Pooling and PCR as a method to combat low frequency gene targeting in mouse embryonic stem cells

[1]  B. Martoglio,et al.  Identification of Signal Peptide Peptidase, a Presenilin-Type Aspartic Protease , 2002, Science.

[2]  R. Sarig,et al.  Increased efficiency of homologous recombination in ES cells by cleavage at both ends of homology in the targeting vector , 2000, Transgenic Research.

[3]  P. Paz,et al.  Minors held by majors: the H13 minor histocompatibility locus defined as a peptide/MHC class I complex. , 1997, Immunity.

[4]  B. Safer,et al.  Efficient gene targeting in mouse embryonic stem cells , 1997, Gene Therapy.

[5]  D. Wells,et al.  ES cell cycle rates affect gene targeting frequencies. , 1997, Experimental cell research.

[6]  M. Gottesman,et al.  Construction of a new universal vector for insertional mutagenesis by homologous recombination. , 1992, Gene.

[7]  B. Wieringa,et al.  Targeting of the creatine kinase M gene in embryonic stem cells using isogenic and nonisogenic vectors. , 1992, Nucleic acids research.

[8]  M. Capecchi,et al.  Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus , 1992, Molecular and cellular biology.

[9]  S. Beverley,et al.  Double targeted gene replacement for creating null mutants. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[10]  J. Seidman,et al.  Embryonic stem cells lacking a functional inhibitory G-protein subunit (alpha i2) produced by gene targeting of both alleles. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[11]  T. Yagi,et al.  Homologous recombination at c-fyn locus of mouse embryonic stem cells with use of diphtheria toxin A-fragment gene in negative selection. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[12]  H. S. Kim,et al.  Recombinant fragment assay for gene targetting based on the polymerase chain reaction. , 1988, Nucleic acids research.

[13]  M. Capecchi,et al.  Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells , 1987, Cell.

[14]  T. Dechiara.  Gene targeting in ES cells. , 2001, Methods in molecular biology.

[15]  L. Tessarollo.  Manipulating mouse embryonic stem cells. , 2001, Methods in molecular biology.

[16]  A. Zimmer.  BY HOMOLOGOUS RECOMBINATION IN EMBRYONIC STEM CELLS , 1992 .