Rapid biomimetic synthesis of silver nanoparticles from asiatic mangrove (Rhizopora mucronata) and its antimicrobial activity against clinically isolated pathogens

In the present study, a facile and ecofriendly method has been developed for the synthesis of silver nanoparticles from silver nitrate using Asiatic mangrove (Rhizopora mucronata) a natural biopolymer, as a reducing and stabilizing agent. The Influence of different parameters such as pH, temperature and reaction time on the synthesis of nanoparticles was studied. The results recorded from UV-vis spectrum, scanning electron microscopy (SEM), Energy Dispersive Spectroscopy (EDS), X-ray diffraction (XRD) and Atomic Force Microscopy (AFM) support the biosynthesis and characterization of silver nanoparticles. Biosynthesis of silver nanoparticles and activity on clinically isolated human pathogens viz., Gram positive and Gram negative bacteria and fungi were investigated. The synthesized silver nanoparticles had significant antibacterial action on both the classes of bacteria. As the silver nanoparticles are encapsulated with functional group rich mangrove, they can be easily integrated for various medical applications.

[1]  F. Caruso,et al.  Characterization of Polyelectrolyte-Protein Multilayer Films by Atomic Force Microscopy, Scanning Electron Microscopy, and Fourier Transform Infrared Reflection-Absorption Spectroscopy. , 1998 .

[2]  S. Nie,et al.  Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules , 2001, Nature Biotechnology.

[3]  Absar Ahmad,et al.  Role of halide ions and temperature on the morphology of biologically synthesized gold nanotriangles. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[4]  Absar Ahmad,et al.  Synthesis of triangular Au core-Ag shell nanoparticles , 2007 .

[5]  Kriengsak Lirdprapamongkol,et al.  Eco-Friendly Synthesis of Fucoidan-Stabilized Gold Nanoparticles , 2010 .

[6]  Beom Soo Kim,et al.  Rapid biological synthesis of silver nanoparticles using plant leaf extracts , 2009, Bioprocess and biosystems engineering.

[7]  Christopher M. Eastman,et al.  A simple route for manufacturing highly dispersed silver nanoparticles , 2007 .

[8]  P. Selvakumar,et al.  Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. , 2010, Colloids and surfaces. B, Biointerfaces.

[9]  Xuesi Chen,et al.  Biodegradable electrospun poly(l-lactide) fibers containing antibacterial silver nanoparticles , 2006 .

[10]  Absar Ahmad,et al.  Synthesis of Gold Nanotriangles and Silver Nanoparticles Using Aloevera Plant Extract , 2006, Biotechnology progress.

[11]  Sudesh Kumar Yadav,et al.  Biosynthesis of nanoparticles: technological concepts and future applications , 2008 .

[12]  Yinglei Xu,et al.  Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions , 2009 .

[13]  S. Dhara,et al.  Electrical transport studies of Ag nanoclusters embedded in glass matrix , 2001 .

[14]  Tetsuya Osaka,et al.  THE STUDY OF ANTIMICROBIAL ACTIVITY AND PRESERVATIVE EFFECTS OF NANOSILVER INGREDIENT , 2005 .

[15]  V. Sharma,et al.  Silver nanoparticles: green synthesis and their antimicrobial activities. , 2009, Advances in colloid and interface science.

[16]  M. Brust,et al.  Self-Assembled Gold Nanoparticle Thin Films with Nonmetallic Optical and Electronic Properties , 1998 .

[17]  Absar Ahmad,et al.  Biosynthesis of gold and silver nanoparticles using Emblica Officinalis fruit extract, their phase transfer and transmetallation in an organic solution. , 2005, Journal of nanoscience and nanotechnology.

[18]  S. Saxena,et al.  BIOINSPIRED SYNTHESIS OF SILVER NANOPARTICLES , 2009 .

[19]  Shiv Shankar,et al.  Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes , 2003 .

[20]  Christopher J. Kiely,et al.  Some recent advances in nanostructure preparation from gold and silver particles: a short topical review , 2002 .

[21]  Andreas Kornowski,et al.  Determination of nanocrystal sizes: a comparison of TEM, SAXS, and XRD studies of highly monodisperse CoPt3 particles. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[22]  Spectroscopic characterization of gold nanoparticles formed by cells and S-layer protein of Bacillus sphaericus JG-A12 , 2007 .

[23]  M. Sastry,et al.  Gold Nanotriangles Biologically Synthesized using Tamarind Leaf Extract and Potential Application in Vapor Sensing , 2005 .

[24]  Ajay Misra,et al.  GREEN SYNTHESIS OF SILVER NANOPARTICLES USING LATEX OF JATROPHA CURCAS , 2009 .

[25]  Absar Ahmad,et al.  Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. , 2004, Journal of colloid and interface science.

[26]  V. S. Kumar,et al.  Highly efficient Ag/C catalyst prepared by electro-chemical deposition method in controlling microorganisms in water , 2004 .

[27]  B. Sreedhar,et al.  Preparation of acacia‐stabilized silver nanoparticles: A green approach , 2007 .

[28]  M. Camacho-López,et al.  Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract , 2008 .

[29]  M. Yacamán,et al.  Synthesis of Carbon Onions by Gold Nanoparticles and Electron Irradiation , 2003 .

[30]  Balaprasad Ankamwar,et al.  Biological synthesis of triangular gold nanoprisms , 2004, Nature materials.

[31]  M. Kowshik,et al.  Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3 , 2002 .

[32]  Absar Ahmad,et al.  Geranium Leaf Assisted Biosynthesis of Silver Nanoparticles , 2003, Biotechnology progress.