Characterization of Plasmid Deoxyribonucleic Acid from Neisseria gonorrhoeae

Five of six independent gonococcal isolates have been found to harbor a plasmid pool of covalently closed-circular deoxyribonucleic acid about 2.4 × 106 daltons in size. The plasmid species were 0.5-mol fraction of guanine plus cytosine and comprised 6 to 8% of the total gonococcal deoxyribonucleic acid equivalent, to approximately 24 to 32 copies per cell. There was no apparent correlation between the presence of these plasmid species and the in vitro clonal variation of gonococci associated with the loss of pili.

[1]  R. Finkelstein,et al.  Pathogenesis and Immunology of Experimental Gonococcal Infection: Virulence of Colony Types of Neisseria gonorrhoeae for Chicken Embryos , 1974 .

[2]  S. Falkow,et al.  General Method for the Isolation of Plasmid Deoxyribonucleic Acid , 1973, Journal of bacteriology.

[3]  J. Flynn,et al.  Survival of Neisseria gonorrhoeae in an artificial subcutaneous cavity of the mouse. , 1973, The British journal of venereal diseases.

[4]  A. Punsalang,et al.  Role of Pili in the Virulence of Neisseria gonorrhoeae , 1973, Infection and immunity.

[5]  N. Datta,et al.  Plasmids determining I pili constitute a compatibility complex. , 1973, Journal of general microbiology.

[6]  W. Sawyer,et al.  Studies on the Virulence of Neisseria gonorrhoeae I. Relation of Colonial Morphology and Resistance to Phagocytosis by Polymorphonuclear Leukocytes , 1973, Infection and immunity.

[7]  R. Clowes.  Molecular structure of bacterial plasmids , 1972, Bacteriological reviews.

[8]  A. Birch‐Andersen,et al.  Neisseria gonorrhoeae 3. Demonstration of presumed appendages to cells from different colony types. , 2009, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology.

[9]  E. Gotschlich,et al.  Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns. , 1971 .

[10]  S. Falkow,et al.  Polynucleotide Sequence Relationships Among Some Bacterial Plasmids , 1971, Journal of bacteriology.

[11]  J. De Ley.  Reexamination of the Association Between Melting Point, Buoyant Density, and Chemical Base Composition of Deoxyribonucleic Acid , 1970, Journal of bacteriology.

[12]  D. Kingsbury.  Estimate of the Genome Size of Various Microorganisms , 1969, Journal of bacteriology.

[13]  D. S. Kellogg,et al.  Neisseria gonorrhoeae II. Colonial Variation and Pathogenicity During 35 Months In Vitro , 1968, Journal of bacteriology.

[14]  D. Kingsbury,et al.  Use of exogenous adenine to label the nucleic acids of wild-type Neisseria meningitidis , 1967, Journal of bacteriology.

[15]  P. Sparling.  Genetic Transformation of Neisseria gonorrhoeae to Streptomycin Resistance , 1966, Journal of bacteriology.

[16]  E. Adelberg,et al.  Bacterial conjugation. , 1962, Annual review of microbiology.