Transmission digital holographic microscopy based on a beam-splitter cube interferometer.

A new optical configuration for digital holographic microscopy is presented. Digital off-axis holograms are recorded by use of a single cube beam splitter in a nonconventional configuration to both split and combine a diverging spherical wavefront as it emerges from a single point source. Both the amplitude and the phase can then be reconstructed, yielding intensity and phase images with improved resolution. The novelty of the proposed configuration is its simplicity, minimal number of optical elements, insensitivity to vibration, and its inherent capability to compensate for the phase curvature that results from the illuminating wavefront in the case of microscopic samples.

[1]  U. Schnars.  Direct phase determination in hologram interferometry with use of digitally recorded holograms , 1994 .

[2]  Ichirou Yamaguchi,et al.  Phase-shifting digital holography , 1997 .

[3]  E. Cuche,et al.  Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. , 1999, Applied optics.

[4]  E. Cuche,et al.  Digital holography for quantitative phase-contrast imaging. , 1999, Optics letters.

[5]  Werner Jüptner,et al.  Digital recording and numerical reconstruction of holograms , 2002 .

[6]  Chun-Min Lo,et al.  High-resolution quantitative phase-contrast microscopy by digital holography. , 2005, Optics express.

[7]  Christian Depeursinge,et al.  Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram. , 2006, Optics express.

[8]  Liren Liu,et al.  Ridge-shape phase distribution adjacent to 180° domain wall in congruent LiNbO3 crystal , 2006 .

[9]  Etienne Cuche,et al.  Purely numerical compensation for microscope objective phase curvature in digital holographic microscopy: influence of digital phase mask position. , 2006, Journal of the Optical Society of America. A, Optics, image science, and vision.

[10]  Etienne Cuche,et al.  Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation. , 2006, Applied optics.

[11]  Etienne Cuche,et al.  Numerical parametric lens for shifting, magnification, and complete aberration compensation in digital holographic microscopy. , 2006, Journal of the Optical Society of America. A, Optics, image science, and vision.

[12]  José A. Ferrari,et al.  Single-element interferometer , 2007 .