fruit total organic acid content with malic acid and citric acid content, thus suggesting that malic acid and citric acid are the principal determinants of apple fruit acidity. In contrast to malic acid, citric acid was predominantly detected in partial wild apples, while extremely low to undetectable concentrations of citric acid were observed in cultivated apple fruits; this is likely due to the genetic effects of parental characters. Our results provide vital information that could be useful for future studies on genetic analysis and improvement of organic acid accumulation in apple fruits.

[1]  S. Korban,et al.  Reduced representation genome sequencing reveals patterns of genetic diversity and selection in apple. , 2017, Journal of integrative plant biology.

[2]  S. Korban,et al.  Construction of a high density linkage map and its application in the identification of QTLs for soluble sugar and organic acid components in apple , 2016, Tree Genetics & Genomes.

[3]  J. Alonso-Prados,et al.  Development of a rapid and direct method for the determination of organic acids in peach fruit using LC-ESI-MS. , 2016, Food chemistry.

[4]  S. Korban,et al.  Genes Encoding Aluminum‐Activated Malate Transporter II and their Association with Fruit Acidity in Apple , 2015, The plant genome.

[5]  Zhenhai Han,et al.  A dense SNP genetic map constructed using restriction site-associated DNA sequencing enables detection of QTLs controlling apple fruit quality , 2015, BMC Genomics.

[6]  Yuepeng Han,et al.  Comparative assessment of sugar and malic acid composition in cultivated and wild apples. , 2015, Food chemistry.

[7]  M. Génard,et al.  What controls fleshy fruit acidity? A review of malate and citrate accumulation in fruit cells. , 2013, Journal of experimental botany.

[8]  H. Godoy,et al.  Validation of a HPLC method for simultaneous determination of main organic acids in fruits and juices , 2012 .

[9]  F. Stampar,et al.  Composition of sugars, organic acids, and total phenolics in 25 wild or cultivated berry species. , 2012, Journal of food science.

[10]  P. Flores,et al.  Determination of organic acids in fruits and vegetables by liquid chromatography with tandem-mass spectrometry , 2012 .

[11]  T. Giraud,et al.  New Insight into the History of Domesticated Apple: Secondary Contribution of the European Wild Apple to the Genome of Cultivated Varieties , 2012, PLoS genetics.

[12]  M. Boyce,et al.  Compositional Variation in Sugars and Organic Acids at Different Maturity Stages in Selected Small Fruits from Pakistan , 2012, International journal of molecular sciences.

[13]  Gaofeng Zhou,et al.  Identification of organic acid-related genes and their expression profiles in two pear (Pyrus pyrifolia) cultivars with difference in predominant acid type at fruit ripening stage , 2011 .

[14]  Roger E Bumgarner,et al.  The genome of the domesticated apple (Malus × domestica Borkh.) , 2010, Nature Genetics.

[15]  C. Ford,et al.  Regulation of malate metabolism in grape berry and other developing fruits. , 2009, Phytochemistry.

[16]  Faxing Chen,et al.  Developmental changes in pulp organic acid concentration and activities of acid-metabolising enzymes during the fruit development of two loquat (Eriobotrya japonica Lindl.) cultivars differing in fruit acidity , 2009 .

[17]  M. F. Drincovich,et al.  Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications. , 2009, Journal of experimental botany.

[18]  C. Richards,et al.  Genetic diversity and population structure in Malus sieversii, a wild progenitor species of domesticated apple , 2009, Tree Genetics & Genomes.

[19]  M. Maeshima,et al.  Vacuolar transporters and their essential role in plant metabolism. , 2006, Journal of experimental botany.

[20]  F. Stampar,et al.  Evaluation of peach and nectarine fruit quality and correlations between sensory and chemical attributes , 2005 .

[21]  G. Evanno,et al.  Detecting the number of clusters of individuals using the software structure: a simulation study , 2005, Molecular ecology.

[22]  H. Ross,et al.  Identification, cloning and expression analysis of strawberry (Fragaria x ananassa) mitochondrial citrate synthase and mitochondrial malate dehydrogenase. , 2004, Physiologia plantarum.

[23]  S. Harris,et al.  Genetic clues to the origin of the apple. , 2002, Trends in genetics : TIG.

[24]  P. Andrade,et al.  Study of the organic acids composition of quince (Cydonia oblonga Miller) fruit and jam. , 2002, Journal of agricultural and food chemistry.

[25]  L. Cohen,et al.  Comparative analysis of mitochondrial citrate synthase gene structure, transcript level and enzymatic activity in acidless and acid-containing Citrus varieties , 2001 .

[26]  A. Moing,et al.  Phosphoenolpyruvate carboxylase during grape berry development: protein level, enzyme activity and regulation , 2000 .

[27]  C. Givan.  Evolving concepts in plant glycolysis: two centuries of progress , 1999 .

[28]  H. Daood,et al.  Ion-Pair Chromatography and Photodiode-Array Detection of Vitamin C and Organic Acids , 1994 .

[29]  I. Mcmillan,et al.  THE STARCH IODINE TEST FOR DETERMINING STAGE OF MATURATION IN APPLES , 1979 .

[30]  Xiaosong Hu,et al.  Chemical compositional characterization of some apple cultivars , 2007 .