Micro-structured surfaces for algal biofilm growth

It is well known that cells respond to structured surface cues that are on the micro/nanometer scale. Tissue engineering and bio-fouling fields have utilized the semiconductor device fabrication processes to make micro- and nanometer patterned surfaces to study animal cell tissue formation and to prevent algae attachment on marine surfaces respectively. In this paper we describe the use of micro-structured surfaces to study the attachment and growth of algal films. This paper gives an overview of how micro-structured surfaces are made for this purpose, how they are incorporated into a photo bioreactor and how this patterning influences the growth of an algal biofilm. Our results suggest that surface patterning with deeper V-groove patterns that are of the same size scale as the algal species has resulted in higher biomass productivity giving them a chance to embed and attach on the slope and flat surfaces whereas shallower size grooves and completely flat surfaces did not show this trend.

[1]  Antonio Nanci,et al.  Surface Nanopatterning to Control Cell Growth , 2008 .

[2]  C. Wilkinson,et al.  Topographical control of cell behaviour: II. Multiple grooved substrata. , 1990, Development.

[3]  Yingxiao Wang,et al.  Micro/nano-fabrication technologies for cell biology , 2010, Medical & Biological Engineering & Computing.

[4]  I. Priyadarshani,et al.  Commercial and industrial applications of micro algae - A review , 2012 .

[5]  D. Kern,et al.  Influence of surface patterning on bacterial growth behavior , 2011 .

[6]  J. Grover,et al.  Micro-scale surface-patterning influences biofilm formation , 2009 .

[7]  Steven L. Percival,et al.  Biofilms and Veterinary Medicine , 2011 .

[8]  C J Murphy,et al.  Effects of synthetic micro- and nano-structured surfaces on cell behavior. , 1999, Biomaterials.

[9]  Charles L. Thomas,et al.  Rapid tooling using SU-8 for injection molding microfluidic components , 2000, SPIE MOEMS-MEMS.

[10]  Joanna Aizenberg,et al.  Fine-tuning the degree of stem cell polarization and alignment on ordered arrays of high-aspect-ratio nanopillars. , 2012, ACS nano.

[11]  H. Flemming,et al.  The biofilm matrix , 2010, Nature Reviews Microbiology.

[12]  D. G. Allen,et al.  Algae biofilm growth and the potential to stimulate lipid accumulation through nutrient starvation. , 2013, Bioresource technology.

[13]  Michael Kröger,et al.  Review on possible algal-biofuel production processes , 2012 .

[14]  Chang‐Hwan Choi,et al.  Advanced Nanostructured Surfaces for the Control of Biofouling: Cell Adhesions to Three-Dimensional Nanostructures , 2012 .

[15]  T. Irving Factors Influencing the Formation and Development of Microalgal Biofilms , 2011 .

[16]  P Connolly,et al.  Cell guidance by ultrafine topography in vitro. , 1991, Journal of cell science.

[17]  C. Wilkinson,et al.  Applications of nano-patterning to tissue engineering , 2006 .

[18]  D. G. Allen,et al.  Species and material considerations in the formation and development of microalgal biofilms , 2011, Applied Microbiology and Biotechnology.

[19]  Adam W Feinberg,et al.  Engineered antifouling microtopographies – correlating wettability with cell attachment , 2006, Biofouling.

[20]  S. Perni,et al.  Micropatterning with conical features can control bacterial adhesion on silicone , 2013 .

[21]  J. Y. Lim,et al.  Cell sensing and response to micro- and nanostructured surfaces produced by chemical and topographic patterning. , 2007, Tissue engineering.

[22]  John A. Jansen,et al.  Manufacturing substrate nano-grooves for studying cell alignment and adhesion , 2008 .

[23]  Adam W Feinberg,et al.  Engineered antifouling microtopographies – effect of feature size, geometry, and roughness on settlement of zoospores of the green alga Ulva , 2007, Biofouling.