Desinfestação de sementes e multiplicação in vitro de guabijuzeiro a partir de segmentos apicais juvenis (Myrcianthes pungens O.Berg) D. Legrand

The guabijuzeiro is an evergreen tree 15-25 meters high. Occurs in Brazil from Sao Paulo to Rio Grande do Sul. Among the wild fruits this species has several characteristics which make it with potential for commercial use, in which the most important are related to fructification. Its propagation is carried out by seed and little information on vegetative propagation. Hence, the objective was to study the disinfection of seeds and in vitro multiplication of guabijuzeiro. The experiments were performed at the Laboratory of Biotechnology in Horticulture, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul. The test began with the disinfestation soaking in 70% ethanol for 1 minute, followed by a solution of sodium hypochlorite at concentrations of 0; 2; 4; 6 and 8%. The culture medium used in experiments was WPM with 30 g.L-1 sucrose and 7 g.L-1 of agar. For the testing of multiplication were used apical segments of seedlings from seeds germinated in vitro. The treatments consisted of BAP concentrations (0; 0,2; 0,4; 0,6; 0,8; 1 and 2 mg.L-1). As a result the concentrations between 4 and 6% sodium hypochlorite are more advantageous, as well as being effective in disinfestation, positively influence the germination. For multiplication, the cultivation of apical segments on medium with BAP at concentrations of up 1mg.l-1 was effective.