DNA repair helicase: a component of BTF2 (TFIIH) basic transcription factor.

The human BTF2 basic transcription factor (also called TFIIH), which is similar to the delta factor in rat and factor b in yeast, is required for class II gene transcription. A strand displacement assay was used to show that highly purified preparation of BTF2 had an adenosine triphosphate-dependent DNA helicase activity, in addition to the previously characterized carboxyl-terminal domain kinase activity. Amino acid sequence analysis of the tryptic digest generated from the 89-kilodalton subunit of BTF2 indicated that this polypeptide corresponded to the ERCC-3 gene product, a presumed helicase implicated in the human DNA excision repair disorders xeroderma pigmentosum and Cockayne's syndrome. These findings suggest that transcription and nucleotide excision repair may share common factors and hence may be considered to be functionally related.

[1]  P. Chambon,et al.  Distinct TFIID complexes mediate the effect of different transcriptional activators. , 1993, The EMBO journal.

[2]  J. Hoeijmakers,et al.  RAD25 (SSL2), the yeast homolog of the human xeroderma pigmentosum group B DNA repair gene, is essential for viability. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[3]  F. Winston,et al.  Yeast SNF/SWI transcriptional activators and the SPT/SIN chromatin connection. , 1992, Trends in genetics : TIG.

[4]  N. Heintz,et al.  Eukaryotic replication origins as promoters of bidirectional DNA synthesis. , 1992, Trends in genetics : TIG.

[5]  P. Chambon,et al.  Cloning of the 62-kilodalton component of basic transcription factor BTF2. , 1992, Science.

[6]  M. Carlson,et al.  Yeast SNF2/SWI2, SNF5, and SNF6 proteins function coordinately with the gene-specific transcriptional activators GAL4 and Bicoid. , 1992, Genes & development.

[7]  D. Reinberg,et al.  Human general transcription factor IIH phosphorylates the C-terminal domain of RNA polymerase II , 1992, Nature.

[8]  R. Conaway,et al.  A carboxyl-terminal-domain kinase associated with RNA polymerase II transcription factor delta from rat liver. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[9]  K. D. Gulyas,et al.  SSL2, a suppressor of a stem-loop mutation in the HIS4 leader encodes the yeast homolog of human ERCC-3 , 1992, Cell.

[10]  D. Reinberg,et al.  Advances in RNA polymerase II transcription , 1992, Current Biology.

[11]  D. Reinberg,et al.  Specific interaction between the nonphosphorylated form of RNA polymerase II and the TATA-binding protein , 1992, Cell.

[12]  A. Travers.  The reprogramming of transcriptional competence , 1992, Cell.

[13]  J. Greenblatt,et al.  Recombinant TBP, transcription factor IIB, and RAP30 are sufficient for promoter recognition by mammalian RNA polymerase II. , 1992, The Journal of biological chemistry.

[14]  P. Sharp,et al.  Promoter specificity of basal transcription factors , 1992, Cell.

[15]  D. Reinberg,et al.  Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH. , 1992, The Journal of biological chemistry.

[16]  M. Horikoshi,et al.  Transcription factor TFIID induces DNA bending upon binding to the TATA element. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[17]  S. Weissman,et al.  Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF , 1992, Nature.

[18]  J. Gralla,et al.  Polymerase II promoter activation: closed complex formation and ATP-driven start site opening. , 1992, Science.

[19]  Robert Tjian,et al.  Eukaryotic coactivators associated with the TATA box binding protein , 1992 .

[20]  M. Horikoshi,et al.  Interaction of TFIID in the minor groove of the TATA element , 1991, Cell.

[21]  R. Kornberg,et al.  CTD kinase associated with yeast RNA polymerase II initiation factor b , 1991, Cell.

[22]  D. K. Hawley,et al.  TFIID binds in the minor groove of the TATA box , 1991, Cell.

[23]  D. Reinberg,et al.  The small subunit of transcription factor IIF recruits RNA polymerase II into the preinitiation complex. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[24]  P. Chambon,et al.  Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2. , 1991, The Journal of biological chemistry.

