Measurement of optical properties of biological tissues by low-coherence reflectometry.

We show that optical properties of dense biological tissues can be determined from backscattered power curves measured by a low-coherence reflectometer. Our measurement approach is based on a first-order scattering theory that relates the backscattered power to the total and backscattering cross sections of scatterers in a turbid medium. As a validation of the technique, measurements were made with a commercially available reflectometer on suspensions of polystyrene microspheres having known optical properties. With this reflectometer, which employs a 1300-nm LED source that emits less than 20 µW, we found that skin tissues could be probed to a depth of nearly 1 mm. Estimates of optical coefficients of human dermis and of a variety of excised animal tissues are given.

[1]  John,et al.  Diffusing-wave spectroscopy and multiple scattering of light in correlated random media. , 1989, Physical review. B, Condensed matter.

[2]  M S Patterson,et al.  Total attenuation coefficients and scattering phase functions of tissues and phantom materials at 633 nm. , 1987, Medical physics.

[3]  M S Patterson,et al.  Optical properties of normal and diseased human breast tissues in the visible and near infrared. , 1990, Physics in medicine and biology.

[4]  J. Fujimoto,et al.  Polarization-sensitive low-coherence reflectometer for birefringence characterization and ranging , 1992 .

[5]  R R Alfano,et al.  Imaging objects hidden in highly scattering media using femtosecond second-harmonic-generation cross-correlation time gating. , 1991, Optics letters.

[6]  J. Schmitt,et al.  Use of polarized light to discriminate short-path photons in a multiply scattering medium. , 1992, Applied optics.

[7]  S L Jacques,et al.  Optical properties of rat liver between 350 and 2200 nm. , 1989, Applied optics.

[8]  R Marchesini,et al.  Extinction and absorption coefficients and scattering phase functions of human tissues in vitro. , 1989, Applied optics.

[9]  H. Inaba,et al.  Two-dimensional coherent detection imaging in multiple scattering media based on the directional resolution capability of the optical heterodyne method , 1991 .

[10]  E. V. Browell,et al.  First- and second-order backscattering from clouds illuminated by finite beams. , 1972, Applied optics.

[11]  John M. Reid,et al.  Analysis and measurement of ultrasound backscattering from an ensemble of scatterers excited by sine‐wave bursts , 1973 .

[12]  J T Whitton,et al.  The thickness of the epidermis , 1973, The British journal of dermatology.

[13]  B L Danielson,et al.  Guided-wave reflectometry with micrometer resolution. , 1987, Applied optics.

[14]  K. Takada,et al.  New measurement system for fault location in optical waveguide devices based on an interferometric technique. , 1987, Applied optics.

[15]  J. Fujimoto,et al.  Micron‐resolution ranging of cornea anterior chamber by optical reflectometry , 1991, Lasers in surgery and medicine.

[16]  M S Patterson,et al.  INDIRECT VERSUS DIRECT TECHNIQUES FOR THE MEASUREMENT OF THE OPTICAL PROPERTIES OF TISSUES , 1987, Photochemistry and photobiology.

[17]  R. Anderson,et al.  The optics of human skin. , 1981, The Journal of investigative dermatology.

[18]  F. P. Bolin,et al.  Refractive index of some mammalian tissues using a fiber optic cladding method. , 1989, Applied optics.

[19]  Robert R. Alfano,et al.  Biological materials probed by the temporal and angular profiles of the backscattered ultrafast laser pulses , 1990 .

[20]  R R Alfano,et al.  Determination of the scattering and absorption lengths from the temporal profile of a backscattered pulse. , 1990, Optics letters.

[21]  L. Grossweiner,et al.  Applications of the 1-D diffusion approximation to the optics of tissues and tissue phantoms. , 1989, Applied optics.

[22]  A. Welch,et al.  A review of the optical properties of biological tissues , 1990 .