Measuring the Human Ultra-Weak Photon Emission Distribution Using an Electron-Multiplying, Charge-Coupled Device as a Sensor

Ultra-weak photon emission (UPE) is the spontaneous emission from living systems mainly attributed to oxidation reactions, in which reactive oxygen species (ROS) may play a major role. Given the capability of the next-generation electron-multiplying CCD (EMCCD) sensors and the easy use of liquid crystal tunable filters (LCTF), the aim of this work was to explore the potential of a simple UPE spectrometer to measure the UPE from a human hand. Thus, an easy setup was configured based on a dark box for inserting the subject’s hand prior to LCTF as a monochromator and an EMCCD sensor working in the full vertical binning mode (FVB) as a spectra detector. Under controlled conditions, both dark signals and left hand UPE were acquired by registering the UPE intensity at different selected wavelengths (400, 450, 500, 550, 600, 650, and 700 nm) during a period of 10 min each. Then, spurious signals were filtered out by ignoring the pixels whose values were clearly outside of the Gaussian distribution, and the dark signal was subtracted from the subject hand signal. The stepped spectrum with a peak of approximately 880 photons at 500 nm had a shape that agreed somewhat with previous reports, and agrees with previous UPE research that reported UPE from 420 to 570 nm, or 260 to 800 nm, with a range from 1 to 1000 photons s−1 cm−2. Obtaining the spectral distribution instead of the total intensity of the UPE represents a step forward in this field, as it may provide extra information about a subject’s personal states and relationship with ROS. A new generation of CCD sensors with lower dark signals, and spectrographs with a more uniform spectral transmittance, will open up new possibilities for configuring measuring systems in portable formats.

[1]  F. Popp Properties of biophotons and their theoretical implications. , 2003, Indian journal of experimental biology.

[2]  Wook Song,et al.  Effects of exercises on biophoton emission of the wrist , 2008, European Journal of Applied Physiology.

[3]  Masaki Kobayashi,et al.  Polychromatic spectral pattern analysis of ultra-weak photon emissions from a human body. , 2016, Journal of photochemistry and photobiology. B, Biology.

[4]  R. Prevedel,et al.  Direct detection of a single photon by humans , 2016, Nature Communications.

[5]  R. D. Lonati,et al.  Further measurements on the bioluminescence of the seedlings , 1955, Experientia.

[6]  F. Scholkmann,et al.  Non-chemical and non-contact cell-to-cell communication: a short review. , 2013, American journal of translational research.

[7]  I. Bókkon,et al.  Ultraweak photon emission in the brain. , 2015, Journal of integrative neuroscience.

[8]  R. Lüdtke,et al.  Differential effects of relaxation techniques on ultraweak photon emission. , 2008, Journal of alternative and complementary medicine.

[9]  T. Hankemeier,et al.  Measuring ultra-weak photon emission as a non-invasive diagnostic tool for detecting early-stage type 2 diabetes: A step toward personalized medicine. , 2017, Journal of photochemistry and photobiology. B, Biology.

[10]  T. Hankemeier,et al.  Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism , 2017, Scientific Reports.

[11]  Masaki Kobayashi,et al.  Highly sensitive imaging for ultra-weak photon emission from living organisms. , 2014, Journal of photochemistry and photobiology. B, Biology.

[12]  F A Popp,et al.  Biophoton emission of human body. , 1997, Indian journal of experimental biology.

[13]  R. Wijk,et al.  Multi-Site Recording and Spectral Analysis of Spontaneous Photon Emission from Human Body , 2005, Complementary Medicine Research.

[14]  Masaki Kobayashi,et al.  Imaging of Ultraweak Spontaneous Photon Emission from Human Body Displaying Diurnal Rhythm , 2009, PloS one.

[15]  M. Persinger,et al.  Increased photon emission from the head while imagining light in the dark is correlated with changes in electroencephalographic power: Support for Bókkon's biophoton hypothesis , 2012, Neuroscience Letters.

[16]  Huanming Yang,et al.  Ultra-weak photon emission of hands in aging prediction. , 2016, Journal of photochemistry and photobiology. B, Biology.

[17]  R. Wijk,et al.  Effect of Meditation on Ultraweak Photon Emission from Hands and Forehead , 2005, Complementary Medicine Research.

[19]  Anshu Rastogi,et al.  Spontaneous ultraweak photon emission imaging of oxidative metabolic processes in human skin: effect of molecular oxygen and antioxidant defense system. , 2011, Journal of biomedical optics.

[20]  Daniel Fels Correction: Cellular Communication through Light , 2009, PLoS ONE.

[21]  C. N. Scanaill,et al.  Sensor Technologies , 2014, Apress.

[22]  K. Soh,et al.  Year-long biophoton measurements: normalized frequency count analysis and seasonal dependency. , 2005, Journal of photochemistry and photobiology. B, Biology.

[23]  K. Soh,et al.  Left-right asymmetry of biophoton emission from hemiparesis patients. , 2003, Indian journal of experimental biology.

[24]  Jinxiang Han,et al.  Spectral discrimination between healthy people and cold patients using spontaneous photon emission. , 2015, Biomedical optics express.

[25]  T. Hankemeier,et al.  A Chinese literature overview on ultra-weak photon emission as promising technology for studying system-based diagnostics. , 2016, Complementary therapies in medicine.

[26]  J. van der Greef,et al.  Towards whole-body ultra-weak photon counting and imaging with a focus on human beings: a review. , 2014, Journal of photochemistry and photobiology. B, Biology.

[27]  Kwang-Sup Soh,et al.  Biophoton Emission from the Hands , 2002 .

[28]  S. Lamponi,et al.  New perspective in cell communication: potential role of ultra-weak photon emission. , 2014, Journal of photochemistry and photobiology. B, Biology.

[29]  M. Ashley,et al.  Making sense of sensitivity. , 2013, Dental update.

[30]  T. Hankemeier,et al.  Cellular glutathione levels in HL-60 cells during respiratory burst are not correlated with ultra-weak photon emission. , 2017, Journal of photochemistry and photobiology. B, Biology.

[31]  F. Stäb,et al.  Ultraweak photon emission of human skin in vivo: influence of topically applied antioxidants on human skin. , 1999, Methods in enzymology.

[32]  M. A. Nematollahi,et al.  Detection of Ultraweak Photon Emission (UPE) from Cells as a Tool for Pathological Studies , 2017, Journal of biomedical physics & engineering.

[33]  Daniel Fels,et al.  Cellular Communication through Light , 2009, PloS one.

[34]  O. S. Heavens,et al.  New Developments and Applications in Optical Radiometry , 1990 .

[35]  Endogenous physical regulation of population density in the freshwater protozoan Paramecium caudatum , 2017, Scientific Reports.

[36]  H. Schwabl,et al.  Spontaneous Ultraweak Photon Emission from Biological Systems and the Endogenous Light Field , 2005, Complementary Medicine Research.

[37]  M. Hiramatsu,et al.  Ultra-weak photon emission from human hand: influence of temperature and oxygen concentration on emission. , 2005, Journal of photochemistry and photobiology. B, Biology.

[38]  P. Pospíšil,et al.  Two‐dimensional imaging of spontaneous ultra‐weak photon emission from the human skin: role of reactive oxygen species , 2011, Journal of biophotonics.

[39]  P. Pospíšil,et al.  Role of reactive oxygen species in ultra-weak photon emission in biological systems. , 2014, Journal of photochemistry and photobiology. B, Biology.

[40]  R. Wijk,et al.  Spontaneous Ultra-Weak Photon Emission from Human Hands Is Time Dependent , 2007 .