Interferon-gamma liniment protects hairless mice against ultraviolet irradiation-induced skin damage

Ultraviolet B (UVB) irradiation has been reported as one of causes of epidermal carcinoma and also one of immunosuppressive inducers associated with an up-regulated production of T helper (Th) 2-type cytokines such as interleukin-4 (IL-4) and IL-10. We investigated using a chronically UV-irradiated mouse model in this study whether liniment of interferon-gamma (IFN-γ), one of Th1-type cytokines, would be able to protect against UV-induced skin damage or not. We report here for the first time that liniment of IFN-γ promoted desirable skin reactions such as the inhibition of epidermal cell proliferation, the increment of soluble collagen levels and the inhibition of mast cell degranulation and that the dermal reactions after IFN-γ treatment bore resemblance to the reactions induced by UVA irradiation reported to induce Th1-type immune reactions. Thus, these results suggest that liniment of IFN-γ is effective to suppress skin damages after UV irradiation through regulation of the balance of Th1-type and Th2-type immune responses.

[1]  Takeo Matsumoto,et al.  Targeted disruption of dermatopontin causes abnormal collagen fibrillogenesis. , 2002, The Journal of investigative dermatology.

[2]  C Edwards,et al.  Degenerative alterations of dermal collagen fiber bundles in photodamaged human skin and UV-irradiated hairless mouse skin: possible effect on decreasing skin mechanical properties and appearance of wrinkles. , 2001, The Journal of investigative dermatology.

[3]  D. Mitchell,et al.  Identification of a non-dividing subpopulation of mouse and human epidermal cells exhibiting high levels of persistent ultraviolet photodamage. , 2001, The Journal of investigative dermatology.

[4]  M. Norval,et al.  Differential effects of UVA1 and UVB radiation on Langerhans cell migration in mice. , 2000, Journal of photochemistry and photobiology. B, Biology.

[5]  P. Hart,et al.  Ultraviolet B-induced suppression of immune responses in interleukin-4-/- mice: relationship to dermal mast cells. , 2000, The Journal of investigative dermatology.

[6]  V. Reeve,et al.  Modulation of IL-10, IL-12, and IFN-gamma in the epidermis of hairless mice by UVA (320-400 nm) and UVB (280-320 nm) radiation. , 1999, The Journal of investigative dermatology.

[7]  K. Subbaramaiah,et al.  Regulation of Cyclooxygenase-2 by Interferon γ and Transforming Growth Factor α in Normal Human Epidermal Keratinocytes and Squamous Carcinoma Cells , 1999, The Journal of Biological Chemistry.

[8]  S. Kondo,et al.  The roles of keratinocyte-derived cytokines in the epidermis and their possible responses to UVA-irradiation. , 1999, The journal of investigative dermatology. Symposium proceedings.

[9]  N. Nishimura,et al.  Interferon-γ is Involved in Photoimmunoprotection by UVA (320–400 nm) Radiation in Mice , 1999 .

[10]  S. Ibbotson,et al.  The effects of radicals compared with UVB as initiating species for the induction of chronic cutaneous photodamage. , 1999, The Journal of investigative dermatology.

[11]  K. Jimbow,et al.  Dose‐dependent induction of IL‐12 but not IL‐10 from human keratinocytes after exposure to ultraviolet light A , 1998, Journal of cellular physiology.

[12]  Y. Takema,et al.  The Effects of UVA and UVB Irradiation on the Viscoelastic Properties of Hairless Mouse Skin in vivo , 1998, Dermatology.

[13]  S. Grether-Beck,et al.  Ultraviolet A radiation-induced expression of human genes: molecular and photobiological mechanisms. , 1997, Biological chemistry.

[14]  S. Kondo,et al.  Penetration of keratinocyte‐derived cytokines into basement membrane , 1997, Journal of cellular physiology.

[15]  K. Rajewsky,et al.  Impaired immunosuppressive response to ultraviolet radiation in interleukin-10-deficient mice. , 1996, The Journal of investigative dermatology.

[16]  I. Arany,et al.  Response of cultured cells from the epidermis and the buccal mucosa to TGF-beta 1 and comparison to interferon-gamma. , 1996, In vivo.

[17]  Y. Takema,et al.  The relationship between quantitative changes in collagen and formation of wrinkles on hairless mouse skin after chronic UV irradiation. , 1996, Journal of dermatological science.

[18]  T. Yagi,et al.  Ultraviolet-specific mutations in p53 gene in skin tumors in xeroderma pigmentosum patients. , 1993, Cancer research.

[19]  P. Zheng,et al.  UVA-induced ultrastructural changes in hairless mouse skin: a comparison to UVB-induced damage. , 1993, The Journal of investigative dermatology.

[20]  F. Urbach.  Potential health effects of climatic change: effects of increased ultraviolet radiation on man. , 1991, Environmental health perspectives.

[21]  L. Kligman,et al.  BIOCHEMICAL CHANGES IN HAIRLESS MOUSE SKIN COLLAGEN AFTER CHRONIC EXPOSURE TO ULTRAVIOLET‐A RADIATION * , 1991, Photochemistry and photobiology.

[22]  Jan C. van der Leun,et al.  Development of skin tumors in hairless mice after discontinuation of ultraviolet irradiation. , 1991 .

[23]  N. A. Kefalides,et al.  Collagen metabolism in ultraviolet irradiated hairless mouse skin and its correlation to histochemical observations. , 1989, The Journal of investigative dermatology.

[24]  F. Trautinger,et al.  UVA- and UVB-induced changes in hairless mouse skin collagen , 2004, Archives of Dermatological Research.

[25]  K. Subbaramaiah,et al.  Regulation of cyclooxygenase-2 by interferon gamma and transforming growth factor alpha in normal human epidermal keratinocytes and squamous carcinoma cells. Role of mitogen-activated protein kinases. , 1999, The Journal of biological chemistry.

[26]  N. Nishimura,et al.  Interferon-gamma is involved in photoimmunoprotection by UVA (320-400 nm) radiation in mice. , 1999, The Journal of investigative dermatology.

[27]  佐藤 まゆみ.  Ultraviolet-specific mutations in p53 gene in skin tumors in xeroderma pigmentosum patients , 1994 .