[25]  R. Roeder,et al.  Family of proteins that interact with TFIID and regulate promoter activity , 1991, Cell.

[26]  P. Chambon,et al.  Retinoic acid receptor gamma: specific immunodetection and phosphorylation , 1991, The Journal of cell biology.

[27]  R. Conaway,et al.  Initiation of eukaryotic messenger RNA synthesis. , 1991, The Journal of biological chemistry.

[28]  J. Greenblatt.  Roles of TFIID in transcriptional initiation by RNA polymerase II , 1991, Cell.

[29]  D. Reinberg,et al.  Cloning of a human gene encoding the general transcription initiation factor IIB , 1991, Nature.

[30]  S. Peterson,et al.  Promoter-dependent phosphorylation of RNA polymerase II by a template-bound kinase. Association with transcriptional initiation. , 1991, The Journal of biological chemistry.

[31]  D. Reinberg,et al.  Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex , 1990, Molecular and cellular biology.

[32]  H. Echols.  Nucleoprotein structures initiating DNA replication, transcription, and site-specific recombination. , 1990, The Journal of biological chemistry.

[33]  A. V. D. Eb,et al.  A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome , 1990, Cell.

[34]  P. Laybourn,et al.  Phosphorylation of RNA polymerase IIA occurs subsequent to interaction with the promoter and before the initiation of transcription. , 1990, The Journal of biological chemistry.

[35]  R. J. Kelleher,et al.  Resolution of factors required for the initiation of transcription by yeast RNA polymerase II. , 1990, The Journal of biological chemistry.

[36]  D. Baltimore,et al.  Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[37]  J. Hoeijmakers,et al.  Molecular cloning and biological characterization of the human excision repair gene ERCC-3 , 1990, Molecular and cellular biology.

[38]  J. T. Kadonaga.  Assembly and disassembly of the Drosophila RNA polymerase II complex during transcription. , 1990, The Journal of biological chemistry.

[39]  J. Greenblatt,et al.  Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II , 1989, Nature.

[40]  R. Conaway,et al.  An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[41]  J. Corden,et al.  Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein cdc2 , 1989, Nature.

[42]  V. Blinov,et al.  Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. , 1989, Nucleic acids research.

[43]  P. Sharp,et al.  Five intermediate complexes in transcription initiation by RNA polymerase II , 1989, Cell.

[44]  Nicolas Mermod,et al.  A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs , 1988, Nature.

[45]  T. Hodgman,et al.  A new superfamily of replicative proteins , 1988, Nature.

[46]  M. DePamphilis.  Transcriptional elements as components of eukaryotic origins of DNA replication , 1988, Cell.

[47]  R. Conaway,et al.  ATP activates transcription initiation from promoters by RNA polymerase II in a reversible step prior to RNA synthesis. , 1988, The Journal of biological chemistry.

[48]  H. Cai,et al.  Transcription initiation by RNA polymerase II in vitro. Properties of preinitiation, initiation, and elongation complexes. , 1987, The Journal of biological chemistry.

[49]  S. W. Matson,et al.  Escherichia coli Helicase I1 (uruD Gene Product) Translocates Unidirectionally in a 3’ to 5’ Direction* , 2001 .

[50]  D. K. Hawley,et al.  Separation and partial characterization of three functional steps in transcription initiation by human RNA polymerase II. , 1985, The Journal of biological chemistry.

[51]  R. Roeder,et al.  Energy requirement for specific transcription initiation by the human RNA polymerase II system. , 1984, The Journal of biological chemistry.

[52]  P. Chambon,et al.  Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences , 1983, Nature.

[53]  R. Weinmann,et al.  Mechanism of RNA polymerase II-specific initiation of transcription in vitro: ATP requirement and uncapped runoff transcripts , 1982, Cell.

[54]  K. Geider,et al.  Proteins controlling the helical structure of DNA. , 1981, Annual review of biochemistry.

[55]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.