Highly stable, amphiphilic DNA-encoded nanoparticle conjugates for DNA encoding/decoding applications

We synthesized highly stable, amphiphilic polymer-protected DNA–Au nanoparticle conjugates (DNA–AuNPs) and developed a DNA encoding/decoding strategy using these particles. Here, DNA–AuNPs were encapsulated by amphiphilic poly(N-vinyl-2-pyrrolidone) (PVP) to make them more stable and soluble in both organic and aqueous solvents. These particles are stable in a wide range of salt concentrations, pH and temperatures and sustain their stability even after the addition of D,L-dithiothreitol. Finally, this polymer layer was readily removed and particle-modified DNA was decoded via the microarrayed chip and dark-field-based AuNP imaging technique.

[1]  P. Yang,et al.  Crystal Growth , 2004 .

[2]  N. Kimizuka,et al.  One-pot room-temperature synthesis of single-crystalline gold nanocorolla in water. , 2009, Journal of the American Chemical Society.

[3]  J. Mu,et al.  A gold nanocomposite made soluble in both water and oil by the addition of a second adsorption layer of poly-N-vinyl-2-pyrrolidone on gold nanoparticles that have been made hydrophobic , 2006 .

[4]  Ruixia Chen,et al.  Degradation and degradation-induced re-assembly of PVP-PCL micelles. , 2010, Biomacromolecules.

[5]  R. V. Van Duyne,et al.  Detection of a biomarker for Alzheimer's disease from synthetic and clinical samples using a nanoscale optical biosensor. , 2005, Journal of the American Chemical Society.

[6]  Jin-Ho Choy,et al.  Inorganic–Biomolecular Hybrid Nanomaterials as a Genetic Molecular Code System , 2004 .

[7]  Fuyou Li,et al.  Photochromic response of nanoparticles of spiropyran–MnPS3 intercalate: A search for optically bistable nanocomponents , 2007 .

[8]  George C Schatz,et al.  What controls the melting properties of DNA-linked gold nanoparticle assemblies? , 2000, Journal of the American Chemical Society.

[9]  Xuchuan Jiang,et al.  Experiment and Theoretical Study of Poly(vinyl pyrrolidone)-controlled Gold Nanoparticles , 2008 .

[10]  C. Niemeyer,et al.  Sensitive detection of proteins using difunctional DNA-gold nanoparticles. , 2005, Small.

[11]  Tuan Vo-Dinh,et al.  Gold Nanostars For Surface-Enhanced Raman Scattering: Synthesis, Characterization and Optimization. , 2008, The journal of physical chemistry. C, Nanomaterials and interfaces.

[12]  C. Mirkin,et al.  Scanometric DNA array detection with nanoparticle probes. , 2000, Science.

[13]  Jinsheng Zheng,et al.  LSPR sensing of molecular biothiols based on noncoupled gold nanorods. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[14]  C. Mirkin,et al.  Multiple thiol-anchor capped DNA-gold nanoparticle conjugates. , 2002, Nucleic acids research.

[15]  Xiaojian Wang,et al.  Mechanisms of PVP in the preparation of silver nanoparticles , 2005 .

[16]  H. Shin,et al.  Mechanism of growth of colloidal silver nanoparticles stabilized by polyvinyl pyrrolidone in gamma-irradiated silver nitrate solution. , 2004, Journal of colloid and interface science.

[17]  Chad A. Mirkin,et al.  Oligonucleotide-Modified Gold Nanoparticles for Intracellular Gene Regulation , 2006, Science.

[18]  J. Storhoff,et al.  Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. , 1997, Science.

[19]  J. Nam,et al.  Lipid-gold-nanoparticle hybrid-based gene delivery. , 2008, Small.

[20]  Hui Li,et al.  Effect of pH on the Interaction of Gold Nanoparticles with DNA and Application in the Detection of Human p53 Gene Mutation , 2008, Nanoscale research letters.

[21]  K. Geckeler,et al.  Surfactant-directed multiple anisotropic gold nanostructures: synthesis and surface-enhanced Raman scattering. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[22]  S. An,et al.  Self-assembled arrays of zinc oxide nanoparticles from monolayer films of diblock copolymer micelles. , 2004, Chemical communications.

[23]  Z. Gu,et al.  Polymorphic structures of iodine and their phase transition in confined nanospace. , 2007, Nano letters.

[24]  Sung Yong Park,et al.  DNA-programmable nanoparticle crystallization , 2008, Nature.

[25]  Philip S Low,et al.  In vitro and in vivo two-photon luminescence imaging of single gold nanorods. , 2005, Proceedings of the National Academy of Sciences of the United States of America.